[1]马旭升,何延华,盛金良,等.牛病毒性腹泻病毒BVDV-LC株的分离与鉴定[J].中国预防兽医学报,2018,(06):546-549.[doi:0.3969/j.issn.1008-0589.201707031]
 MA Xu-sheng,HE Yan-hua,SHENG Jin-liang,et al.Isolation and identification of a bovine viral diarrhea virus strain LC[J].Chinese journal of preventive veterinary medicine,2018,(06):546-549.[doi:0.3969/j.issn.1008-0589.201707031]
点击复制

牛病毒性腹泻病毒BVDV-LC株的分离与鉴定()
分享到:

《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年06
页码:
546-549
栏目:
研究简报
出版日期:
2018-06-20

文章信息/Info

Title:
Isolation and identification of a bovine viral diarrhea virus strain LC
文章编号:
1008-0589(2018)6-0546-04
作者:
 

马旭升1何延华23盛金良2黄 新3丁金花2豆晓霞2刘贤侠2陈创夫12*

 (1. 石河子大学 生命科学学院,新疆 石河子 832000;2. 石河子大学 动物科技学院,新疆 石河子 832000;
3. 省部共建绵羊遗传改良与健康养殖国家重点实验室/新疆农垦科学院,新疆 石河子832000)
Author(s):
 

MA Xu-sheng1 HE Yan-hua23 SHENG Jin-liang2 HUANG Xin3 DING Jin-hua2 DOU Xiao-xia2LIU Xian-xia2 CHEN Chuang-fu12*

 (1. College of Life Science, Shihezi University, Shihezi 832000, China; 2. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China; 3. State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang
Academy of Agricultural and Reclamation Science, Shihezi 832000, China)
关键词:
牛病毒性腹泻病毒分离鉴定进化分析
Keywords:
  bovine viral diarrhea virus  isolation and identification  phylogenetic analysis
分类号:
S852.65
DOI:
0.3969/j.issn.1008-0589.201707031
文献标志码:
A
摘要:
为分离鉴定新疆地区牛病毒性腹泻病毒(BVDV)流行株,本研究通过RT-PCR检测、细胞分离培养、直接免疫荧光抗体检测、核苷酸序列测定及生物信息学分析对新疆某奶牛场采集的腹泻牛粪便样品进行了病毒分离和鉴定。结果显示,分离得到一株新的牛源BVDV,命名为BVDV-LC株。该病毒株在MDBK细胞培养时未能引起细胞病变;5’-UTR与Npro PCR扩增为阳性,扩增片段与预期大小一致;直接免疫荧光检测荧光信号为阳性;病毒滴度为105.6 TCID50/0.1 mL;遗传进化分析显示,该分离株与猪源BVDV-SD0803株有较近的亲缘关系,同属于BVDV-1q基因亚型。本研究表明BVDV-LC株的分离鉴定对进一步开展疫苗研发、牛病毒性腹泻致病机理等方面的研究具有重要意义。
  
Abstract:
In the present study, a virus was isolated from faeces samples of cattle with bovine viral diarrhea like syndrome in a dairy farm in Xinjiang area, and identificated by RT-PCR, cell culture, direct immunofluorescent assay, nucleotide sequencing and bioinformatics analysis. The results showed that the virus isolate was bovine viral diarrhea virus (BVDV) and designated BVDV-LC. Although the virus was unable to produce cytopathic effect (CPE) in MDBK cells, it was capable to replicate efficiently in MDBK with a virus titer of 105.6 TCID50/0.1 mL detected by PCR amplification of the 5’-UTR and Npro, or direct immunofluorescent assay. Phylogenetic analysis indicated that the isolate was close to BVDV-SD0803 of swine origin, both of which belonged to BVDV-1q gene subtype. The isolation and identification of BVDV-LC strain was of great value to the vaccine development and the study of bovine viral diarrhea.

参考文献/References:

[1]Mayo M A. A summary of taxonomic changes recently approved by ICTV [J]. Arch Virol, 2002, 147(8): 1655.
[2]Rasmussen T B, Uttenthal A, Reimann I, et al. Virulence, immunogenicity and vaccine properties of a novel chimeric pestivirus [J]. J Gen Virol, 2007, 88(Pt 2): 481-486.
[3]Ridpath J F, Bolin S R, Dubovi E J. Segregation of bovine diarrhea virus into genotypes [J]. Virology, 1994, 205(1): 66.
[4]Deng Ming-liang, Ji Su-kun, Fei Wen-tao, et al. Prevalence study and genetic typing of bovine viral diarrhea virus (BVDV) in four bovine species in China [J]. PLoS One, 2015, 10(4): e0121718.
[5]Giammarioli M, Ceglie L, Rossi E, et al. Increased genetic diversity of BVDV-1: recent findings and implications thereof [J]. Virus Genes, 2015, 50(1): 147-151.
[6]Xue Fei, Zhu Yuan-mao, Li Jiao, et al. Genotyping of bovine viral diarrhea viruses from cattle in China between 2005 and 2008 [J]. Vet Microbiol, 2010, 143(2-4): 379.
[7]Bauermann F V, Ridpath J F, Weiblen R, et al. HoBi-like viruses: an emerging group of pestiviruses [J]. J Vet Diagn Invest, 2013, 25(1): 6.
[8]Altamiranda E A G, Kaiser G G, Weber N, et al. Clinical and reproductive consequences of using BVDV-contaminated semen in artificial insemination in a beef herd in Argentina [J]. Anim Reprod Sci, 2012, 133(3-4): 146-152.
(本文编辑:李    爽)

相似文献/References:

[1]李春芬,李 郁*,魏建忠,等.安徽省部分地区鸭疫里氏杆菌的分离鉴定及生物学特性研究[J].中国预防兽医学报,2009,(03):197.
 LI Chun-fen,LI Yu*,WEI Jian-zhong,et al.Isolation and biological characterization of Riemerella anatipestifer in Anhui province [J].Chinese journal of preventive veterinary medicine,2009,(06):197.
[2]师东方,葛俊伟,王明翠,等.一株混合O 血清型猪水肿病大肠杆菌的分离与鉴定[J].中国预防兽医学报,2009,(05):397.
 SHI Dong-fang,GE Jun-wei,WANG Ming-cui,et al.Isolation and Identification of a Escherichia coli strain of Mixed O serotypes in piglet[J].Chinese journal of preventive veterinary medicine,2009,(06):397.
[3]许传田,董国英,杨焕良,等.人源H3N2亚型猪流感病毒A/Swine/Guangdong/1/2005 的分离鉴定及全基因遗传演化分析[J].中国预防兽医学报,2009,(04):322.
 XU Chuan-tian,DONG Guo-ying,YANG Huan-liang,et al.Isolation and identification of human influenza virus A/swine/Guangdong/1/05 (H3N2)[J].Chinese journal of preventive veterinary medicine,2009,(06):322.
[4]许传田,樊树芳,杨焕良,等.H1N2亚型猪流感病毒A/Swine/Guangdong/1/06分离鉴定和对猪致病性研究 [J].中国预防兽医学报,2009,(01):46.
 XU Chuan-tian*,FAN Shu-fang*,YANG Huan-liang,et al.Isolation and identification of swine influenza virus A/Swine/Guangdong/1/06(H1N2) [J].Chinese journal of preventive veterinary medicine,2009,(06):46.
[5]范书才*,李 虹,袁率珍,等.新型鸭肝炎病毒的分离鉴定 [J].中国预防兽医学报,2009,(10):770.
 FAN Shu-cai*,LI Hong,YUAN Shuai-zhen,et al.Isolation and characterization of a new serotype of duck hepatitis virus [J].Chinese journal of preventive veterinary medicine,2009,(06):770.
[6]尹 录,杜艳芬,赫明雷,等.哈尔滨市鲜肉中单核细胞增生性李斯特菌的分离鉴定及耐药性分析[J].中国预防兽医学报,2009,(12):929.
 YIN Lu,DU Yan-fen,HE Ming-lei,et al.Isolation and identification of antibiotic resistance of Listeria monocytogenes isolated from meat in Harbin [J].Chinese journal of preventive veterinary medicine,2009,(06):929.
[7]吴晓平,钱 琨,秦爱建*,等.商品蛋鸡J亚群禽白血病病毒分离鉴定及序列分析[J].中国预防兽医学报,2010,(05):351.
 WU Xiao-ping,QIAN Kun,QING Ai-jian*,et al.Isolation and sequences analysis of subgroup J avian leukosis viruses in commercial layer chickens[J].Chinese journal of preventive veterinary medicine,2010,(06):351.
[8]马文戈,于 力*.牛源荚膜血清A型多杀性巴氏杆菌的分离鉴定[J].中国预防兽医学报,2008,(10):747.
 MA Wen-ge,YU Li*.Isolation and identification of bovine capsular serotype A Pasteurella mutocida[J].Chinese journal of preventive veterinary medicine,2008,(06):747.
[9]常继涛,崔久辉,李建军,等.我国部分地区牛轮状病毒的病原学调查及一株G10P[11]型牛轮状病毒的分离鉴定[J].中国预防兽医学报,2008,(10):755.
 CHANG Ji-tao,CUI Jiu-hui,LI Jian-jun,et al.Etiologic investigation of bovine rotavirus in the some regions of China and isolation of a G10P[11] rotavirus[J].Chinese journal of preventive veterinary medicine,2008,(06):755.
[10]高 晗,闫喜军*,柴秀丽,等.犬瘟热病毒强毒株HBF-1株的培育及致病性研究[J].中国预防兽医学报,2010,(12):929.[doi:10.3969/j.issn.1008-0589.2010.12.04]
 GAO Han,YAN Xi-jun*,CHAI Xiu-li,et al.Isolation and identification of a canine distemper virus HBF-1 from fox[J].Chinese journal of preventive veterinary medicine,2010,(06):929.[doi:10.3969/j.issn.1008-0589.2010.12.04]
[11]张国华,吴丹丹,周玉龙*,等.牛病毒性腹泻病毒黑龙江分离株的分离鉴定[J].中国预防兽医学报,2015,(10):805.[doi:10.3969/j.issn.1008-0589.2015.10.16]
 ZHANG Guo-hua,WU Dan-dan,ZHOU Yu-long*,et al.Isolation and identification of bovine viral diarrhea virusfrom Heilongjiang province[J].Chinese journal of preventive veterinary medicine,2015,(06):805.[doi:10.3969/j.issn.1008-0589.2015.10.16]

更新日期/Last Update: 2018-07-12