[1]姜志刚,徐和敏,邵 葳,等.牛荚膜A型多杀性巴氏杆菌免疫攻毒模型的建立[J].中国预防兽医学报,2018,(09):790-794.[doi:0.3969/j.issn.1008-0589.201711044]
 JIANG Zhi-gang,XU He-min,SHAO Wei,et al.Establishment of vaccination-challenge models for bovine Pasteurella multocida capsular type A[J].Chinese journal of preventive veterinary medicine,2018,(09):790-794.[doi:0.3969/j.issn.1008-0589.201711044]
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牛荚膜A型多杀性巴氏杆菌免疫攻毒模型的建立()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年09
页码:
790-794
栏目:
病原生物学
出版日期:
2018-09-17

文章信息/Info

Title:
Establishment of vaccination-challenge models for bovine Pasteurella multocida capsular type A
文章编号:
1008-0589(2018)9-0790-05
作者:
 

姜志刚1徐和敏2邵 葳2潘春刚2于 力1*

 (1. 中国农业科学院哈尔滨兽医研究所 牛羊传染病研究创新团队,黑龙江 哈尔滨 150069;
2. 中牧实业股份有限公司,北京 100070)
Author(s):
 

JIANG Zhi-gang1 XU He-min2 SHAO Wei2 PAN Chun-gang2 YU Li1*

 (1. Division of Livestock Infections Disease, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China; 2. China Animal Husbandry Industry Co., Ltd., Beijing 100070, China)
关键词:
荚膜A型Pm牛纤维素性化脓性肺炎免疫攻毒模型
Keywords:
 Pasteurella multocida capsular type A  fibrinosuppurative bronchopneumonia  vaccination-challenge model
分类号:
S852.61
DOI:
0.3969/j.issn.1008-0589.201711044
文献标志码:
A
摘要:
牛荚膜A型多杀性巴氏杆菌(Pm)是典型的条件致病菌,为建立该病原菌的免疫攻毒模型,本研究采用不同代次、不同剂量的Pm-TJ株接种实验动物小鼠和本动物牛,测定其对小鼠的最小致死剂量以及对牛的最小感染剂量;在此基础上,将制备的全菌体灭活疫苗分别免疫小鼠和牛,通过攻毒保护试验评价该疫苗对动物的免疫原性。攻毒试验结果显示,Pm-TJ株对小鼠高度致死,最小致死剂量为10 cfu,而且不同代次细菌的毒力无显著差异,表明其在体外传代过程中毒力保持稳定;能够引起Pm-TJ株接种牛典型的纤维素性化脓性肺炎,而且接种牛的发病率呈现剂量依赖性,最小感染剂量为2.5×108 cfu。免疫后攻毒试验结果显示,将含有1×107 cfu灭活菌体的疫苗接种小鼠,其能够抵抗致死剂量细菌的攻击;用含有7.5×109 cfu灭活菌体的疫苗接种犊牛,用两倍最小感染剂量细菌攻击,免疫牛能够获得有效保护。本研究利用实验动物小鼠和本动物牛建立了牛荚膜A型Pm的免疫攻毒模型,并表明该全菌体灭活疫苗具有良好的免疫原性,这些研究结果为Pm疫苗的研发奠定了实验基础。
  
Abstract:
Pasteurella multocida capsular type A is a typical opportunistic pathogen for cattle, the biggest challenge in vaccine development is to establish a vaccination-challenge model. In this study, mice or calves were inoculated with different passages or different doses of the P.multocida capsular type A to determine the minimum lethal dose (MLD) for mouse and the minimum infective dose (MID) for calf. Additionally, the immunogenicity of the bacteria inactivated vaccine was evaluated by vaccination and challenge tests in mice and calves. Challenge tests showed that the Pm-TJ strain exhibited high lethality to mouse with 10 cfu of MLD, and different passages of the bacteria had no significant changes in virulence, indicating that the bacteria kept stability of virulence in a serial passages. Typical fibrinosuppurative bronchopneumonia was reproduced in calves by artificial inoculation of the Pm-TJ strain with a dose-dependent manner in morbidity, and the measured MID was 2.5×108cfu. Vaccination-challenge tests showed that the whole bacteria inactivated vaccine (containing 1×107cfu bacteria) conferred mice protection against lethal dose of the bacteria challenge, and the calves immunized with the vaccine (containing 7.5×109cfu of bacteria) also received effective protection against challenge with 2 MID of the bacteria, indicating that the vaccine exhibited promising immunogenicity in mice and calves. This study provides  an experimental basis for vaccine development of bovine P.multocida capsular type A.

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                           (本文编辑:彭永刚)

相似文献/References:

[1]姜志刚,徐和敏,邵 葳,等.荚膜A型多杀性巴氏杆菌引起牛纤维素性化脓性肺炎:病原引起疾病因果关系的确定[J].中国预防兽医学报,2017,(12):963.[doi:10.3969/j.issn.1008-0589.201707022]
 JIANG Zhi-gang,XU He-min,SHAO Wei,et al.Bovine fibrino suppurative broncho pneumoniais caused by Pasteurella multocida capsular type A: determination of the cause-and-effect relationship between the pathogen and the disease[J].Chinese journal of preventive veterinary medicine,2017,(09):963.[doi:10.3969/j.issn.1008-0589.201707022]

更新日期/Last Update: 2018-09-28