[1]王晓利,廖成水*,路 霞,等.鼠伤寒沙门菌重组鞭毛蛋白对巨噬细胞细胞因子与氧化/抗氧化水平的影响[J].中国预防兽医学报,2018,(06):528-533.[doi:0.3969/j.issn.1008-0589.201707017]
 WANG Xiao-li,LIAO Cheng-shui*,LU Xia,et al.Effects of the recombinant flagellin protein of Salmonella typhimurium on cytokine and oxidation/antioxidation in macrophage[J].Chinese journal of preventive veterinary medicine,2018,(06):528-533.[doi:0.3969/j.issn.1008-0589.201707017]
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鼠伤寒沙门菌重组鞭毛蛋白对巨噬细胞细胞因子与氧化/
抗氧化水平的影响
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年06
页码:
528-533
栏目:
免疫学
出版日期:
2018-06-20

文章信息/Info

Title:
Effects of the recombinant flagellin protein of Salmonella typhimurium on cytokine and oxidation/antioxidation in macrophage
文章编号:
1008-0589(2018)6-0528-06
作者:
 

王晓利1廖成水24*路 霞2崔淹鸽3王安琪4王 臣2

 (1. 河南科技大学 医学院,河南 洛阳 471023;2. 河南科技大学 动物科技学院/洛阳市活载体生物材料与动物疫病防控重点实验室,河南 洛阳 471023;3. 洛阳市动物疫病预防控制中心,河南 洛阳 471002;4. 吉林大学 人兽共患病研究所/人兽共患病研究教育部重点实验室,吉林  长春 130062)
Author(s):
 

WANG Xiao-li1 LIAO Cheng-shui24* LU Xia2 CUI Yan-ge3 WANG An-qi4 WANG Chen2

 (1. Medical College, Henan University of Science and Technology, Luoyang 471023, China; 2. Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control/College of animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China; 3. Luoyang Animal Disease Prevention and Control Center, Luoyang 471002, China;
4. Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun 130062, China)
关键词:
鼠伤寒沙门菌鞭毛蛋白巨噬细胞细胞因子氧化/抗氧化
分类号:
S855.1
DOI:
0.3969/j.issn.1008-0589.201707017
文献标志码:
A
摘要:
为研究重组鼠伤寒沙门菌鞭毛蛋白对巨噬细胞细胞因子与氧化/抗氧化水平的影响,本研究将50 ng/mL、250 ng/mL和500 ng/mL重组鼠伤寒沙门菌鞭毛蛋白FliC分别与巨噬细胞J774.1共孵育,分别采用CCK-8法、LDH试剂盒、ELISA试剂盒和荧光显微镜检测巨噬细胞活力、巨噬细胞上清乳酸脱氢酶(LDH)、巨噬细胞分泌细胞因子和巨噬细胞胞内活性氧(ROS)水平;采用商品化试剂盒检测巨噬细胞产生的诱导型一氧化氮合酶(iNOS)、丙二醛(MDA)、一氧化氮(NO)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)和总抗氧化能力(T-AOC)等氧化/抗氧化水平。结果显示,50 ng/mL重组鼠伤寒沙门菌鞭毛蛋白对巨噬细胞活力无影响,而巨噬细胞活力在该蛋白浓度为250 ng/mL (12 h)和500 ng/mL (9 h)时明显下降。该重组蛋白对巨噬细胞上清LDH水平的影响随其浓度的增加呈上升趋势,同时该重组蛋白的浓度对巨噬细胞分泌IL-1β、IL-4、IL-6、IL-10、IL-12、TNF-α和TGF-β的影响也呈正相关趋势。荧光显微镜检测显示,该重组蛋白刺激巨噬细胞胞内ROS水平明显增强。氧化/抗氧化水平方面,该重组蛋白导致巨噬细胞iNOS、MDA和NO氧化水平升高,而GSH-PX、SOD和T-AOC抗氧化水平降低。综上所述,鼠伤寒沙门菌重组鞭毛蛋白能增强巨噬细胞分泌细胞因子,也可引起巨噬细胞氧化/抗氧化平衡失调,为进一步探究鼠伤寒沙门菌与宿主的互作提供参考。
  
Abstract:
To investigate the effects of the recombinant flagellin FliC (rFliC) of Salmonella typhimurium on cytokine and oxidation/antioxidation levels in macrophage, the macrophage J774.1 were co-cultured with 50ng/mL, 250ng/mL, and 500ng/mL of rFliC, respectively, then the activities of macrophages were analyzed by CCK-8 kit. The level of lactate dehydrogenase (LDH), cytokines, and reactive oxygen species (ROS) level in macrophages were detected by LDH cytotoxicity assay kit, ELISA kit, and

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                                             (本文编辑:李 娜)

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更新日期/Last Update: 2018-07-12