[1]关立增,王 亨,刘俊俊,等.硒对金黄色葡萄球菌感染的奶牛乳腺上皮细胞Nod2/MAPK/mTORs信号通路中关键因子mRNA转录水平的影响[J].中国预防兽医学报,2019,(09):879-884.[doi:0.3969/j.issn.1008-0589.201811036]
 GUAN Li-zeng,WANG Heng,LIU Jun-jun,et al.The effect of Selenium on the transcriptional level of key factors in Nod2/MAPK/mTORs signaling pathways in S.aureus infected bovine mammary epithelial cells[J].Chinese journal of preventive veterinary medicine,2019,(09):879-884.[doi:0.3969/j.issn.1008-0589.201811036]
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硒对金黄色葡萄球菌感染的奶牛乳腺上皮细胞Nod2/MAPK/mTORs信号通路中关键因子
mRNA转录水平的影响
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2019年09
页码:
879-884
栏目:
病原生物学
出版日期:
2019-10-25

文章信息/Info

Title:
The effect of Selenium on the transcriptional level of key factors in Nod2/MAPK/mTORs signaling pathways in S.aureus infected bovine mammary epithelial cells
文章编号:
1008-0589(2019)09-0879-06
作者:
 

关立增1王 亨23刘俊俊23王 娟1韩照清1毕崇亮1*

 (1. 临沂大学 农林科学学院,山东 临沂 276005;2. 扬州大学 兽医学院,江苏 扬州 225009;
3. 江苏省动物重要疫病与人兽共患病防控协调创新中心,江苏 扬州 225009)
Author(s):
 

GUAN Li-zeng1 WANG Heng23 LIU Jun-jun23 WANG Juan1 HAN Zhao-qing1 BI Chong-liang1*

 (1. College of Agriculture and Forestry Science, Linyi University, Linyi 276005, China; 2. College of Veterinary Medicine,
Yangzhou University, Yangzhou 225009, China; 3. Jiangsu Co-innovation Center for  Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China)
关键词:
 金黄色葡萄球菌Nod2/MAPK/mTORs奶牛乳腺上皮细胞mRNA
Keywords:
  Selenium  S.aureus  Nod2/MAPK/mTORs  bMECs  mRNA
分类号:
S852.61
DOI:
0.3969/j.issn.1008-0589.201811036
文献标志码:
A
摘要:
为探究硒(Se)对金黄色葡萄球菌(S.aureus)感染的奶牛乳腺上皮细胞(bMECs) Nod2/MAPK/mTORs信号通路的调控机制,本研究首先用不同浓度硒(2 μmol/L、4 μmol/L和8 μmol/L)对bMECs进行预孵育,12 h后再经S.aureus感染处理。分别于感染后6 h、8 h和10 h收集bMECs提取其RNA,应用qPCR方法检测bMECs中Nod2、RIP2、JNK、AKT、mTOR、IL-8和IL-10 mRNA的转录水平。结果显示,S.aureus能显著提高bMECs中Nod2、RIP2、JNK、AKT和mTOR mRNA的转录水平(p<0.01),而硒能不同程度的抑制这些因子mRNA的转录水平(p<0.05或p<0.01)。此外,S.aureus能显著或极显著提高bMECs中IL-8和 IL-10 mRNA的转录水平(p<0.05或p<0.01),而硒对S.aureus感染的bMECs中IL-8和IL-10 mRNA的转录水平有明显抑制作用(p<0.05或p<0.01)。上述结果表明,硒可通过抑制Nod2/MAPK/mTORs信号通路的转导而减轻S.aureus诱导的bMECs的炎症反应。本研究为阐明硒能减轻S.aureus诱导的bMECs炎症反应的机制提供试验依据。
  
Abstract:
To study the regulation mechanism of Selenium (Se) on Nod2/MAPK/mTORs signaling pathway in the bovine mammary epithelial cells (bMECs) infected by S.aureus, bMECs were pre-incubated with different concentrations of Se (2 μmol/L, 4 μmol/L and 8 μmol/L) for 12h and then infected with S.aureus. bMECs were collected 6h, 8h and 10h post S.aureus infection to extract total RNA. The transcriptional level of Nod2, RIP2, JNK, AKT, mTOR, IL-8 and IL-10 mRNA in bMECs were detected by qPCR. Results showed that S.aureus infection could significantly increase the mRNA transcriptional level of Nod2, RIP2, JNK,AKT and mTOR in bMECs (p<0.01). But Se could inhibit the mRNA transcriptional level of the above factors in different degrees (p<0.05 or p<0.01). In addition, S.aureus infection could also significantly increase the mRNA transcriptional level of IL-8 and IL-10 in bMECs (p<0.05 or p<0.01), but Se could significantly inhibit the mRNA transcriptional level of inflammatory cytokine IL-8 and IL-10 in S.aureus infected bMECs (p<0.05 or p<0.01). The above results suggested that Se could alleviate inflammatory reaction of bMECs induced by S.aureus by inhibiting the transduction of Nod2/MAPK/mTORs signaling pathway. This study will provide a theoretical basis for elucidating the specific mechanism of Se alleviating inflammation of bMECs induced by S.aureus.

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(本文编辑:彭永刚;英文编辑:张振宇)

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更新日期/Last Update: 2019-10-25