[1]洪都孜·波拉提,魏玉荣,孟肖潇,等.绵羊副结核分枝杆菌的分离与分子分型[J].中国预防兽医学报,2021,(02):134-138.[doi::10.3969/j.issn.1008-0589.202004027]
 HONGDUZI Bolati,WEI Yu-rong,MENG Xiao-xiao,et al.Isolation and molecular typing of sheep Mycobacterium aviumsubspecies paratuberculosis[J].Chinese journal of preventive veterinary medicine,2021,(02):134-138.[doi::10.3969/j.issn.1008-0589.202004027]
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绵羊副结核分枝杆菌的分离与分子分型
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2021年02
页码:
134-138
栏目:
病原生物学
出版日期:
2021-03-16

文章信息/Info

Title:
Isolation and molecular typing of sheep Mycobacterium avium
subspecies paratuberculosis
文章编号:
:1008-0589(2021)02-0134-05
作者:
 洪都孜·波拉提魏玉荣孟肖潇杨学云古努尔·吐尔逊李建军吴建勇*
 (新疆畜牧科学院兽医研究所,新疆乌鲁木齐830013)
Author(s):
 HONGDUZI Bolati WEI Yu-rong MENG Xiao-xiao YANG Xue-yun GUNUER TuerxunLI Jian-jun WU Jian-yong*
 (Institute of Veterinary Medicine, Xinjiang Academy of Animal Sciences, Urumqi 830013, China)
关键词:
:副结核分枝杆菌分离鉴定亚型鉴定分子分型绵羊
Keywords:
: Mycobacterium avium subspecies paratuberculosis isolation and identification subtype identification moleculartyping sheep
分类号:
S855.1
DOI:
:10.3969/j.issn.1008-0589.202004027
文献标志码:
A
摘要:
:为了解绵羊副结核病的病原学特征,本研究对PCR检测阳性的绵羊肠系膜淋巴结样品开展细菌分
离培养,对分离株进行形态观察,结果显示肠系膜淋巴结样品经培养后出现乳白色菌落;对分离菌株进行抗酸
染色镜检后确定为抗酸染色阳性杆菌;PCR扩增该菌IS900 基因与亚型分型片段,结果显示,IS900 基因及亚型分
型检测为Ⅱ型(牛型)副结核分枝杆菌(MAP);多位点短重复序列(MLSSR)和分枝杆菌散在分布重复单位-可变数
目串联重复序列(MIRU-VNTR)分子分型结果显示,MLSSR和MIRU-VNTR分子分型数字代码分别为7+555555555
和32632129。以上结果表明本研究分离的MAP 是一种未曾报道的新基因型菌株,为首次在我国分离的绵羊源
II型MAP。本研究为进一步开展绵羊MAP病原学和流行病学调查提供了参考资料。
Abstract:
To understand the etiological characteristics of sheep Mycobacterium avium subspecies paratuberculosis (MAP), the
sheep mesenteric lymph node sample positive for PCR detection was carried out bacteria isolation, the isolate was detected by the
colony morphology observation, and the results showed that milky white colonies appeared after the mesenteric lymph node sample
was cultured. The acid-fast staining and microscopic examination showed that the isolate was a positive bacillus, PCR amplification
of the IS900 gene and subtype fragment showed that the IS900 and subtype genes were detected as type Ⅱ (cattle type) MAP. Multilocus
short sequence repeat (MLSSR) and mycobacterial interspersed repetitive unit- variable number of tandem repeat (MIRUVNTR)
molecular typing showed that the numerical codes for MLSSR and MIRU-VNTR molecular typing were 7+555555555 and
32632129, respectively. The above results showed that the isolate was a novel genotype that had never been reported, indicating
isolation of a type II (cattle type) MAP from sheep for the first time in China. The study provides reference for further etiological
and epidemiological investigation of sheep MAP.

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更新日期/Last Update: 2021-03-17