[1]华承薇,彭 婕,欧阳雨晴,等. 马链球菌兽疫亚种IdeZ抗体降解酶特性研究[J].中国预防兽医学报,2018,(10):950-955.[doi:10.3969/j.issn.1008-0589.201712035]
 HUA Cheng-wei,PENG Jie,OUYANG Yu-qing,et al. Characteristics of IgG degradation enzyme IdeZ in Streptococcus equi ssp. zooepidimicus[J].Chinese journal of preventive veterinary medicine,2018,(10):950-955.[doi:10.3969/j.issn.1008-0589.201712035]
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马链球菌兽疫亚种IdeZ抗体降解酶特性研究

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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年10
页码:
950-955
栏目:
免疫学
出版日期:
2018-11-15

文章信息/Info

Title:
 

Characteristics of IgG degradation enzyme IdeZ in Streptococcus equi ssp. zooepidimicus

文章编号:
1008-0589(2018)10-0950-06
作者:
 

华承薇彭 婕欧阳雨晴范红结马 喆*

 

(南京农业大学 动物医学院,江苏 南京 210095)

Author(s):
 

HUA Cheng-wei PENG Jie OUYANG Yu-qing FAN Hong-jie MA Zhe*

 

(Nanjing Agricultural University, College of Veterinary Medicine, Jiangsu Province, Nanjing 210095, China)

关键词:
链球菌兽疫亚种IdeZ蛋白抗体降解
Keywords:
 

Streptococcus equi ssp. zooepidimicus IdeZ antibody digestion

分类号:
S852.61
DOI:
10.3969/j.issn.1008-0589.201712035
文献标志码:
A
摘要:
 

本实验室前期研究通过基因组序列比对发现SEZ ATCC 35246株IdeZ基因与化脓链球菌抗体降解蛋白IdeS基因有较高同源性。为进一步了解马链球菌兽疫亚种(SEZ)中IdeZ基因编码蛋白的生物学功能及其可能在细菌抵抗吞噬过程中的作用,本研究利用pCold-SUMO重组系统表达可溶性重组蛋白IdeZ,采用体外模拟抗体降解的试验检测IdeZ蛋白降解IgG抗体的活性。结果显示,在37 ℃条件下,IdeZ蛋白表现出高效的抗体降解活性,体外反应10 min即可用SDS-PAGE检测到IgG降解产物;借助巨噬细胞Raw264.7细胞模型,体外模拟巨噬细胞吞噬SEZ试验检测IdeZ蛋白对抗体介导的巨噬细胞吞噬SEZ活性的影响,结果显示,IdeZ蛋白可有效抑制SEZ免疫小鼠血清或其纯化的IgG的吞噬功能。表明IdeZ蛋白有助于 SEZ抵抗巨噬细胞的吞噬功能。本研究为进一步揭示SEZ抵抗巨噬细胞吞噬机制提供了新的切入点。

  

Abstract:
 

Streptococcus equi ssp. zooepidimicus (SEZ) has the ability of anti-phagocytosis. This pathogen causes swine streptococcosis. SEZ ATCC35246 IdeZ gene has high homology with the IgG-degrading enzyme of Streptococcus pyogene (IdeS) gene. In present study, we expressed and purified the recombinant IdeZ protein through His trap and molecular sieve chromatography. In vitro test results showed that the IdeZ protein was able to digest IgG at 37℃ within 10 min after incubation with IgG as 1∶100 (m/m). With the Raw264.7 macrophage cell model, we found that IdeZ decreased the oposonization of anti-SEZ serum or its purified IgG and suppressed the phagocytosis of macrophage to SEZ. This study provides valuable reference in illustrating the anti-phagocytosis mechanism based on antibody digestion in SEZ.

参考文献/References:

 

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(本文编辑:李 娜)



更新日期/Last Update: 2018-11-28