[1]李 玉,穷 达,张 敏,等.猪A群轮状病毒RVA/Pig-tc/CHN/SWU-1C/2018/G9P[13]株的分离与鉴定[J].中国预防兽医学报,2019,(11):1170-1173.[doi:0.3969/j.issn.1008-0589.201903008]
 LI Yu,QIONG Da,ZHANG Min,et al.Isolation and identification of the porcine group A rotavirus strain of RVA/Pig-tc/CHN/SWU-1C/2018/G9P[13][J].Chinese journal of preventive veterinary medicine,2019,(11):1170-1173.[doi:0.3969/j.issn.1008-0589.201903008]
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猪A群轮状病毒RVA/Pig-tc/CHN/SWU-1C/2018/G9P[13]株的分离与鉴定()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2019年11
页码:
1170-1173
栏目:
研究简报
出版日期:
2019-12-25

文章信息/Info

Title:
Isolation and identification of the porcine group A rotavirus strain of RVA/Pig-tc/CHN/SWU-1C/2018/G9P[13]
文章编号:
1008-0589(2019)11-1170-04
作者:
 

李 玉1穷 达13张 敏1阚蕊慈1汤 承12岳 华12张 斌12*

 (1. 西南民族大学 生命科学与技术学院,四川 成都 610041;2. 国家民族事务委员会青藏高原动物疫病防控创新团队,
四川 成都 610041;3. 西藏自治区生物药品制造厂,西藏 拉萨 800003)
Author(s):
 

LI Yu1 QIONG Da13 ZHANG Min1 KAN Rui-ci1 TANG Cheng12 YUE Hua12 ZHANG Bin12*

 (1. College of Life Science and Technology,SouthwestMinzu University, Chengdu 610041, China; 2. Animal Disease Prevention and Control Innovation Team in the Qinghai-Tibetan Plateau of State Ethic Affairs Commission, Chengdu 610041, China;
3. Biological drug manufacturing factory of Tibet autonomous region, Lhasa 800003, China)
关键词:
猪轮状病毒分离鉴定基因组分析重组
Keywords:
 Porcine rotavirus  isolation  identification  genome analysis  reassortment
分类号:
S852.65
DOI:
0.3969/j.issn.1008-0589.201903008
文献标志码:
A
摘要:
为分离并鉴定A群猪轮状病毒(PoRV),本研究通过MA-104细胞分离培养、间接免疫荧光鉴定、RT-PCR鉴定以及序列分析对一份疑似PoRV感染的小肠样本进行了病毒的分离与鉴定。结果显示,分离到一株PoRV,其基因型为G9-P[13]-I5-R1-C1-M1-A8-NI-T7-E1-H1,命名为RVA/Pig-tc/CHN/SWU-1C/2018/G9P[13]。经序列分析显示,该病毒的VP7、VP4、VP6、NSP1、NSP2、NSP4和NSP5基因与PoRV相似性最高,达92.9 %~98.3 %,而VP1~VP3、NSP3基因与人源A群轮状病毒(GAR)相似性最高,达94.1 %~97.3 %,表明该病毒为一株人猪重配的PoRV。经软件重组分析显示,该病毒的VP2基因在626 bp处与人源GAR R1207株发生重组,进一步证实该病毒为人猪重组病毒株。本研究首次在中国大陆报道分离鉴定到一株猪源人猪重配的G9P[13]型PoRV,为后续对其致病性研究奠定了基础。
Abstract:
To isolate and identify the porcine group A rotavirus (PoRV), a small intestine sample suspected of PoRV infection was isolated using MA-104 cells and was further identified by indirect immunofluorescence assay, RT-PCR and sequence analysis. The results showed that the virus isolate was PoRV, which genome constellation was G9-P[13]-I5-R1-C1-M1-A8-NI-T7-E1-H1, and named as RVA/Pig-tc/CHN/SWU-1C/2018/G9P[13]. Sequence analysis showed that the homology of VP7, VP4, VP6, NSP1, NSP2, NSP4 and NSP5 genes of the isolate had the highest identities with PoRV, with 92.9%-98.3% similarity, while VP1-VP3 and NSP3 genes had the highest homologies with human group A rotavirus (GAR), with 94.1%-97.3% similarity. The above results indicated that this rotavirus strain was a reassortant between human and porcine rotavirus. Recombination analysis showed that the VP2 gene of the virus recombined with human GAR R1207 strain at 626bp, which further confirmed that the virus was a human and porcine reassortant strain. In addition, this is the first time isolated and identified a reassortant G9P[13] PoRV between human and porcine strains in mainland China, which lays a foundation for further pathogenicity research.

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(本文编辑:彭永刚;英文编辑:孟凡丹)

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更新日期/Last Update: 2019-12-10