[1]马洪梅,牛家强*,张海燕,等.一株不依赖NAD生长的副鸡禽杆菌的分离与鉴定[J].中国预防兽医学报,2018,(06):500-503.[doi:0.3969/j.issn.1008-0589.201711033]
 MA Hong-mei,NIU Jia-qiang*,ZHANG Hai-yan,et al.Isolation and identification of a NAD-independent Avibacterium paragallinarum[J].Chinese journal of preventive veterinary medicine,2018,(06):500-503.[doi:0.3969/j.issn.1008-0589.201711033]
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一株不依赖NAD生长的副鸡禽杆菌的分离与鉴定()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年06
页码:
500-503
栏目:
病原生物学
出版日期:
2018-06-20

文章信息/Info

Title:
Isolation and identification of a NAD-independent Avibacterium paragallinarum
文章编号:
1008-0589(2018)6-0500-04
作者:
 

马洪梅12牛家强1*张海燕2刘洋洋2胡思顺23*许青荣2李自力23刘 梅23毕丁仁23

 (1. 西藏农牧学院 动物科学学院/高原动物疫病研究自治区高校重点实验室,西藏 林芝 860000;2. 华中农业大学 动物医学院/
农业部兽用诊断制剂创制重点实验室,湖北 武汉 430070;3. 华中农业大学/湖北省预防兽医学重点实验室,湖北 武汉 430070)
Author(s):
 

MA Hong-mei12 NIU Jia-qiang1* ZHANG Hai-yan2 LIU Yang-yang2 HU Si-shun23* XU Qing-rong2 LI Zi-li23 LIU Mei23 BI Ding-ren23

 (1. Provincial Key Laboratory of Tibet Plateau Animal Epidemic Disease Research, Zizang Agricultural and Animal Husbandry College, Department of Veterinary Medicane Nyingzhi, Tibet 860000, China; 2. Key Laboratory of Development of Veterinary Diagnostic Products of Ministry  Medicine, Huazhong Agricultural University, College of Veterinary Medicine, Wuhan 430070, China; 3. Key Laboratory of Preventive Veterinary Medicine of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China)
关键词:
副鸡禽杆菌NAD质粒
Keywords:
  Avibacterium paragallinarum  NAD  plasmid
分类号:
S852.65
DOI:
0.3969/j.issn.1008-0589.201711033
文献标志码:
A
摘要:
为研究湖北省内鸡传染性鼻炎病原菌的特性,本研究从湖北恩施某地鸡场疑似传染性鼻炎的病鸡样品中分离获得一株疑似副鸡禽杆菌(APG)的病原菌。通过细菌的分离培养、形态观察、生化试验、革兰氏染色、动物回归试验和PCR等方法对其进行鉴定。结果显示,分离的病原菌具有APG的形态特征和生化特性;16S rRNA序列比对分析与已报道的APG相应序列同源性高于98 %;另外,利用特异PCR,分别检测到p250,pA14和pYMH5等3种质粒的特异基因片段;通过培养试验,该分离株能在无NAD的TSB (含鸡血清)中生长。这是国内首次发现无需NAD且同时存在3种质粒的APG。这一的发现警示国内的APG流行情况复杂,应引起畜牧兽医工作者的注意。
  
Abstract:
Avibacterium paragallinarum is a causative agent of avian infectious coryza in chicken. To understand the characteristics of A.paragallinarum, a A.paragallinarum  was isolated from chicken in Enshi, Hubei province, and identified by bacterial isolation and culture, morphological observation, biochemical tests, PCR and animal infection test. As results, the characteristics of the morphology, the biochemical tests and 16S rRNA sequence (more than 98% homology) of the isolate were identity to the A.paragallinarum reference strains. In addition, the isolate had the ability to grow in TSB medium containing chicken serum without NAD. Moreover,  the 3 plasmids of p250, pA14 and pYMH5 were detected in the isolate by PCR. This is the first report of NAD-independent and embodied the 3 plasmids of A.paragallinarum in China. Our results suggested that the A.paragallinarum infection was becoming more and more complex, which should be paid attention to by specialists.

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更新日期/Last Update: 2018-07-12