[1]王玉建,商红旗,朱 琳,等.产气荚膜梭菌β毒素蛋白抗原表位预测及CPB-N蛋白免疫原性分析[J].中国预防兽医学报,2019,(02):188-191.[doi:0.3969/j.issn.1008-0589.201805006]
 WANG Yu-jian,SHANG Hong-qi,ZHU Lin,et al.Prediction of beta toxin protein epitope of Clostridium perfringens and analysis of immunogenicity of CPB-N protein[J].Chinese journal of preventive veterinary medicine,2019,(02):188-191.[doi:0.3969/j.issn.1008-0589.201805006]
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产气荚膜梭菌β毒素蛋白抗原表位预测及CPB-N蛋白免疫原性分析()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2019年02
页码:
188-191
栏目:
免疫学
出版日期:
2019-02-15

文章信息/Info

Title:
Prediction of beta toxin protein epitope of Clostridium perfringens and analysis of immunogenicity of CPB-N protein
文章编号:
1008-0589(2019)02-0188-04
作者:
 

王玉建1商红旗1朱 琳1徐煜琳1胡莉萍2*朱瑞良1*

 (1. 山东农业大学 动物科技学院/山东省动物生物技术与疫病防治重点实验室,山东 泰安 271018;
2. 山东省动物疫病预防与控制中心,山东 济南 250022) 
Author(s):
 

WANG Yu-jian1 SHANG Hong-qi1 ZHU Lin1 XU Yu-lin1 HU Li-ping2* ZHU Rui-liang1*

 (1. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention/
College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271018, China;
2. Animal disease prevention and control center of Shandong Province, Jinan 250022, China)
 
关键词:
产气荚膜梭菌β毒素蛋白抗原表位免疫原性
Keywords:
 Clostridium perfringens  Beta toxin  antigenic determinant  immunogenicity
分类号:
S852.6
DOI:
0.3969/j.issn.1008-0589.201805006
文献标志码:
A
摘要:
为获得具有与β毒素蛋白相同免疫原性的新蛋白,本研究对产气荚膜梭菌β毒素蛋白的氨基酸序列进行综合分析,预测其抗原表位并筛选出有效的新蛋白。采用生物信息学方法综合分析β毒素蛋白二级结构、亲水性、可塑性、抗原性、跨膜区域以及信号肽,同时参考ElliPro服务器在线预测。最终预测aa108~aa320区段为优势抗原表位,在此基础上建立β毒素蛋白三维结构模型,标记特殊位点并筛选出B型产气荚膜梭菌新蛋白(CPB-N)。诱导表达CPB-N后通过SDS-PAGE和western blot鉴定,结果显示在约24 ku处有明显条带。采用间接 ELISA 方法对免疫小鼠的血清和肠道灌洗液样品进行检测,结果表明CPB-N与β毒素蛋白的免疫原性基本相同。CPB-N蛋白的筛选及预防产气荚膜梭菌引起的疾病奠定了重要的研究基础。
 
Abstract:
In order to identify a new protein with the same immunogenicity as the beta toxin protein, we comprehensively analyzed the amino acid sequence of Clostridium perfringens the beta toxin protein, predicted the epitope and screened for the new protein with the similar epitope. The bioinformatics method was used to comprehensively analyze the secondary structure, hydrophilicity, surface possibility, plasticity, antigenicity, transmembrane region and signal peptide of the beta toxin protein, and refer to online prediction results for ElliPro servers. Finally, we predicted that the amino acid sequence of the aa108-aa320 was the dominant epitope, established a three-dimensional structural model of the beta toxin protein, labeled a specific site and screened the CPB-N protein. After induction of expression of CPB-N, obvious bands about 24ku was observed in the identification of 10% SDS-PAGE and western blot. The serum and intestinal lavage fluid samples from immunized mice were tested by indirect ELISA. The results showed that the immunogenicity of CPB-N were essentially same as the beta toxin protein. The selection and confirmation of CPB-N protein provided an important research basis for the prevention of diseases caused by C.perfringens.

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(本文编辑:李 爽)

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更新日期/Last Update: 2019-03-07