[1]张立媛,张紫璇,李卓昕,等.犬细小病毒YBYJ株体外诱导MDCK宿主细胞凋亡的研究[J].中国预防兽医学报,2018,(07):630-633.[doi:0.3969/j.issn.1008-0589.201711013]
 ZHANG Li-yuan,ZHANG Zi-xuan,LI Zhuo-xin,et al.Study on apoptosis of MDCK host cells induced by?canine parvovirus YBYJ strain in vitro[J].Chinese journal of preventive veterinary medicine,2018,(07):630-633.[doi:0.3969/j.issn.1008-0589.201711013]
点击复制

犬细小病毒YBYJ株体外诱导MDCK宿主细胞凋亡的研究()
分享到:

《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年07
页码:
630-633
栏目:
出版日期:
2018-08-15

文章信息/Info

Title:
Study on apoptosis of MDCK host cells induced by?canine parvovirus YBYJ strain in vitro
文章编号:
1008-0589(2018)7-0630-04
作者:
张立媛张紫璇李卓昕王美琪刘晋宇高 旭*鲁 承
?(延边大学 农学院/动物医学系,吉林 延吉 133400)
Author(s):
ZHANG Li-yuan ZHANG Zi-xuan LI Zhuo-xin WANG Mei-qi LIU Jin-yu GAO Xu* LU Cheng
?(Department of Veterinary Medicine, Agricultural College, Yanbian University, Yanji 133400, China)
关键词:
犬细小病毒犬肾细胞细胞凋亡
Keywords:
canine parvovirus? MDCK cells? cell apoptosis
分类号:
S852.65
DOI:
0.3969/j.issn.1008-0589.201711013
文献标志码:
A
摘要:
为研究犬细小病毒(CPV)在体外能否诱导MDCK宿主细胞凋亡,本研究应用前期分离到的CPV YBYJ强毒株接种MDCK细胞,通过倒置显微镜观察CPV导致MDCK宿主细胞病变(CPE)情况,采用细胞凋亡DNA Ladder抽提试剂盒和Annexin V-FITC/PI双染细胞凋亡检测试剂盒和Caspase-3活性检测试剂盒检测,观察CPV诱导MDCK宿主细胞凋亡情况。结果显示,CPV YBYJ强毒株可导致MDCK宿主细胞产生明显CPE,并出现明显的DNA Ladder,Annexin V-FITC/PI双染为阳性,Caspase-3活性显著升高,提示CPV在体外能够诱导MDCK宿主细胞凋亡。
Abstract:
In order to study the effects of canine parvovirus (CPV) inducing apoptosis in MDCK host cells in vitro, MDCK cells were inoculated with virulent CPV YBYJ strain isolated from the previous study, which induced cytopathic effect (CPE) in MDCK cells. Then the apoptosis in the virus infected MDCK cells was examined by ladder DNA extraction kit, Annexin V-FITC/PI double staining cell apoptosis detection kit and Caspase-3 activity detection kit. The results showed that CPV was able to cause the MDCK host cell to appear obvious ladder DNA, positive for Annexin V-FITC/PI double staining, significant increase of Caspase-3 activity. It suggested that CPV had the ability to induce the apoptosis of MDCK host cells in vitro.

参考文献/References:

[1] Hoelzer K, Parrish C R. The emergence of parvoviruses of carnivores [J]. Vet Res, 2010, 41(6): 39-51.
[2]Truyen U, Parrish C R. Canine and feline host ranges of canine parvovirus and feline panleukopenia virus: Distinct host cell tropisms of each virus in vitro and in vivo [J]. J Virol, 1992, 66(9): 5399-5408.
[3]Saxena L, Kumar G R, Saxena S, et al. Apoptosis induced by NS1 gene of canine parvovirus-2 is caspase dependent and p53 independent [J]. Virus Res, 2013, 173(2): 426-430.
[4]潘红丽,仲飞,潘素敏, 等.犬细小病毒NS1非结构蛋白可诱导细胞凋亡[J]. 微生物学报,2012,52(3):367-373.
[5]Nykky J, Tuusa J E, Kirjavainen S, et al. Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine [J]. Int J Nano- medicine, 2010, 5: 417-428.
[6]Pelletier G, Padhi B K. Cyclooxygenase 1 mRNA expression is undetectable in madin darby canine kidney cells [J]. BMC Res Notes, 2015, 24: 88-93.
[7]张立媛,高旭,陈海迪,等. 犬细小病毒YBYJ株的分离鉴定及VP2基因的序列分析[J]. 安徽农业科学,2014,42(27):9370-9372,9401.
[8]赵建军,闫喜军,吴威. 犬细小病毒:从起源到进化[J]. 微生物学报,2011,51(7):869-875.
[9]金丽兰,高旭,张立媛,等. 犬细小病毒及其诱导细胞凋亡的研究进展[J].吉林畜牧兽医,2016,9:11-13.
[10]张立媛,高旭,鲁承,等. 犬细小病毒YBYJ株NS1基因的克隆及序列分析[J]. 黑龙江畜牧兽医,2015,23(12):173-175.
[11]彭晓兰,董晓凯,廖芳芳,等. 马兜铃酸Ⅰ诱导肾小管上皮细胞凋亡的检测[J]. 中国畜牧兽医,2011,38(11):61-65.
[12]张伟,梁智辉. Annexin V-FITC/PI双标记与Hoechst33342/PI双标记流式细胞术检测细胞凋亡的比较[J]. 细胞与分子免疫学杂志,2011,30(11):1209-1212.
[13]徐华. 茶多酚对心脏骤停/心肺复苏大鼠模型脑组织SOD表达及Caspase3/TUNEL阳性的影响[J]. 临床和实验医学杂志,2017,16(8):738-741.
[14]Nykky J, Tuusa J E, Kirjavainen S, et al. Mechanisms of cell death in canine parvovirus infected cells provide intuitive insights to developing nanotools for medicine [J]. Int J Nano- medicine, 2010, 9(5): 417-428.
(本文编辑:李 爽)

相似文献/References:

[1]姜 骞,李慕瑶,刘家森,等.犬细小病毒ELISA抗体检测试剂盒的研制及应用[J].中国预防兽医学报,2009,(02):141.
 JIANG Qian,LI Mu-yao,LIU Jia-sen,et al.Development and application of an indirect-ELISA kit for detection antibodies to canine parvovirus[J].Chinese journal of preventive veterinary medicine,2009,(07):141.
[2]张 坤#,马丽娟#,张乃生,等.犬细小病毒环介导等温扩增快速检测方法的初步建立[J].中国预防兽医学报,2010,(04):263.
 ZHANG Kun#,MA Li-juan#,ZHANG Nai-sheng,et al.Rapid detction of canine parvovirus by loop-mediated isothermal amplification method[J].Chinese journal of preventive veterinary medicine,2010,(07):263.
[3]杨 慧,曲光刚,赵元楷,等.犬细小病毒LAMP检测方法的建立[J].中国预防兽医学报,2010,(07):550.
 YANG Hui,QU Guang-gang,ZHAO Yuan-kai,et al.Development of loop-mediated isothermal amplification method for detection of Canine parvovirus[J].Chinese journal of preventive veterinary medicine,2010,(07):550.
[4]刘大飞,王牟平,司 微,等.犬细小病毒黑龙江株(CPV-YN)的分离、鉴定及致弱的研究[J].中国预防兽医学报,2010,(11):875.[doi:10.3969/j.issn.1008-0589.2010.11.11]
 LIU Da-fei,WANG Mu-ping,SI Wei,et al.Attenuation of a canine parvovirus strain CPV-YN isolated from Heilongjiang province[J].Chinese journal of preventive veterinary medicine,2010,(07):875.[doi:10.3969/j.issn.1008-0589.2010.11.11]
[5]刘大飞,王牟平,曲连东,等.I型犬腺病毒黑龙江株(CAV-H)的分离、鉴定及致弱的研究[J].中国预防兽医学报,2010,(12):956.[doi:10.3969/j.issn.1008-0589.2010.12.11]
 LIU Da-fei,WANG Mu-ping,QU Lian-dong,et al.Identification and attenuation of H isolate of canine adenovirus typeⅠisolated in Heilongjiang area[J].Chinese journal of preventive veterinary medicine,2010,(07):956.[doi:10.3969/j.issn.1008-0589.2010.12.11]
[6]王亚君,华育平*,高光慧.重组犬瘟热病毒抗原表位T’TB和犬细小病毒vp2基因的真核表达[J].中国预防兽医学报,2008,(05):349.
 WANG Ya-jun,HUA Yu-ping*,GAO Guang-hui.Eukaryotic expression of recombined with T’TB antigen epitope of canine distemper virus and vp2 gene of canine parvovirus[J].Chinese journal of preventive veterinary medicine,2008,(07):349.
[7]戈 锐,彭广能*,夏咸柱*,等.犬细小病毒四川株的分离与鉴定[J].中国预防兽医学报,2007,(11):836.
 GE Rui,PENG Guang-neng*,XIA Xian-zhu*,et al.Isolation and identification of canine parvovirus Sichuan strains[J].Chinese journal of preventive veterinary medicine,2007,(07):836.
[8]黄永亮,施赫赫,冯顺意,等.广东珠三角地区犬细小病毒分离与鉴定[J].中国预防兽医学报,2012,(10):778.[doi:doi: 10.3969/j.issn.1008-0589.2012.10.05]
 HUANG Yong-liang,SHI He-he,FENG Shun-yi,et al.Isolation and identification of canine parvovirus inPearl River Delta region of Guangdong[J].Chinese journal of preventive veterinary medicine,2012,(07):778.[doi:doi: 10.3969/j.issn.1008-0589.2012.10.05]
[9]刘大飞*,姜一曈*,戚 亭,等.同时检测犬瘟热病毒、犬细小病毒、I型和II型犬腺病毒多重PCR方法的建立[J].中国预防兽医学报,2012,(11):911.[doi:doi: 10.3969/j.issn.1008-0589.2012.11.15]
 LIU Da-fei*,JIANG Yi-tong*,QI Ting,et al.Establishment of the multiplex PCR to the detection of CDV, CPV, CAV-I and CAV-II[J].Chinese journal of preventive veterinary medicine,2012,(07):911.[doi:doi: 10.3969/j.issn.1008-0589.2012.11.15]
[10]刘大飞#,姜一曈#,杨天阔,等.同时检测犬瘟热病毒和犬细小病毒双重PCR方法的建立[J].中国预防兽医学报,2012,(03):211.[doi:10.3969/j.issn.1008-0589.2012.03.11]
 LIU Da-fei#,JIANG Yi-tong#,YANG Tian-kuo,et al.Establishment of the duplex PCR for the detection of canine distemper virus and canine parvovirus[J].Chinese journal of preventive veterinary medicine,2012,(07):211.[doi:10.3969/j.issn.1008-0589.2012.03.11]

更新日期/Last Update: 2018-08-15