[1]魏春华,夏　伟,马　英,等.福建省14株PRRSV-2全基因组特性分析[J].中国预防兽医学报,2019,(09):964-968.[doi:0.3969/j.issn.1008-0589.201812010]
　WEI Chun-hua,XIA Wei,MA Ying,et al.Genetic characterization of 14 porcine reproductive and respiratory syndrome virus-2 isolates from Fujian province[J].Chinese journal of preventive veterinary medicine,2019,(09):964-968.[doi:0.3969/j.issn.1008-0589.201812010]
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福建省14株PRRSV-2全基因组特性分析()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2019年09
页码:
964-968
栏目:
研究简报
出版日期:
2019-10-25

文章信息/Info

Title:
Genetic characterization of 14 porcine reproductive and respiratory syndrome virus-2 isolates from Fujian province
文章编号:
1008-0589(2019)09-0964-05
作者:
 

魏春华; 夏　伟; 马　英; 李　娜; 吴熠丹; 勾明郗; 林志峰; 戴爱玲; 杨小燕*; 刘建奎*

 (龙岩学院生命科学学院 福建省家畜传染病防治与生物技术重点实验室/福建省生猪疫病防控工程技术研究中心，
福建 龙岩 364000)
Author(s):
 

WEI Chun-hua;  XIA Wei;  MA Ying;  LI Na;  WU Yi-dan;  GOU Ming-xi;  LIN Zhi-feng;  DAI Ai-ling; YANG Xiao-yan*;  LIU Jian-kui*

 (College of Life Sciences of Longyan University, Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Fujian Engineering Research Center for Swine Disease Control and Prevention, Longyan 364000, China)
关键词:
猪繁殖与呼吸综合征病毒; 全基因组; 遗传变异; 基因重组
分类号:
S852.65
DOI:
0.3969/j.issn.1008-0589.201812010
文献标志码:
A
摘要:
为了解福建省PRRSV的流行情况及分子特征，本研究选取14株不同年份不同谱系的PRRSV-2 (lineage 3、lineage 8.7、lineage 5.1和lineage 1)进行全基因组扩增并测序分析。结果显示，福建省14株PRRSV-2基因组全长为15 016 bp～15 407 bp，与VR-2332、CH-1a、JXA1和NADC30的核苷酸序列同源性为82.9 %～99.4 %，14株PRRSV-2之间核苷酸序列同源性为82.1 %～99.3 %，差异较大。分离株FJCH、FJZH与JXA1-R疫苗株高度同源(99.5 %～99.6 %)，且处于同一进化分支，推测其可能来源于疫苗株。重组分析显示14株PRRSV-2存在较高的重组概率(3/14)，均发生在lineage 8.7和lineage 1之间。本研究为科学防控福建地区PRRS提供了参考数据。
　　
Abstract:
In order to investigate the prevalence and genetic characterization of PRRSV in Fujian province, 14 isolates of different lineage of PRRSV-2 (lineage 3, lineage 8.7, lineage 5.1 and lineage 1) were selected for complete viral genome analysis. The results showed that the genome lengths of 14 PRRSV-2 isolates were ranged from 15,016-15,407 bp, and shared 82.9%-99.4% nucleotide identity with VR-2332, CH-1a, JXA1 and NADC30, and 82.1%-99.3% among themselves. Genomic sequence analysis also showed that FJCH and FJZH isolates displayhigh homology (99.5%-99.6%) with JXA1-R, which meand the two isolates probably originated from JXA1-R MLV strain. Genome recombination analysis showed that the 14 PRRSV-2 isolates had the high recombination rate (3/14) between lineage 8.7 and lineage 1 virus. This study provides atheoretical basis for PRRS prevention and control.
Key words:  porcine reproductive and respiratory syndrome virus;  complete viral genome;  genetic variation;  recombination

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                  (本文编辑：彭永刚；英文编辑：王　刚)

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更新日期/Last Update: 2019-10-29