[1]李昌红,温贵兰*,张升波,等.从江香猪EDC3基因的克隆及其在不同组织中表达的分析[J].中国预防兽医学报,2018,(09):828-833.[doi:0.3969/j.issn.1008-0589.201803030]
 LI Chang-hong,WEN Gui-lan*,ZHANG Sheng-bo,et al. Cloning and expressing distribution analysis of EDC3 gene in different tissues of Congjiangxiang pig[J].Chinese journal of preventive veterinary medicine,2018,(09):828-833.[doi:0.3969/j.issn.1008-0589.201803030]
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从江香猪EDC3基因的克隆及其在不同组织中表达的分析()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年09
页码:
828-833
栏目:
免疫学
出版日期:
2018-09-17

文章信息/Info

Title:
 

Cloning and expressing distribution analysis of EDC3 gene in different tissues of Congjiangxiang pig

文章编号:
1008-0589(2018)9-0828-06
作者:
 

李昌红12温贵兰12*张升波12徐 丽12林汉卿12田 浪12杨佰启12管国丹12汪德生12

(1. 贵州大学 动物科学学院,贵州 贵阳 550025;2. 贵州省动物疫病与兽医公共卫生重点实验室,贵州 贵阳 550025)
Author(s):
 

LI Chang-hong12 WEN Gui-lan12* ZHANG Sheng-bo12 XU Li12 LIN Han-qing12 TIAN Lang12 YANG Bai-qi12 GUAN Guo-dan12 WANG De-sheng12

  (1. College of Animal Science,Guizhou University, Guiyang 550025, China;
2. Key Laboratory of Animal Health and Veterinary Public Health of Guizhou Province, Guiyang 550025, China)
关键词:
从江香猪EDC3基因克隆组织分布
Keywords:
  Congjiangxiang pig  EDC3 gene  cloning  tissue distribution of the expression
分类号:
S852.4
DOI:
0.3969/j.issn.1008-0589.201803030
文献标志码:
A
摘要:
  :为获得从江香猪源mRNA脱帽增强因子3 (EDC3)基因编码区序列,并分析其在从江香猪器官组织和淋巴组织中的表达情况,本研究利用套式PCR扩增获得EDC3全基因序列,对其进行测序分析;同时建立荧光定量PCR(qPCR)方法,分析EDC3在组织中的表达情况。结果显示,从江香猪源EDC3基因全长1 404 bp,编码468 aa。不同物种间EDC3基因核苷酸序列和推导氨基酸序列具有较高的保守性,该蛋白中存在丰富的磷酸化位点,空间结构以无规则卷曲和α-螺旋为主。qPCR结果显示,EDC3基因在从江香猪不同器官组织及淋巴组织中均有分布且有不同程度的表达,在淋巴组织表达量最高,肺脏中表达量最低。综上,本研究首次克隆出猪源EDC3基因,且与预测野猪EDC3基因编码区序列相比缺失123 bp,在第100、473、819、1252位出现了单个碱基置换;其在不同组织中广泛分布。研究结果为进一步探究哺乳动物源EDC3的作用机制奠定基础。
  
Abstract:
 In order to clone the enhancer of mRNA decapping 3 (EDC3) gene and analyze its expressing in different tissues of Congjiangxiang pig, the EDC3 gene was amplified by nested PCR, cloned and sequenced for bioinformatics analysis. In addition, a real-time PCR method was established to analyze the expressing distribution of the EDC3 in different tissues of the pig. The results indicated that full length of the EDC3 gene was 1,404bp and encoded 468 amino acids. EDC3 gene sequence and amino acid sequence were highly conserved among different species, which contained the abundant phosphorylation sites and assembled with irregular coiling and α-helix. The results of the mRNA detection showed that the expression of EDC3 was distributed in different tissues of the pigs, but the expressed levels were different. The expression of EDC3 was the highest in lymphoid tissues and the lowest in lung tissues. In summary, this study cloned the Congjiangxiangpig EDC3 gene for the first time, which lacked 123 bp in coding region compared with the sequence of the predicted EDC3 gene, and a single base substitutions occurred at the positions of 100bp, 473bp, 819bp and 1,252bp, respectively. The results of the study laid the foundation for further exploring the function of EDC3.

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(本文编辑:李    爽)

更新日期/Last Update: 2018-10-19