[1]范林进,王雨龙,吴甜甜,等.我国传染性法氏囊病病毒新型变异株分析研究[J].中国预防兽医学报,2019,(11):1164-1168.[doi:0.3969/j.issn.1008-0589.201907028]
 FAN Lin-jin,WANG Yu-long,WU Tian-tian,et al.The prevalence of novel variant strains of infectious bursal disease virus in China[J].Chinese journal of preventive veterinary medicine,2019,(11):1164-1168.[doi:0.3969/j.issn.1008-0589.201907028]
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我国传染性法氏囊病病毒新型变异株分析研究()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2019年11
页码:
1164-1168
栏目:
研究简报
出版日期:
2019-12-25

文章信息/Info

Title:
The prevalence of novel variant strains of infectious bursal disease virus in China
文章编号:
1008-0589(2019)11-1164-06
作者:
 

范林进12王雨龙12吴甜甜12李 凯12姜 楠12高玉龙12高 立12刘长军1崔红玉12潘 青1张艳萍1刘玉凤3孙先本3刘俊启3王笑梅124*祁小乐12*

 (1. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/禽传染病研究室,黑龙江 哈尔滨 150069;
2. 世界动物卫生组织传染性法氏囊病参考实验室,黑龙江 哈尔滨 150069;3. 法国诗华动物保健公司,北京 100102;
4. 江苏高校动物重要疫病与人畜共患病协同创新中心,江苏 扬州 225009)
Author(s):
 

FAN Lin-jin12 WANG Yu-long12 WU Tian-tian12 LI Kai12 JIANG Nan12 GAO Yu-long12 GAO Li12LIU Chang-jun1 CUI Hong-yu12 PAN Qing1 ZHANG Yan-ping1 LIU Yu-feng3 SUN Xian-ben3LIU Jun-qi3 WANG Xiao-mei124* QI Xiao-le12*

 (1. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Harbin 150069, China; 2. International Epizootic Office (OIE) Reference Laboratory for Infectious Bursal Disease, Harbin 150069, China; 3. Ceva Animal Health Service Company, Beijing 100102, China; 4. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China)
关键词:
非典型传染性法氏囊病传染性法氏囊病病毒新型变异株法氏囊半数萎缩量
Keywords:
 atypical infectious bursal disease  infectious bursal disease virus  the novel variant strain  50% bursae atrophy dose
分类号:
S852.65
DOI:
0.3969/j.issn.1008-0589.201907028
文献标志码:
A
摘要:
近期非典型传染性法氏囊病(IBD)给养禽业带来了新的威胁,本研究团队率先鉴定发现其病原为IBDV新型变异株。为了解该IBDV新型变异株流行规律和致病特点,本研究对2018年9月~2019年6月分离自黑龙江、辽宁、河北、山东、江苏、浙江、云南7个省的32个IBDV代表株的VP2基因高变区进行了遗传演化分析,结果显示,IBDV新型变异株虽然属于变异株分支,但却不同于美国早期变异株,其形成了一个独立的亚群。动物回归试验结果显示,IBDV新型变异株不致死鸡,但造成其中枢免疫器官法氏囊严重萎缩,B淋巴细胞崩解坏死,复制出了非典型IBD的症状。本研究测定了IBDV新型变异株的最低致病剂量,并依据病毒自身特殊性状,首次提出用法氏囊半数萎缩量(BAD50)作为IBDV新型变异株的病毒效价单位。本研究为IBDV新型变异株的流行规律监测和综合防控提供了数据支撑。
Abstract:
Atypical infectious bursal disease (IBD) had brought new threats to poultry industry in recent years, and its pathogen as a novel variant IBDV had been identified for the first time by our laboratory. To investigate the epidemic and pathogenic features, the epidemiological investigation was performed from September 2018 to June 2019, and the prevalence of the novel variant IBDVswere detectedin 7 provinces including Heilongjiang, Liaoning, Hebei, Shandong, Jiangsu, Zhejiang and Yunnan, So far, the novel variant IBDVs has been detectablein at least 13 provinces and cities in China. The genetic evolution analysis of the VP2 gene hypervariable regionwas carried out for the obtained 32 representative strains. The results indicated that although the novel variant IBDVs belonged to the branch of variant IBDV, they were different from the early American variant IBDV but formed an independent subgroup. The animal regression test indicated that although no obvious clinical appearance, the novel variant IBDV caused severe atrophy of the bursa in the central immune organ,collapse and necrosis of B lymphocytes, which confirmed the symptoms of atypical IBD. In this study, the minimum pathogenic dose of the novel variant IBDV was also evaluated, and according to the special characteristics of the virus infection, the usage of 50% bursae atrophy dose (BAD50) was proposed for the first time as the viral titer unit of the novel variant IBDV. This study provided a basis for the epidemic monitoring, comprehensive prevention and control of novel variant IBDVs.

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(本文编辑:彭永刚;英文编辑:胡   哲)

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更新日期/Last Update: 2019-12-10