[1]周林宜,张艳萍,孙国荣,等.鸡马立克氏病病毒感染对鸡神经系统损伤的研究[J].中国预防兽医学报,2019,(02):113-117.[doi:0.3969/j.issn.1008-0589.201804020]
　ZHOU Lin-yi,ZHANG Yan-ping,SUN Guo-rong,et al.Study on the injury of chicken nervous system caused by Marek’s disease virus infection[J].Chinese journal of preventive veterinary medicine,2019,(02):113-117.[doi:0.3969/j.issn.1008-0589.201804020]
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鸡马立克氏病病毒感染对鸡神经系统损伤的研究()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2019年02
页码:
113-117
栏目:
病原生物学
出版日期:
2019-02-15

文章信息/Info

Title:
Study on the injury of chicken nervous system caused by Marek’s disease virus infection
文章编号:
1008-0589(2019)02-0113-05
作者:
 

周林宜; 张艳萍; 孙国荣; 张　峰; 于正浩; 高玉龙; 祁小乐; 李　凯; 王笑梅*; 刘长军*

 (中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/禽免疫抑制病创新团队，黑龙江 哈尔滨150069)
Author(s):
 

ZHOU Lin-yi;  ZHANG Yan-ping;  SUN Guo-rong;  ZHANG Feng;  YU Zheng-hao;  GAO Yu-long; QI Xiao-le;  LI Kai;  WANG Xiao-mei*;  LIU Chang-jun*

 (State Key Laboratory of Veterinary Biotechnology/Division of Avian Immunosuppressive Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China)
关键词:
鸡马立克氏病; 神经系统; 病毒载量; 病理损伤
Keywords:
 ; Marek’s disease;  ;  nervous system;  ;  viral load;  ;  pathological damage
分类号:
S852.65
DOI:
0.3969/j.issn.1008-0589.201804020
文献标志码:
A
摘要:
为研究不同毒力的鸡马立克氏病毒(MDV)在鸡神经系统中的感染规律及其对神经系统的损伤进程，本研究选用不同毒力的血清1型MDV (MDV-1)病毒株感染4日龄的SPF鸡，在接毒后1 d、3 d、5 d、7 d、10 d、14 d、17 d、21 d、25 d、28 d和35 d动态检测病毒载量的变化，并对感染后5 d和21 d的不同病毒株感染鸡的脑部和坐骨神经进行组织病理学观察。结果显示，MDV-1强毒株在SPF鸡脑部的复制能力显著高于弱毒株(p<0.05)；特超强病毒株BS在脑组织中出现的时间最早，早期复制最快。但不同毒力的MDV-1株在坐骨神经处的复制能力与其毒力无直接的关系。组织病理学观察显示，在感染早期MDV-1强毒株对SPF鸡脑组织的损伤强于弱毒株；在感染后21 d，强毒株和弱毒株造成的脑部损伤存在明显的不同；而在坐骨神经处，强毒株造成的损伤明显强于弱毒株。本研究揭示了不同MDV病毒株在SPF鸡脑和坐骨神经的复制动力学特征和组织病理学特征，为MDV-1在宿主神经系统中的感染、增殖及造成的损伤提供了实验依据。
Abstract:
Chicken Marek’s disease virus (MDV) is a chicken herpesvirus with neurotropic. MDV infection causes Marek’s disease (MD) and leads to tumors and neurological symptoms in chickens. In order to study the infection regulation and damage process in the chicken nervous system of the different virulent MDV strains, we used different virulent MDV-1 strains to infect 4-day-old SPF chickens. Changes in viral load were detected dynamically at 1, 3, 5, 7, 10, 14, 17, 21, 25, 28 and 35 days after infection, and histopathological observations of brain and sciatic nerve were performed at 5 and 21 days after infection. The results showed that the replication regulation of different virulent MDV-1 strains in the brain was different. The replication ability of virulent strains was significantly higher than that of the attenuated strains. The most virulent strain BS had the fastest replication in the brain at early infection stages and appeared earliest. However, the replication ability of the different virulent MDV-1 strains in the sciatic nerve was not directly related to its virulence. Pathological observations revealed that MDV-1 virulent strains had a stronger damage in brain tissue than attenuated strains at early stage of infection. At 21 days after infection, the brain damage caused by virulent and attenuated strains was significantly different, the damage caused by virulent strains was significantly stronger at the sciatic nerve than that of the attenuated virus. Our study reveals the replicative and histopathological features of different strains in the brain and sciatic nerves and provide the basis for further studies of the neuronal damage caused by MDV-1.

参考文献/References:

[1] Saif Y M. 禽病学[M]. 苏敬良. 北京：中国农业出版社，2005.
[2]Witter R L. Characteristics of Marek’s disease viruses isolated from vaccinated commercial chicken flocks: association of viral pathotype with lymphoma frequency [J]. Avian Dis, 1983, 27(1): 113-132.
[3]itter R L, Gimeno I M, Reed W M, et al. An acute form of transient paralysis induced by highly virulent strains of Marek’s disease virus [J]. Avian Dis, 1999, 43(4): 704-720.
[4]张颖，刘长军，秦运安，等. 应用双重实时荧光定量PCR方法检测鸡马立克氏病血清1型病毒[J]. 中国预防兽医学报, 2007，29(1)：46-51.
[5]Zhang Yan-Ping, Li Zhi-Jie, Bao Ke-Yan, et al. Pathogenic characteristics of Marek’s disease virus field strains prevalent in China and the effectiveness of existing vaccines against them [J]. Vet Microbiol, 2015, 177(1-2): 62-68.
[6]Sun Guo-Rong, Zhang Yan-Ping, Lv Hong-Chao, et al. A Chinese variant Marek’s disease virus strain with divergence between virulence and vaccine resistance [J]. Viruses, 2017, 9(4): E71.
[7]Cho K, Endoh D, Qian J, et al. Central nervous system lesions induced experimentally by a very virulent strain of Marek’s disease virus in Marek’s disease resistant chickens [J]. Avian Pathol, 1998, 27(5): 512-517.
[8]Yunis R, Jarosinski K W, Schat K A, et al. Association between rate of viral genome replication and virulence of Marek’s disease herpesvirus strains [J]. Virology, 2004, 328(1): 142-150.
[9]李晶梅，刘长军，张艳萍，等. 鸡马立克氏病病毒在鸡淋巴细胞和羽髓中的复制动力学分析[J]. 中国预防兽医学报，2008，30(11)：879-884.
[10]Yang Qin, Wei Pan, Chen Hong. Cytokine responses and inducible nitrous oxide synthase expression patterns in neonatal chicken brain microglia infected with very virulent Marek’s disease virus strain YL040920 [J]. Vet Immunol Immunopathol, 2011, 142(1-2): 14-24.
[11]Xu Ming, Fitzgerald S D, Zhang Hai, et al. Very virulent plus strains of MDV induce an acute form of transient paralysis in both susceptible and resistant chicken lines [J]. Viral Immunol, 2012, 25(4): 306-323.
[12]Gimeno I M, Witter R L, Hunt H D, et al. Marek’s disease virus infection in the brain: virus replication, cellular infiltration, and major histocompatibility complex antigen expression [J]. Vet Pathol, 2001, 38(5): 491-503.
                                            (本文编辑：彭永刚)

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更新日期/Last Update: 2019-03-06