[1]严 楠,冯 帆,李明翔,等.四川部分地区犬圆环病毒的病原检测及基因组特征[J].中国预防兽医学报,2019,(03):313-316.[doi:0.3969/j.issn.1008-0589.201809010]
 YAN Nan,FENG Fan,LI Ming-xiang,et al.Detection and genome characteristics of canine circovirus in part areas of Sichuan[J].Chinese journal of preventive veterinary medicine,2019,(03):313-316.[doi:0.3969/j.issn.1008-0589.201809010]
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四川部分地区犬圆环病毒的病原检测及基因组特征()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2019年03
页码:
313-316
栏目:
研究简报
出版日期:
2019-04-25

文章信息/Info

Title:
Detection and genome characteristics of canine circovirus in part areas of Sichuan
文章编号:
1008-0589(2019)03-0313-04
作者:
 

严 楠冯 帆李明翔汤 承岳 华*

 (西南民族大学 生命科学与技术学院,四川 成都 610041)
Author(s):
 

YAN Nan FENG Fan LI Ming-xiang TANG Cheng YUE Hua*

 (College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China)
关键词:
犬圆环病毒宠物犬流行基因组
Keywords:
  canine circovirus  pet dog  prevalence  genome
分类号:
S852.65
DOI:
0.3969/j.issn.1008-0589.201809010
文献标志码:
A
摘要:
为调查犬圆环病毒(CanineCV)在四川部分地区的流行情况及基因组特征,本研究采用荧光定量PCR方法检测了2015年~2017年采集自四川6家宠物医院的208份腹泻犬粪便样品。结果显示CanineCV的平均阳性率为15.4 % (32/208),3年间的检出率无明显变化;并且阳性样品均与一种或多种病原共感染。获得了6条完整的CanineCV Rep基因序列及1株CanineCV (CD17/2016)全基因组序列,进化分析显示CanineCV四川株(CD17/2016)与GenBank中的国内另外2株CanineCV遗传距离较远。本研究首次对国内腹泻犬进行了CanineCV感染的调查,证明CanineCV在四川部分地区宠物犬中已流行,建议把CanineCV作为犬腹泻病原诊断的一个检测内容,同时对其分子特征的研究结果将有助于进一步了解国内CanineCV的遗传多样性。
 
Abstract:
The aim of this study was to investigate the infection and the genomic characteristics of canine circovirus (CanineCV) in part areas of Sichuan during 2015 to 2017. CanineCV infection was detected in 32 diarrheic samples (15.4%) from 6 pet hospitals in Sichuan by real-time PCR, there was no significant difference in the virus prevalence in different years. All CanineCV-positive samples were co-infected with enterovirus of one or more. In addition, six complete CanineCV Rep gene sequences and 1 complete genome of a CanineCV strain (CD17/2016) were sequenced. Phylogenetic analysis showed Sichuan CanineCVs were highly diverse from the other two Chinese CanineCV strains available in the GenBank. This study is the first investigation of CanineCV in dog with diarrhea symptoms in China, indicating that CanineCV is circulating in diarrheic dogs in part areas of Sichuan. This study provided the data of Chinese CanineCV genetic evolution, contributing to understanding of CanineCV genetic diversity.

参考文献/References:

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(本文编辑:李 爽)

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更新日期/Last Update: 2019-04-26