[1]潘俊慧,梁真洁,陈普成,等. Clade 2.3.2e H5亚型禽流感DNA疫苗的免疫保护效力研究[J].中国预防兽医学报,2018,(10):930-933.[doi:10.3969/j.issn.1008-0589.201712002]
 PAN Jun-hui,LIANG Zhen-jie,CHEN Pu-cheng,et al. Protective efficacy of DNA vaccine against clade 2.3.2e H5N1 avian influenza virus[J].Chinese journal of preventive veterinary medicine,2018,(10):930-933.[doi:10.3969/j.issn.1008-0589.201712002]
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Clade 2.3.2e H5亚型禽流感DNA疫苗的免疫保护效力研究

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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年10
页码:
930-933
栏目:
免疫学
出版日期:
2018-11-15

文章信息/Info

Title:
 

Protective efficacy of DNA vaccine against clade 2.3.2e H5N1 avian influenza virus

文章编号:
1008-0589(2018)10-0930-04
作者:
 

潘俊慧梁真洁陈普成唐 猛曾显营柳金雄邓国华姜永萍*陈化兰*

 

(中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室,哈尔滨 黑龙江 150069)



Author(s):
 

PAN Jun-hui LIANG Zhen-jie CHEN Pu-cheng TANG Meng ZENG Xian-ying LIU Jin-xiong

DENG Guo-hua JIANG Yong-ping* CHEN Hua-lan*

 

(National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin 150069, China)

关键词:
DNA疫苗Clade.2.3.2e禽流感病毒免疫效力评价
Keywords:
 

DNA vaccine clade 2.3.2 avian influenza virus protective efficacy

分类号:
S852.65
DOI:
10.3969/j.issn.1008-0589.201712002
文献标志码:
A
摘要:
 

为研制和更新针对H5亚型禽流感病毒(AIV)流行株的DNA疫苗,本研究将新近分离的H5亚型AIV Clade2.3.2e分支代表株A/Duck/Anhui/S1246/2015 (DK/AH/S1246/15)密码子优化的HA基因定向克隆至载体pCAGGS中构建重组质粒 pCA-S1246,将该质粒转染293T细胞。利用间接免疫荧光和western blot检测,结果显示,HA蛋白可以在293T细胞中正确表达。将15 μg、30 μg和60 μg的pCA-S1246质粒分别免疫3周龄的SPF鸡,3周以后以相同的剂量加强免疫后1周,检测其HI抗体平均效价分别可达1∶56、1∶16和1∶37;加强免疫1周后用105 EID50的DK/AH/S1246/15进行攻击时,免疫组的保护率为100 %。本研究为DNA质粒pCA-S1246作为防控Clade.2.3.2e AIV的候选DNA疫苗株提供了实验依据。

  

Abstract:
 

The constant mutation of hemagglutinin (HA) gene of highly pathogenic avian influenza virus (HPAIV), resulting in antigenic variation, compromises the vaccine immune effects, and there is a pressing need to reconstruct and update candidate vaccine strain according to the HPAIV epidemiological results. In this study, the codon optimized HA gene of latest isolated A/Duck/Anhui/S1246/2015 (DK/AH/S1246/15) (H5N1), a representative strain of clade 2.3.2e HPAIVs, was subcloned into pCAGGS vector to construct the recombinant plasmid pCA-S1246 as the DNA vaccine against AIV. After being transfected into 293T cells, the HA expressed effectively detected by IFA and western blot. In addition, groups of SPF chickens were immunized with the doses of 15μg, 30μg and 60μg of pCA-S1246, respectively, and boosted with same dose after three weeks to evaluate the protective efficacy of the candidate DNA vaccine. The blood samples were collected from the immunized chickens in each dose group for the antibody detection, and the average HI titers were 1:56 of 15μg, 1∶16 of 30μg and 1∶37 of 60μg, respectively, 1 week after boost. Our results showed that pCA-S1246 was able to protect chicken from being infected by DK/AH/S1246/15 completely, and the pCA-S1246 has the potential to be a vaccine candidate against clade 2.3.2e H5N1 virus.

参考文献/References:

 

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(本文编辑:彭永刚)

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更新日期/Last Update: 2018-11-28