[1]臧鑫鑫?,党光辉?,王忠星,等.耻垢分枝杆菌诱导中性粒细胞胞外诱捕网形成的研究[J].中国预防兽医学报,2019,(11):1154-1158.[doi:0.3969/j.issn.1008-0589.201905007]
 ZANG Xin-xin?,DANG Guang-hui?,WANG Zhong-xing,et al.Formation of neutrophil extracellular traps induced by Mycobacterium smegmatis[J].Chinese journal of preventive veterinary medicine,2019,(11):1154-1158.[doi:0.3969/j.issn.1008-0589.201905007]
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耻垢分枝杆菌诱导中性粒细胞胞外诱捕网形成的研究()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2019年11
页码:
1154-1158
栏目:
免疫学
出版日期:
2019-12-25

文章信息/Info

Title:
Formation of neutrophil extracellular traps induced by Mycobacterium smegmatis
文章编号:
1008-0589(2019)11-1154-05
作者:
 

臧鑫鑫?党光辉?王忠星刘虹秀崔子寅崔莹莹宋宁宁刘思国*

 (中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/动物细菌病创新团队,黑龙江 哈尔滨 150069)
Author(s):
 ZANG Xin-xin?覮 DANG Guang-hui?覮 WANG Zhong-xing LIU Hong-xiu CUI Zi-yin CUI Ying-ying SONG Ning-ning LIU Si-guo*
 

(1. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,ZANG Xin-(1. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,
Chinese Academy of Agricultural Sciences, Harbin 150069, China)

关键词:
耻垢分枝杆菌中性粒细胞中性粒细胞胞外诱捕网
Keywords:
Mycobacteriu smegmatis  neutrophil  neutrophils extracellular traps
分类号:
S852.61
DOI:
0.3969/j.issn.1008-0589.201905007
文献标志码:
A
摘要:
中性粒细胞胞外诱捕网(NETs)是中性粒细胞抵御病原微生物重要的防御机制之一,NETs能够在体内外条件下捕获并杀灭病原微生物。为研究模式菌株-耻垢分枝杆菌(MS)与中性粒细胞的相互作用,本研究通过分离牛外周血中性粒细胞,将标记红色荧光的MS与中性粒细胞共孵育,通过激光共聚焦观察NETs的形成;采用Pi-coGreenR dsDNA试剂盒定量分析胞外dsDNA的释放量;并且通过菌落计数统计MS的存活情况。结果显示,MS能够诱导中性粒细胞形成NETs,胞外游离DNA的释放量随着时间的延长而逐渐增多,与对照组相比差异极显著(p<0.01或p<0.001),且NETs能够杀灭MS。本研究为后续利用MS作为模式菌研究结核分枝杆菌重要蛋白功能的研究提供参考依据。
Abstract:
Neutrophil Extracellular Traps (NETs) is one of the important immune defence mechanisms for neutrophils to resist pathogenic microbes. Pathogens are entraped and killed by NETs in vitro and vivo. To study the interaction between the model strain-Mycobacterium smegmatis (MS) and neutrophils, MS marked by red fluorescence was incubated with neutrophils to observe the formation of NETs by confocal microscopy and quantitive analysis of the releasing extracellular free DNA by Pi-coGreenR dsDNA kit. Further, MS survival was measured by counting the colony formation units. The results showed that MS could induce the formation of NETs, the release of extracellular DNA gradually increased with the time showed extremely significant difference compared to the control group (p<0.01 or p<0.001), and MS was killed by NETs. This work provides an experimental reference for future research of the important protein function of Mycobacterium tuberculosis (MTB) by MS model.

参考文献/References:

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  (本文编辑:李 娜;英文编辑:于申业)

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更新日期/Last Update: 2019-12-10