[1]高俊峰,张 莹,王丽坤,等.乌裕尔河流域富裕段麦穗鱼感染的吸虫囊蚴分子生物学调查[J].中国预防兽医学报,2018,(07):646-649.[doi:10.3969/j.issn.1008-0589.201708021]
 GAO Jun-feng,ZHANG Ying,WANG Li-kun,et al.Survey on metacercariae infection in Pseudorasbora parva by molecular biology method in Fuyu region of Wuyuer river basin[J].Chinese journal of preventive veterinary medicine,2018,(07):646-649.[doi:10.3969/j.issn.1008-0589.201708021]
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乌裕尔河流域富裕段麦穗鱼感染的吸虫囊蚴分子生物学调查()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年07
页码:
646-649
栏目:
研究简报
出版日期:
2018-08-15

文章信息/Info

Title:
Survey on metacercariae infection in Pseudorasbora parva by molecular biology method in Fuyu region of Wuyuer river basin
文章编号:
1008-0589(2018)7-0646-04
作者:
 

高俊峰12张 莹2王丽坤2崔宇超2侯美如2路义鑫1*

 (1. 东北农业大学 农业部动物疫病病原生物学重点实验室/东北科学观测实验站,黑龙江 哈尔滨 150030;
2. 黑龙江省兽医科学研究所 寄生虫研究室,黑龙江 齐齐哈尔 161000)
Author(s):
 

GAO Jun-feng12 ZHANG Ying2 WANG Li-kun2 CUI Yu-chao2 HOU Mei-ru2 LU Yi-xin1*

 (1. Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeast Agricultural University, Harbin 150030, China; 2. Department of Parasitology, Heilongjiang Institute of Veterinary Science, Qiqihar 161000, China)
关键词:
乌裕尔河流域麦穗鱼吸虫囊蚴形态学分子生物学
Keywords:
  Wuyuer river basin  Pseudorasbora parva  metacercariae  morphology  molecular biology
分类号:
S852.7
DOI:
10.3969/j.issn.1008-0589.201708021
文献标志码:
A
摘要:
为了解乌裕尔河流域富裕段麦穗鱼感染吸虫囊蚴的种类,本研究采集乌裕尔河流域富裕段麦穗鱼,利用人工消化法收集的不同形态的吸虫囊蚴,在显微镜下观察,根据囊蚴的形态特点进行分类。同时分别提取各种囊蚴的总DNA,利用PCR方法扩增其第二内转录间隔区(ITS2)基因序列并进行测序,将获得的基因序列与GenBank数据库相应的基因序列进行比对分析,构建系统发生树。结果显示乌裕尔河流域富裕段麦穗鱼主要感染3种吸虫囊蚴,分别为华支睾吸虫、东方次睾吸虫和日本全冠吸虫。本研究进一步证明形态学结合分子生物方法是鉴定淡水鱼中吸虫囊蚴感染种类的有效手段。
  
Abstract:
In order to investigate the kinds of the trematode metacercariae infected Pseudorasbora parva in Fuyu region of Wuyuer river basin, P.parva were collected in this region and the trematode metacercariae were isolated using artificial digestion method. The trematode metacercariae were classified by morphological characteristics utilizing the microscope. The total DNA of each kind of metacercariae was extracted, and the second internal transcriptional space (ITS2) sequences were amplified by PCR and sequenced. Sequences were assembled and aligned with the sequences available in GenBank database, and the phylogenetic relationships were reconstructed based on ITS2 sequences with MP methods. The results showed that three kinds of metacercariae were identified in P.parva, named Clonorchis sinensis, Metorchis orientalis and Holostephanus dubinini, respectively. It is suggested that morphology combined with molecular biological methods is an effective method for identification of metacercariae in freshwater fish.

参考文献/References:

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(本文编辑:李 爽)

更新日期/Last Update: 2018-08-15