[1]张志强,杨 楠,李永慧,等.迟缓爱德华菌外膜蛋白OmpA原核表达及其免疫原性研究[J].中国预防兽医学报,2018,(06):534-537.[doi:0.3969/j.issn.1008-0589.201705034]
 ZHANG Zhi-qiang,YANG Nan,LI Yong-hui,et al.Prokaryotic expression and immunological protection analysis for OmpA protein of Edwardsiella.tarda[J].Chinese journal of preventive veterinary medicine,2018,(06):534-537.[doi:0.3969/j.issn.1008-0589.201705034]
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迟缓爱德华菌外膜蛋白OmpA原核表达及其免疫原性研究()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年06
页码:
534-537
栏目:
免疫学
出版日期:
2018-06-20

文章信息/Info

Title:
Prokaryotic expression and immunological protection analysis for OmpA protein of Edwardsiella.tarda
文章编号:
1008-0589(2018)6-0534-04
作者:
 

张志强1杨 楠1李永慧2王洪彬1冯东青1吴同垒1史秋梅1*朱国强3*

 (1. 河北科技师范学院 河北省预防兽医学重点实验室,河北 秦皇岛 066004;2. 秦皇岛第二医院,河北 秦皇岛 066604;
3. 扬州大学, 江苏 扬州 225009)
Author(s):
 

ZHANG Zhi-qiang1 YANG Nan1 LI Yong-hui2 WANG Hong-bin1 FENG Dong-qing1 WU Tong-lei1SHI Qiu-mei1* ZHU Guo-qiang3*

 (1. Hebei Key Laboratory of Preventive Veterinary Medicine, Hebei Normal University of Science &Technology, Qinhuangdao 066004, China;
2. The Second Hospital of Qinhuangdao, Qinhuangdao 066604, China;
3. Yangzhou university, Yangzhou 225009, China)
关键词:
迟缓爱德华菌外膜蛋白OmpA蛋白表达免疫保护力
Keywords:
 Edwardsiella tarda  outer membrane protein  OmpA  protein expression  immunological protection
分类号:
R318
DOI:
0.3969/j.issn.1008-0589.201705034
文献标志码:
A
摘要:
为研究迟缓爱德华菌外膜蛋白OmpA免疫保护性,本研究利用PCR方法扩增迟缓爱德华菌ompA基因,构建重组载体pET-32a-ompA,将其转化大肠杆菌BL21后诱导表达,表达产物经SDS-PAGE 和western blot 分析显示,重组蛋白大小约58 ku;将纯化的重组蛋白免疫小鼠后,以迟缓爱德华菌强毒株ET-13攻毒,结果显示该重组蛋白对免疫组小鼠具有保护力,保护率为 55 %。本研究克隆了迟缓爱德华菌ompA基因并表达了相应重组蛋白,免疫小鼠后能够提供一定保护,为重组 OmpA蛋白亚单位疫苗的研制奠定基础。
 
Abstract:
To study the immunoprotection of OmpA against Edwardsiella tarda in mice, the encoding gene, ompA, was amplified by PCR from E.tarda ET-13 and cloned into the pET-32a vector. The recombinant plasmid was transformed into E.coli BL21 (DE3) cells, in which a recombinant OmpA  protein (rOmpA) was expressed by inducing with IPTG. SDS-PAGE and western blot analysis showed that the expressed rOmpA was about 60ku which was recognized by the positive serum against E.tarda. The mice were immunized intramuscularly with purified rOmpA, and challenged with the E.tarda ET-13. Result showed that the rOmpA provided a 55% protection for the immunized mice. The data demonstrated that the OmpA could be used as the antigen for developing the subunit vaccine.

参考文献/References:

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                                            (本文编辑:李 娜)

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更新日期/Last Update: 2018-07-12