[1]张志强,杨 楠,苏硕青,等.鸡白痢沙门菌htrA基因缺失株的构建及其相关生物学特性研究[J].中国预防兽医学报,2018,(09):785-789.[doi:0.3969/j.issn.1008-0589.201712016]
 ZHANG Zhi-qiang,YANG Nan,SU Shuo-qing,et al. Construction and biological feature identification of htrA knock-out mutant of Salmonella pullorum[J].Chinese journal of preventive veterinary medicine,2018,(09):785-789.[doi:0.3969/j.issn.1008-0589.201712016]
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鸡白痢沙门菌htrA基因缺失株的构建及其
相关生物学特性研究
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年09
页码:
785-789
栏目:
病原生物学
出版日期:
2018-09-17

文章信息/Info

Title:
 Construction and biological feature identification of htrA knock-out mutant of Salmonella pullorum
文章编号:
1008-0589(2018)9-0785-05
作者:
 

张志强134杨 楠4苏硕青4李永慧2黄翠琴3段滇宁3吴同垒4李佩国4*朱国强1*

 (1. 扬州大学,江苏 扬州 225009;2. 秦皇岛第二医院,河北 秦皇岛 066600;3. 福建省家畜传染病防治与生物技术重点实验室,
福建 龙岩 364000;4. 河北科技师范学院,河北 秦皇岛 066004)
Author(s):

 

ZHANG Zhi-qiang134 YANG Nan4 SU Shuo-qing4 LI Yong-hui2 HUANG Cui-qin3 DUAN Dian-ning3 WU Tong-lei4 LI Pei-guo4* ZHU Guo-qiang1*

  (1. Yangzhou University, Yangzhou 225009, China; 2. The second hospital of Qinhuangdao, Qinhuangdao 066600, China;
3. Fujian key laboratory of Prevention and control of livestock contagious disease &biotechnology, Longyan 364000, China;
4. Hebei Normal University of Science &Technology, Qinhuangdao 066604, China)
关键词:
 鸡白痢沙门菌基因缺失HtrA生物学特性
Keywords:
 Salmonella pullorum  knockout  HtrA  biological characteristics
分类号:
S852.61
DOI:
0.3969/j.issn.1008-0589.201712016
文献标志码:
A
摘要:
为研究丝氨酸蛋白酶HtrA对鸡白痢沙门菌在巨噬细胞内生存的影响,本研究利用 λ-Red 同源重组系统构建了鸡白痢沙门菌(ATCC19945) htra基因缺失突变株(ATCC19945Δhtra),通过测定其巨噬细胞内存活能力、应激条件实验,探究HtrA蛋白在鸡白痢沙门菌致病过程中的作用。结果显示,缺失株ATCC19945Δhtra与野生型菌株和回复菌株相比,其生长速度、生化特性无明显差异,但其巨噬细胞内存活能力显著降低。应激环境试验结果表明,htra基因缺失后鸡白痢沙门菌对pH、热和H2O2氧化应激的敏感性增加,在蛋清中生存能力下降,生物被膜形成能力显著下降。本研究为进一步阐释鸡白痢沙门菌的胞内存活机制、疫苗开发奠定基础。
  
Abstract:
To investigate the roles of HtrA protein played for intracellular survival of Salmonella in macrophage, the htrA gene deleted Salmonella pullorum was constructed with λ-Red homologous recombination, followed by further assay of the bacterial intracellular survival in macrophage, biochemical characteristics and stress response. The results showed that HtrA knocked-out had no impact on growth and biochemical characteristics of S.pullorum strain ATCC 19945. While compared to the parent strain, the HtrA knock-out mutant displayed sharply impaired survival in macrophage and the persistence to acidic, alkalic, overheated and H2O2 treament. Moreover, the HtrA knock-out mutant reduced the ablities of biofilm formation and survival in egg albumen. In this study, a HtrA knock-out mutant of S.pullorum was successfully generated, providing a guidance and foundation for mechanism investigation of S.pullorum intracellular infection and vaccine development.

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                                          (本文编辑:彭永刚)

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更新日期/Last Update: 2018-09-28