[1]朱利霞?,王洪彬?,赵希艳,等.牛源腔隙莫拉菌的分离鉴定与药敏试验[J].中国预防兽医学报,2019,(03):251-255.[doi:0.3969/j.issn.1008-0589.201807012]
　ZHU Li-xia,WANG Hong-bin,ZHAO Xi-yan,et al.Isolation, identification and drug susceptibility test of bovine Moraxella lacunata[J].Chinese journal of preventive veterinary medicine,2019,(03):251-255.[doi:0.3969/j.issn.1008-0589.201807012]
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牛源腔隙莫拉菌的分离鉴定与药敏试验()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2019年03
页码:
251-255
栏目:
病原生物学
出版日期:
2019-04-25

文章信息/Info

Title:
Isolation, identification and drug susceptibility test of bovine Moraxella lacunata
文章编号:
1008-0589(2019)03-0251-05
作者:
 

朱利霞?; 王洪彬?; 赵希艳; 王双月; 史秋梅*; 高光平*

 (河北科技师范学院  河北省预防兽医学重点实验室，河北 秦皇岛 066600)
Author(s):
 

ZHU Li-xia?;  WANG Hong-bin?;  ZHAO Xi-yan;  WANG Shuang-yue;  SHI Qiu-mei*;  GAO Guang-ping*

 (Hebei key laboratory of preventive veterinary medicine, Hebei Normal University of Science &Technology, Qinhuangdao 066600, China)
关键词:
牛; 腔隙莫拉菌; 分离与鉴定; 药敏试验
Keywords:
cattle;  ;  Moraxella lacunata;  ;  isolation and identification;  ;  drug susceptibility test
分类号:
S852.61
DOI:
0.3969/j.issn.1008-0589.201807012
文献标志码:
A
摘要:
为确定河北省某牛场牛细菌病的病原，本研究从采集的发病牛鼻腔拭子样品中分离到1株细菌，并对其进行常规形态学观察、生化鉴定、16S rDNA基因序列测定、动物致病性试验及药物敏感试验。结果显示，该分离菌株与腔隙莫拉菌的同源性为97.6 %，系统发育进化分析显示其与腔隙莫拉菌位于同一进化分支；该菌株对小白鼠具有一定的致病性，将其命名为Mor QHD-1。西药药敏试验结果显示，分离菌株Mor QHD-1对氨苄西林、环丙沙星、阿米卡星等敏感；对磺胺间甲氧嘧啶、复方新诺明、磺胺二甲氧嘧啶耐药；中药药敏试验结果显示，分离菌株Mor QHD-1对苏木、诃子、乌梅、黄柏、五味子、黄连、五倍子极敏感；苏木、诃子、乌梅、黄柏、五味子、黄连、五倍子对该菌的最小抑菌浓度在1.95 mg/mL～31.25 mg/mL之间，最小杀菌浓度在3.91 mg/mL～62.5 mg/mL之间；本研究在国内首次分离到牛源腔隙莫拉菌，为进一步研究腔隙莫拉菌的致病机制、耐药机制及其跨物种传播奠定了基础。
　　
Abstract:
In order to identify the bacteroidal pathogen from the affected cattle in a cattle farm in Hebei province, a dominant bacteria was isolated from nasal cavity of sick cattle and identified as Moraxella lacunata by using conventional morphological observation, biochemical tests, bacteria 16S rDNA gene sequencing, pathogenicity test and drug sensitive test, demonstrating that the isolate 16S rDNA was 97.6% homologous to M.lacunata, named Mor QHD-1, which had certain pathogenicity to mice. The drug susceptibility tests showed that the isolate of Mor QHD-1 was sensitive to ampicillin, ciprofloxacin, amikacin, etc. but resistance to sulfamonomethoxine, compound sulfamethoxazole and sulfamethoxazole. The results of traditional Chinese medicine susceptibility test showed that the isolate of Mor QHD-1 was extremely sensitive to Caesalpinia sappan, Terminalia chebuLa, Prunus mume, Phellodendron amurense, Schisandra chinensis, Coptis chinensis and Galla chinensis; The minimum inhibitory concentration of Caesalpinia sappan, Terminalia chebuLa, Prunus mume, Phellodendron amurense, Schisandra chinensis, Coptis chinensis and Galla chinensis was ranged from 1.95mg/mL to 31.25mg/mL and the minimum bactericidal concentration of Caesalpinia sappan, Terminalia chebuLa, Prunus mume, Phellodendron amurense, Schisandra chinensis, Coptis chinensis and Galla chinensis was ranged from 3.91mg/mL to 62.5 mg/mL. In this study, a bovine M.lacunata was isolated for the first time in China,and the M.lacunata of Mor QHD-1 would facilitate further study of the pathogen mechanism, resistance mechanism and cross species transmission.

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                                (本文编辑：李　娜)

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更新日期/Last Update: 2019-04-26