[1]侯文凤,林 辉,王凤求,等.伪狂犬病病毒GD1406变异株的抗原性分析[J].中国预防兽医学报,2018,(07):621-625.[doi:0.3969/j.issn.1008-0589.201711010]
 HOU Wen-feng,LIN Hui,WANG Feng-qiu,et al.Antigenic analysis of pseudorabies virus variant strain GD1406[J].Chinese journal of preventive veterinary medicine,2018,(07):621-625.[doi:0.3969/j.issn.1008-0589.201711010]
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伪狂犬病病毒GD1406变异株的抗原性分析()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年07
页码:
621-625
栏目:
免疫学
出版日期:
2018-08-15

文章信息/Info

Title:
Antigenic analysis of pseudorabies virus variant strain GD1406
文章编号:
1008-0589(2018)7-0621-05
作者:
 

侯文凤1林 辉1王凤求2赵玉林2伍建敏2王 衡1李中圣2*远立国1*

 (1. 华南农业大学 兽医学院,广东 广州 510642;2. 广东海大畜牧兽医研究院,广东 广州 510603)
Author(s):
 

HOU Wen-feng1 LIN Hui1 WANG Feng-qiu2 ZHAO Yu-lin2 WU Jian-min2 WANG Heng1LI Zhong-sheng2* YUAN Li-guo1*

 (1. College of Veterinary, South China Agricultural University, Guangzhou 510642, China;
2. Guangdong Institute of Animal Husbandry and Fishery, Guangzhou 510603, China)
关键词:
伪狂犬病病毒变异株ELISA中和试验
Keywords:
 pseudorabies virus variant  ELISA  neutralization test
分类号:
S852.65
DOI:
0.3969/j.issn.1008-0589.201711010
文献标志码:
A
摘要:
为了解近年来广东省伪狂犬病病毒(PRV)变异情况,本研究从猪场收集Bartha-K61活疫苗免疫后的猪血清559份,经ELISA筛选出326份gB抗体阳性且gE抗体阴性的猪血清,进行中和试验,分析血清中的疫苗抗体对Bartha-K61株和临床分离到的PRV GD1406野毒株的中和能力。进一步应用小鼠进行交叉免疫保护性试验。结果显示,187份gB阳性且gE阴性的猪血清样品对Bartha-K61株和PRV GD1406野毒株的平均中和抗体滴度分别为1∶57和1∶13,并且免疫Bartha-K61株使小鼠免受PRV GD1406野毒株致死性攻击的保护率仅为20 %,而免疫PRV GD1406野毒株使小鼠免受Bartha-K61株致死性攻击的保护率为100 %,免疫灭活PRV GD1406野毒株使小鼠免受PRV GD1406野毒株致死性攻击的保护率为40 %。根据试验结果推测,PRV GD1406野毒株与Batha-K61株之间存在抗原差异性,现用疫苗不能有效抵抗PRV变异株攻击。本研究结果将为PRV的防控提供实验依据,为研制PRV新疫苗提供新的思路。
  
Abstract:
To investigate the variation of pseudorabies virus (PRV) in Guangdong from 2011, a total of 559 vaccinated serum smples were collected from several pig farms to screen out the 326 gB-positive and gE-negative sera by ELISA. Neutralizing titers against Bartha-K61 and the PRV GD1406 strain were determined using cross-immunoprotection test in mice. The neutralization test results showed that average neutralizing titers of these 187 samples against Bartha-K61 and PRV GD1406 were 1∶57 and 1∶13, respectively. Bartha-K61 immunization was less effective against PRV GD1406 challenge, the survival rate of mice was 1/5. In contrast, PRV GD1406 immunization was effective against Bartha-K61 challenge, and provided completely protection for the mice. Inactivated PRV GD1406 immunization was less effective against PRV GD1406 challenge, and the survival rate of mice was 2/5. In conclusion, it is speculat that the PRV GD1406 has antigenic alterations compared with Bartha-K61 strain and conventional vaccines are less effective against new emergent PRVs challenge. The present study provides basis for the prevention and control of PRV and new ideas for the vaccine development.

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(本文编辑:李 爽)

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更新日期/Last Update: 2018-08-15