[1]冷依伊,王 印*,项明源,等.裂谷热病毒两种PCR检测方法的建立及比较分析[J].中国预防兽医学报,2018,(06):504-508.[doi:0.3969/j.issn.1008-0589.201709002]
 LENG Yi-yi,WANG Yin*,XIANG Ming-yuan,et al.The comparation of two PCR methods for detection of rift valley fever virus[J].Chinese journal of preventive veterinary medicine,2018,(06):504-508.[doi:0.3969/j.issn.1008-0589.201709002]
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裂谷热病毒两种PCR检测方法的建立及比较分析()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年06
页码:
504-508
栏目:
诊断和检测技术
出版日期:
2018-06-20

文章信息/Info

Title:
The comparation of two PCR methods for detection of rift valley fever virus
文章编号:
1008-0589(2018)6-0504-05
作者:
 

冷依伊12王 印12*项明源12张鹏飞12蒋子睿12江地科12杨泽晓1姚学萍1

 (1. 四川农业大学 动物医学院,四川 成都 611130;
2. 动物疫病与人类健康四川省重点实验室,四川 成都 611130)
Author(s):
 

LENG Yi-yi12 WANG Yin12* XIANG Ming-yuan12 ZHNAG Peng-fei12 JIANG Zi-rui12JIANG Di-ke12 YANG Ze-xiao1 YAO Xue-ping1

 (1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China)
关键词:
 关键词:裂谷热病毒探针PCR荧光PCR
Keywords:
  Rift Valley fever virus  TaqMan probe real-time PCR  fluorescence SYBR Green I based quantitative PCR
分类号:
S852.65
DOI:
0.3969/j.issn.1008-0589.201709002
文献标志码:
A
摘要:
裂谷热(RVF)具有较高的发病率与死亡率,极大危害着当地养殖业和生态系统的稳定。为建立一种能够快速有效检测裂谷热病毒(RVFV)的方法,本研究根据RVFV的保守序列设计了特异性引物与探针,分别建立了探针PCR和荧光PCR,并对这两种检测方法进行比较分析。结果显示,两种检测方法均不存在非特异扩增;最低检测浓度分别为1.03×10-2拷贝/μL和1.03×100拷贝/μL,前者比后者有更高的灵敏度;探针PCR和荧光PCR组内和组间变异系数均小于3 %。实验表明,探针PCR具有特异性强、灵敏度高、稳定性好、准确度高、检测速度快等优点。因此,探针PCR是一种灵敏度和可靠性均较高的RVFV检测方法。
  
Abstract:
Rift Valley fever (RVF) has high morbidity and mortality, which seriously endangers the stability of local livestock and ecosystem. In order to establish a rapid and effective method for detection of RVF virus (RVFV), the TaqMan real-time PCR, fluorescence SYBR Green I based quantitative PCR (FQ PCR) were established with the specific primers and probes designed according to the conserved sequence of RVFV, and compared for the RVFV detections. The results showed that TaqMan real-time PCR had the advantages of strong specificity, high sensitivity and stability with fast detection speed. The results of this study showed that although the TaqMan real-time PCR and FQ PCR were less than 3% for the coefficient of variation within and between groups, but the former had higher sensitivity than the latter, and the minimum detectable concentration of the two detection methods were 1.03×10-2 copies/μL and 1.03×100 copies/μL of RVFV for the TaqMan real-time PCR and FQ PCR, and there was no nonspecific amplification. Therefore, the TaqMan real-time PCR was a high priority method for RVFV detection.

参考文献/References:

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(本文编辑:李 

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更新日期/Last Update: 2018-07-12