[1]朝洛蒙,王金良,布日额*,等.基于奶牛乳腺炎无乳链球菌PI-2a菌毛岛屿rAP1-BP-AP2重组蛋白的间接ELISA 方法的建立[J].中国预防兽医学报,2018,(07):611-615.[doi:0.3969/j.issn.1008-0589.201711003]
 CHAO Luo-meng,WANg Jin-liang,BU Ri-e*,et al.Establishment of an indirect ELISA for detecting the antibody against dairy mastitis Streptococcus agalactiae with its rAP1-BP-AP2 proteins as coating antigen[J].Chinese journal of preventive veterinary medicine,2018,(07):611-615.[doi:0.3969/j.issn.1008-0589.201711003]
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基于奶牛乳腺炎无乳链球菌PI-2a菌毛岛屿rAP1-BP-AP2重组蛋白的间接ELISA 方法的建立()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年07
页码:
611-615
栏目:
出版日期:
2018-08-15

文章信息/Info

Title:
Establishment of an indirect ELISA for detecting the antibody against dairy mastitis Streptococcus agalactiae with its rAP1-BP-AP2 proteins as coating antigen
文章编号:
1008-0589(2018)7-0611-05
作者:
朝洛蒙1王金良2布日额345*吴金花345孙立杰345锡林高娃345陈金龙6
(1. 内蒙古民族大学 动物科技学院,内蒙古 通辽 028043;2. 山东省滨州畜牧兽医研究院,山东 滨州 256600;3. 内蒙古民族大学 生命科学学院,内蒙古 通辽 028043;4. 内蒙古自治区乳源性致病菌防控工程技术研究中心,内蒙古 通辽 028043;
5. 内蒙古民族大学 乳源性致病菌研究所,内蒙古 通辽 028043;6. 山东绿都生物科技有限公司,山东 滨州 256600)
Author(s):
CHAO Luo-meng1 WANg Jin-liang2 BU Ri-e345* WU Jin-hua345 SUN Li-jie345XI Lin gao-wa345 CHEN Jin-long6
(1. College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028043, China; 2. Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, China; 3. College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028043, China; 4. Inner Mongolia Autonomous Region Engineering Technology Research Center of Prevention and Control the Pathogenic Bacteria in Milk, Tongliao 028043, China; 5. Research Institute for Pathogenic in Milk of Inner Mongolia University for Nationalities, Tongliao 028043, China; 6. Shandong Lvdu Bio-sciences and Technology Co., LTD, Binzhou 256600, China)
关键词:
牛乳腺炎无乳链球菌菌毛岛屿PI-2a重组蛋白AP1-BP-AP2ELISA
分类号:
S852.61
DOI:
0.3969/j.issn.1008-0589.201711003
文献标志码:
A
摘要:
为建立一种快速准确高效的检测奶牛乳腺炎无乳链球菌PI-2a菌毛岛屿抗原相应抗体的检测方法,本研究以牛乳腺炎无乳链球菌Ia型PI-2a菌毛岛屿辅助蛋白AP1、AP2及骨架蛋白BP 3基因串联表达的重组蛋白为ELISA包被抗原,通过方阵滴定法优化反应条件,建立了奶牛乳腺炎无乳链球菌抗体的间接ELISA检测方法。优化后抗原的最佳包被量为5.0 μg/孔,血清样品最佳稀释倍数为1∶40,酶标二抗最佳稀释倍数为1∶5 000。对S.agalactiae、S.pyogens、E.coli、S.aureus、S.epidermidis阳性血清进行检测结果显示,后4种阳性对照血清未出现阳性反应,表明该方法具有良好的特异性;对已知牛无乳链球菌阳性血清倍比稀释后进行检测时,当稀释倍数达到1:12 800时仍出现阳性结果,表明该方法具有较高的敏感度;重复性试验显示批内变异系数为2.41 %~8.89 %,批间试验变异系数为7.53 %~10.46 %;用该方法对426份临床样品进行奶牛无乳链球菌抗体检测,结果显示,其样品阳性检出率为45.07 %。本研究首次基于乳腺炎无乳链球菌PI-2a菌毛岛屿三联重组蛋白建立的间接ELISA 方法为奶牛无乳链球菌的快速检测提供了一种准确、可靠的检测方法。??

参考文献/References:

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更新日期/Last Update: 2018-08-15