[1]肖 芳,嵇辛勤*,李基棕*,等.山羊副流感病毒3型感染MDBK细胞和BECs 后7种ISGs mRNA变化分析[J].中国预防兽医学报,2019,(11):1147-1153.[doi:0.3969/j.issn.1008-0589.201904014]
 XIAO Fang,JI Xin-qin*,LI Ji-zong*,et al.Analysis on the change of seven ISGs transcription in MDBK cells and BECs infected with Caprine parainfluenza virus type 3[J].Chinese journal of preventive veterinary medicine,2019,(11):1147-1153.[doi:0.3969/j.issn.1008-0589.201904014]
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山羊副流感病毒3型感染MDBK细胞和BECs 后7种ISGs mRNA变化分析()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2019年11
页码:
1147-1153
栏目:
免疫学
出版日期:
2019-12-25

文章信息/Info

Title:
Analysis on the change of seven ISGs transcription in MDBK cells and BECs infected with Caprine parainfluenza virus type 3
文章编号:
1008-0589(2019)11-1147-07
作者:
 

肖 芳12嵇辛勤1*李基棕2*廖 政12毛 立2李文良2孙 敏2刘茂军2

 (1. 贵州大学 动物科学学院,贵州 贵阳 550025;
2. 江苏省农业科学院兽医研究所 农业部兽用生物制品工程技术重点实验室,江苏 南京 210014)
Author(s):
 

XIAO Fang12 JI Xin-qin1* LI Ji-zong2* LIAO Zheng12 MAO Li2 LI Wen-liang2 SUN Min2 LIU Mao-jun2

 (1. College of Animal Science of Guizhou University, Guizhou Province, Guiyang 550025, China; 2. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Province, Nanjing 210014, China)
关键词:
 干扰素刺激基因SYBR GreenⅠ荧光定量PCR山羊副流感病毒3型建立及应用
Keywords:
 interferon stimulated genes  SYBR GreenⅠ real-time PCR  caprine parainfluenza virus type 3  establishment and application
分类号:
S852.61
DOI:
0.3969/j.issn.1008-0589.201904014
文献标志码:
A
摘要:
为探究山羊副流感病毒3型(CPIV3)感染MDBK细胞和气管上皮细胞(BECs)后的抗病毒免疫应答,本研究针对7种干扰素刺激基因(ISGs):IFI6、ISG15、OAS1Y、OAS1Z、Mx1、Mx2及RSAD2的基因序列设计特异性引物,经优化建立了检测7种ISGs的SYBR Green I 荧光定量PCR方法。利用所建立的方法检测CPIV3 JS2013株感染MDBK细胞和BECs 24 h、48 h和72 h后7种ISGs的mRNA 转录水平。结果显示,在MDBK细胞中,CPIV3感染时间越长,7种ISGs的转录水平上调倍数越高,其中ISG15、Mx1、Mx2和RSAD2的mRNA转录水平上调量持续高于IFI6、OAS1Y和OAS1Z;而在BECs中,CPIV3感染后虽然IFI6、ISG15、OAS1Y、OAS1Z、Mx1、Mx2和RSAD2 mRNA转录水平均出现上调,但ISG15、Mx2和RSAD2的mRNA转录水平上调量高于IFI6、OAS1Y、OAS1Z和Mx1。结果表明,CPIV3 JS2013株可以刺激MDBK细胞和BECs中这7种ISGs的大量转录,本研究为进一步探究其抗病毒功能提供了实验依据。
Abstract:
To investigate the antiviral immune response of caprine parainfluenza virus type 3 (CPIV3) infected with  MDBK cells and bronchial epithelial cells (BECs) , we designed the primers and developed the real-time PCR assays based on SYBR Green Ⅰ for 7 interferon-stimulated genes (ISGs): IFI6, ISG15, OAS1Y, OAS1Z, Mx1, Mx2 and RSAD2 genes. The mRNA transcript levels of seven ISGs of MDBK cells and BECs infected with CPIV3 JS2013 strain at 24 h, 48 h and 72 h were detected by the real-time PCR assays. The results showed that the longer the infection time in MDBK cells, the higher the transcriptional up-regulation of the seven ISGs, and the up-regulation of ISG15, Mx1, Mx2 and RSAD2 was higher than that of IFI6, OAS1Y and OAS1Z. But in BECs, although the transcriptional levels of IFI6, ISG15, OAS1Y, OAS1Z, Mx1, Mx2 and RSAD2 mRNA were up-regulated, the transcriptional levels of ISG15, Mx2 and RSAD2 were higher than those of IFI6, OAS1Y, OAS1Z and Mx1. The results showed that the CPIV3 JS2013 strain can stimulate the large-scale transcription of  these seven ISGs in MDBK cells and BECs, which provids experimental basis for further study of its antiviral function.

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                                 (本文编辑:李 娜;英文编辑:高玉龙)

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更新日期/Last Update: 2019-12-10