[1]陈静?,王军?,岳筠,等.绵羊肺炎支原体GZ-WN 株感染不同品种羊的组织病变与病原核酸载量差异的研究[J].中国预防兽医学报,2021,(02):128-133.[doi::10.3969/j.issn.1008-0589.202005004]
 CHEN Jing,WANG Jun,YUE Jun,et al.Histopathological changes and pathogen nucleic acid load indifferent breeds of sheep infected with Mycoplasma pneumoniaeGZ-WN strain[J].Chinese journal of preventive veterinary medicine,2021,(02):128-133.[doi::10.3969/j.issn.1008-0589.202005004]
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绵羊肺炎支原体GZ-WN 株感染不同品种羊的组织病变
与病原核酸载量差异的研究
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2021年02
页码:
128-133
栏目:
病原生物学
出版日期:
2021-03-16

文章信息/Info

Title:
Histopathological changes and pathogen nucleic acid load in
different breeds of sheep infected with Mycoplasma pneumoniae
GZ-WN strain
文章编号:
:1008-0589(2021)02-0128-06
作者:
 陈静1?王军1?岳筠3张双翔3谢晓东1李涛3文明12程振涛12*
 (1. 贵州大学动物科学学院,贵州贵阳550025;2. 贵州省动物疫病与兽医公共卫生重点实验室,贵州贵阳550025;
3. 贵州省动物疫病预防控制中心,贵州贵阳550008)
Author(s):
CHEN Jing1?WANG Jun1? YUE Jun3 ZHANG Shuang-xiang3 XIE Xiao-dong1 LI Tao3WEN Ming12 CHENG Zhen-tao12*
 (1. College of Animal Science, Guizhou University, Guiyang 550025, China; 2. Key Laboratory of Animal Diseases and Veterinary Public Health in
Guizhou Province, Guiyang 550025, China; 3. Animal Disease Prevention and Control Center in Guizhou Province, Guiyang 550008, China)
关键词:
:绵羊肺炎支原体不同品种核酸载量差异性分析
Keywords:
: Mycoplasma pneumoniae different breeds sheep nucleic acid load difference analysis
分类号:
S852.62
DOI:
:10.3969/j.issn.1008-0589.202005004
文献标志码:
A
摘要:
为探究绵羊肺炎支原体(Mo)致不同品种羊感染及病原核酸载量的差异,本研究利用Mo贵州威宁
(GZ-WN)株分别感染5个品种羊,感染后死亡或迫杀后观察各组织的剖检病变,经荧光定量PCR检测各组织Mo
的核酸载量,统计分析相同品种羊不同组织中Mo的核酸载量及不同品种羊相同组织中的核酸载量。结果显示,
Mo可引起5个品种羊感染,死亡率呈现白山羊、黑山羊>麻羊>波尔山羊>湖羊;Mo感染可引起5个品种羊的肺
脏、气管和胸腔出现不同程度的剖检病变,黑山羊肺脏粘连与白山羊气管黏液病变较其他品种羊严重;白山羊
胸腔积液较其它品种羊严重,黑山羊次之;5个品种羊的心脏、肝脏、脾脏及肾脏均无肉眼可见病变。相同品种
羊不同组织中Mo核酸载量的统计分析结果显示,相同品种羊的不同组织Mo核酸载量不同,各品种羊气管中的
Mo核酸载量相对最高,极显著高于其他组织的核酸载量(p<0.01);其次是肺脏,而脾脏和肾脏中的Mo载量较
低。不同品种羊相同组织中的Mo核酸载量统计结果显示,5个品种羊气管中Mo核酸载量最多的是波尔山羊,最
少的是白山羊,且极显著低于其他品种羊(p<0.01);黑山羊肺脏中Mo 核酸载量显著高于其他品种羊(p<0.05),
湖羊Mo载量最少;心脏组织中,黑山羊Mo的核酸载量显著高于除麻羊以外的其他品种羊(p<0.05),载量最少的
是白山羊;肝脏组织中,Mo核酸载量最多的是麻羊,显著高于除湖羊以外的其他品种羊(p<0.05),而白山羊最
少;脾脏组织中, Mo核酸载量最多的是湖羊,显著高于其他品种羊(p<0.05),最少的是麻羊。肾脏组织中,Mo
核酸载量最多的是湖羊,显著高于除波尔山羊外的其他品种羊(p<0.05),最少的是麻羊。上述结果表明,Mo感
染相同品种羊存在组织病变和组织核酸载量的差异,且感染羊脏器组织Mo的核酸载量与组织病变程度和临床表
现呈正相关;不同品种羊感染Mo后相同组织的Mo核酸载量也不同。Mo人工感染不同品种羊组织病变与核酸载
量的差异研究国内外至今无相关报道,本研究为探究Mo对不同品种羊感染差异性机制提供参考依据。
Abstract:
In order to e explore the infection outcomes and pathogen nucleic acid loads in different breeds of sheep infected
with Mycoplasma pneumoniae (Mo), 5 sheep breeds were infected with Mo Guizhou Weining (GZ-WN) strain. The pathological
changes of each tissue were observed after infection or sacrificing, and the nucleic acid load of Mo in each tissue was detected by
fluorescence quantitative PCR. The nucleic acid load of Mo in different tissues of the same breed of sheep and the nucleic acid load
of the same tissue of different breeds of sheep were statistically analyzed. The results showed that Mo could cause infection in five
breeds of sheep, and the mortality rate ranks as follows: white goat≈black goat>Ma sheep>Boer goat>Hu sheep. Mo infection
could cause pathological changes of different degrees in lung, trachea and thorax of five breeds of sheep; lung adhesion and
tracheal mucus lesion of black goat was more serious than that of other breeds; pleural effusion of white goat was more serious
than that of other breeds, followed by black sheep. The heart, liver, spleen and kidney of the five breeds of sheep were not visible
to the naked eye. The results of statistical analysis of Mo nucleic acid load in different tissues of the same sheep breed showed that
the Mo nucleic acid load was different in different tissues of the same breed, and the Mo nucleic acid load in the trachea of each
breed was the highest, which was significantly higher than that in other tissues (p<0.01), followed by lung, while Mo load in spleen
and kidney was lower. The statistical results of Mo nucleic acid load in the same tissue of different breeds of sheep showed that the
Mo nucleic acid load in the trachea of the five breeds was the highest in Boer goat and the least in white goat, which was
significantly lower than that in other breeds (p<0.01); the Mo nucleic acid load in lung of black goat was significantly higher than
that of other breeds (p<0.05); and the Mo load of Hu sheep was the lowest. In heart tissue, the nucleic acid load of Mo in black
goat was significantly higher than that in other breeds of sheep except Ma sheep (p<0.05), and the lowest was white goat. In liver
tissue, the nucleic acid load of Mo in Ma sheep was the highest, which was significantly higher than that in other breeds except Hu
sheep (p<0.05), while that in white goat was the least. In spleen, the nucleic acid load of Mo was the highest in Hu sheep,
significantly higher than that in other breeds of sheep (p<0.05), and the least in Ma sheep. In kidney tissue, the Mo nucleic acid
load of Hu sheep was the highest, which was significantly higher than that of other breeds of sheep except Boer goat (p<0.05), and
the least was in Ma sheep. The above results showed that there were differences in tissue pathological changes and tissue nucleic
acid load of Mo infected sheep of the same breed, and the nucleic acid load of Mo in infected sheep organs was positively
correlated with the degree of tissue pathological changes and clinical manifestations. The Mo nucleic acid load of the same tissue
was different in different breeds of sheep infected with Mo. To date, there has been no domestic or foreign studies on the difference
of histopathology and nucleic acid load of artificial infection with Mo in different breeds of sheep. The results of this study provide
a reference for exploring the mechanism of different infection outcomes by Mo in different breeds of sheep.

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更新日期/Last Update: 2021-03-17