[1]李乙江,张泉鹏,苗海生,等. 口蹄疫灭活病毒配合枯草芽孢杆菌对提高牛呼吸道先天免疫力的研究[J].中国预防兽医学报,2018,(10):934-939.[doi:10.3969/j.issn.1008-0589.201801008]
 LI Yi-jiang,ZHANG Quan-peng,MIAO Hai-sheng,et al. Intranasal immunization with inactivated foot-and-mouth disease virus and Bacillus subtilis improve innate immunity of cattle respiratory tract mucosa[J].Chinese journal of preventive veterinary medicine,2018,(10):934-939.[doi:10.3969/j.issn.1008-0589.201801008]
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口蹄疫灭活病毒配合枯草芽孢杆菌对提高牛呼吸道先天免疫力的研究

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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年10
页码:
934-939
栏目:
免疫学
出版日期:
2018-11-15

文章信息/Info

Title:
 

Intranasal immunization with inactivated foot-and-mouth disease virus and Bacillus subtilis improve innate immunity of cattle respiratory tract mucosa

文章编号:
1008-0589(2018)10-0934-06
作者:
 

李乙江12张泉鹏3苗海生4段体祥5张应国6杨柱昌7李世钰8冯德正9陈德寿10

雷锦文11高花英1鲍晓伟4李作生12杨 倩2*

 

(1. 德宏州动物疫病预防控制中心,云南 德宏 678400;2. 南京农业大学 动物医学院,江苏 南京 210095;3. 昆明市动物卫生监督所,云南 昆明 650000;4. 云南省畜牧兽医科学院,云南 昆明 650000;5. 风平镇农业综合服务中心,云南 德宏 678400;

6. 景罕镇镇农业综合服务中心,云南 德宏 678700;7. 芒市动物疫病预防控制中心,云南 德宏 678400;8. 云南大学 农学院,

云南 昆明 650500;9. 陇川县动物疫病预防控制中心,云南 德宏 678700;10. 新城乡农业综合服务中心,云南 德宏 679300;

11. 盏西镇农业综合服务中心,云南 德宏 679300;12. 武汉中博生物股份有限公司,湖北 武汉 430000)

Author(s):
 

LI Yi-jiang12 ZHANG Quan-peng3 MIAO Hai-sheng4 DUAN Ti-xiang5 ZHANG Ying-guo6

YANG Zhu-chang7 LI Shi-yu8 FENG De-zheng9 CHEN De-shou10 LEI Jin-wen11 GAO Hua-ying1

BAO Xiao-wei4 LI Zuo-sheng12 YANG Qian2*

 

(1. Dehong Animal Disease Control Center, Dehong 678400, China; 2. Animal Medicine College, Nanjing Agricultural University, Nanjing 210095, China; 3. Kunming Animal Health Inspection, Kunming 650000, China; 4. Yunnan Animal Sience and Veterinary Institute, Kunming 650000, China; 5. Agricultural Comprehensive Service Center of Fengpin Town, Dehong 678400, China; 6. Agricultural Comprehensive Service Center of Jinghan Town, Dehong 678700, China; 7. Mangshi County Animal Disease Control Center, Dehong 678400, China; 8. School of Agriculture Yunnan University, Yunnan University, Kunming 650500, China; 9. Longchuan County Animal Disease Control Center, Dehong 678700, China; 10. Agricultural Comprehensive Service Center of Xinchen Town, Dehong 679300, China; 11. Agricultural Comprehensive Service Center of Zanxi Town, Dehong 679300, China; 12. Wuhan Chopper Biology CO., LTD, Wuhan 430000, China)

关键词:
O型口蹄疫灭活病毒枯草芽孢杆菌鼻腔免疫呼吸道黏膜TLR7CD3+淋巴细胞细胞因子
Keywords:
 

inactivated foot-and-mouth disease virus bacillus subtilis intranasal immunization respiratory tract mucosa TLR7 CD3+ lymphocytes cytokine

分类号:
S852.65
DOI:
10.3969/j.issn.1008-0589.201801008
文献标志码:
A
摘要:
 

本研究采用O型口蹄疫灭活病毒配合枯草芽孢杆菌喷鼻气雾免疫健康牛,并设单独使用枯草芽孢杆菌组、灭活病毒、常规疫苗免疫组和空白对照组,评价O型口蹄疫灭活病毒配合免疫增强剂枯草芽孢杆菌通过喷鼻气雾免疫健康牛对其呼吸道先天免疫力的影响。通过免疫组织化学染色显示,免疫后灭活病毒配合免疫增强剂试验组牛鼻黏膜与肺内支气管黏膜中TLR7的表达和CD3+淋巴细胞呈极显著增多(p<0.01);而常规口蹄疫灭活苗对其TLR7的表达和CD3+淋巴细胞数量的影响不显著(p>0.05)。ELISA检测结果显示,与对照组相比,灭活病毒配合免疫增强剂试验组牛呼吸道组织中IL-12水平呈极显著增加(p<0.01),IL-4和IL-10水平无明显差异(p>0.05);而常规口蹄疫灭活苗对牛呼吸道组织中IL-4、IL-10和IL-12的影响不明显(p>0.05)。表明O型口蹄疫灭活病毒配合枯草芽孢杆菌能够提高牛呼吸道先天免疫力。本研究为研发口蹄疫灭活病毒的鼻腔疫苗提供重要的试验数据和参考。

  

Abstract:
 

To explore the influence of innate immunity on cattle respiratory tract after intranasal aerosol immunized with inactivated foot-and-mouth disease virus (FMDV) along with Bacillus subtilis. In this study, we used inactivated FMDV along with B.subtilis to immune cattle by intranasal aerosol, and set up control group including single use of B.subtilis, inactivated FMDV,conventional inactivated FMDV vaccine and negative control. The results of immunohistochemistry assay showed that expressing TLR7 and CD3+ lymphocytes were significantly increased in cattle nasal mucosae and pulmonary bronchial (p<0.01) after intranasal immunized with inactivated FMDV along with B.subtilis, but there was no significant influences (p>0.05) in the cattle only immunized with conventional inactivated FMDV vaccine compared with other control group. At the same time, the results of ELISA showed that the level of IL-12 was significantly increased (p<0.01, p<0.05) and the level of IL-4 and IL-10 were not significant (p>0.05) in cattle respiratory tract tissues after intranasal immunized with inactivated FMDV along with B.subtilis, included nasal, tracheal, pulmonary and pulmonary bronchial. However, the level of IL-4, IL-10 and IL-12 were not significant (p>0.05) in cattle respiratory tract tissues after the inactivated FMDV vaccine immunization. These results indicated that innate immunity of the cattle respiratory tract were improved by intranasal immunization of inactivated FMDV along with B.subtilis. Our study provided important experimental data and reference for the development of the inactivated FMDV vaccine via intranasal aerosol immunization.

参考文献/References:

 

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(本文编辑:李 娜)

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更新日期/Last Update: 2018-11-28