[1]张志强,李巧玲,肖丽荣,等.迟缓爱德华菌menF基因缺失株的构建及生物学特性研究[J].中国预防兽医学报,2019,(03):245-250.[doi:0.3969/j.issn.1008-0589.201806020]
 ZHANG Zhi-qiang,LI Qiao-ling,XIAO Li-rong,et al.Construction and characterization of MenF-deficient mutant of Edwardsiella tarda strain[J].Chinese journal of preventive veterinary medicine,2019,(03):245-250.[doi:0.3969/j.issn.1008-0589.201806020]
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迟缓爱德华菌menF基因缺失株的构建及生物学特性研究()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2019年03
页码:
245-250
栏目:
病原生物学
出版日期:
2019-04-25

文章信息/Info

Title:
Construction and characterization of MenF-deficient mutant of  Edwardsiella tarda strain
文章编号:
1008-0589(2019)03-0245-06
作者:
 

张志强1李巧玲1肖丽荣1苏硕青1李永慧2吴同垒1王洪彬1王双月1朱国强3*史秋梅1*

 (1. 河北科技师范学院 河北省预防兽医学重点实验室,河北 秦皇岛 066004;
2. 秦皇岛市第二医院,河北 昌黎 066600;3. 扬州大学,江苏 扬州 225009)
Author(s):
 

ZHANG Zhi-qiang1 LI Qiao-ling1 XIAO Li-rong1 SU Shuo-qing1 LI Yong-hui2 WU Tong-lei1WANG Hong-bin1 WANG Shuang-yue1 ZHU Guo-qiang3* SHI Qiu-mei1*

 (1. Hebei Key Laboratory of Preventive Veterinary Medicine, Hebei Normal University of Science &Technology, Qinhuangdao 066004, China;
2. The Second Hospital of Qinhuangdao, Changli 066600, China; 3. Yangzhou University, Yangzhou 225009, China)
关键词:
迟缓爱德华菌异分支酸合成酶MenF生物学特性
Keywords:
Edwardsiella tarda  isochorismate synthase  MenF  biochemical characteristics
分类号:
S852.61
DOI:
0.3969/j.issn.1008-0589.201806020
文献标志码:
A
摘要:
为研究异分支酸合成酶MenF在迟缓爱德华菌(E.tarda)致病中的作用,本研究利用自杀载体构建了E.tarda menF基因缺失菌株(ET-CLΔmenF),并分析其生物学特性。结果显示,ET-CLΔmenF株比野生菌株生长减缓。体外应激试验结果表明,ET-CLΔmenF菌株对铁饥饿的耐受能力明显下降,应对酸性和过氧化氢处理的能力显著下降。细胞感染实验表明ET-CLΔmenF菌株的胞内存活能力显著下降(p<0.01)。对斑马鱼的致病性结果表明,ET-CLΔmenF株毒力下降。本研究为E.tarda致病机制研究以及疫苗的研制提供了参考。
  
Abstract:
To investigate the role of isochorismate synthase MenF in Edwardsiella tarda, we generated a MenF-knockout mutant with the suicide plasmid pRE112 and characterized the biochemical properties of the mutant. The data showed that, the MenF-knockout strain led to retarded growth in LB and Minimal (M9) medium. Furthermore, the MenF-deficient mutant displayed sharply decreased survival under acidic and H2O2 treatments. The resistance to iron starvation was compromised in the MenF-deficient mutant, compared with the wild type strains. Moreover, MenF-inactivation-caused reduced cellular survival of E.tarda in Raw264.7 cells was determined. In addition, we found that MenF-knockout strain resulted in virulence attenuation in the zebra fish model. In conclusion, this study provided insight into the underlying mechanism of E.tarda infection in aquaculture.

参考文献/References:

[1] Nelson J J, Nelson C A, Carter J E. Extraintestinal manifestations of Edwardsiella tarda infection: A 10-year retrospective review [J]. J La State Med Soc, 2009, 161(2): 103-106.
[2]Hirai Y, Asahata-Tago S, Ainoda Y, et al. Edwardsiella tarda bacteremia. A rare but fatal water- and foodborne infection: Review of the literature and clinical cases from a single centre [J]. Can J Infect Dis Med Microbiol, 2015, 26(6): 313-318.
[3]李杰. 迟缓爱德华氏菌弱毒疫苗的研制和免疫效果[D]. 青岛:中国科学院研究生院(海洋研究所),2012.
[4]Han Xian-gan, Bai Hao, Tu Jian, et al. Deletion of luxS further attenuates the virulence of the avian pathogenic Escherichia coli roA mutant [J]. Microb Pathog, 2015, 88: 39-47.
[5]Matsui H, Isshiki Y, Eguchi M, et al. Evaluation of the live vaccine efficacy of virulence plasmid-cured, and phoP- or aroA-deficient Salmonella enterica serovar Typhimurium in mice [J]. J Vet Med Sci, 2015, 77(2): 181-186.
[6]Yao Zu-jie, Wang Zhi-hong, Sun Lin-an, et al. Quantitative proteomic analysis of cell envelope preparations under iron starvation stress in Aeromonas hydrophila [J]. BMC Microbiol, 2016, 16(1): 161.
[7]Chi Feng, Wang Ying, Gallaher T K, et al. Identification of IbeR as a stationary-phase regulator in meningitic Escherichia coli K1 that carries a loss-of-function mutation in rpoS [J]. J Biomed Biotechnol, 2009, 2009: 520283.
[8]Dong Hong-yan, Peng Da-xin, Jiao Xin-an, et al. Roles of the spiA gene from Salmonella enteritidis in biofilm formation and virulence [J]. Microbiology, 2011, 157(Pt 6): 1798-1805.
[9]孙云. 迟缓爱德华氏菌高效疫苗的设计、构建以及免疫机制研究[D]. 青岛:中国科学院研究生院(海洋研究所),2013.
[10]Buss K, Muller R, Dahm C, et al. Clustering of isochorismate synthase genes menF and entC and channeling of isochorismate in Escherichia coli [J]. Biochim Biophys Acta, 2001, 1522(3): 151-157.
[11]Domagalski M J, Tkaczuk K L, Chruszcz M, et al. Structure of isochorismate synthase DhbC from Bacillus anthracis [J]. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2013, 69(Pt 9): 956-961.
[12]Sridharan S, Howard N, Kerbarh O, et al. Crystal structure of Escherichia coli enterobactin-specific isochorismate synthase (EntC) bound to its reaction product isochorismate: Implications for the enzyme mechanism and differential activity of chorismate-utilizing enzymes [J]. J Mol Biol, 2010, 397(1): 290-300.
[13]Pelludat C, Brem D, Heesemann J. Irp9, encoded by the high-pathogenicity island of Yersinia enterocolitica, is able to convert chorismate into salicylate, the precursor of the sidero- phore yersiniabactin [J]. J Bacteriol,  2003, 185(18): 5648-5653.
[14]Leung K Y, Siame B A, Tenkink B J, et al. Edwardsiella tarda - virulence mechanisms of an emerging gastroenteritis pathogen [J]. Microbes Infect, 2012, 14(1): 26-34.
[15]Mo Zhao-lan, Li Jie, Li Gui-yang, et al. Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda  LSE40 aroA mutant [J]. Appl Microbiol Biotechnol, 2013, 97(14):  6325-6335.
(本文编辑:李 爽)

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更新日期/Last Update: 2019-04-26