[1]李 晴?,张 泽?,曹甸磊,等.养殖废水产ESBLs大肠杆菌多重耐药性及耐药基因检测[J].中国预防兽医学报,2018,(06):495-499.[doi:0.3969/j.issn.1008-0589.201709022]
 LI Qing,ZHANG Ze,CAO Dian-lei,et al.Detection of multiple drug resistance and the resistance genes of ESBL-producing Escherichia coli from livestock wastewater[J].Chinese journal of preventive veterinary medicine,2018,(06):495-499.[doi:0.3969/j.issn.1008-0589.201709022]
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养殖废水产ESBLs大肠杆菌多重耐药性及耐药基因检测()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年06
页码:
495-499
栏目:
病原生物学
出版日期:
2018-06-20

文章信息/Info

Title:
Detection of multiple drug resistance and the resistance genes of ESBL-producing Escherichia coli from livestock wastewater
文章编号:
1008-0589(2018)6-0495-05
作者:
 

李 晴1?张 泽1? 曹甸磊2常维山1王 慧1王雪鹏1*

 (1. 山东省动物生物工程与疾病防治重点实验室 山东农业大学动物科技学院,山东 泰安 271018;
2. 临沂市兰山区畜牧兽医局,山东 临沂 276000)
Author(s):
 

LI Qing1? ZHANG Ze11? CAO Dian-lei2 CHANG Wei-shan1 WANG Hui1 WANG Xue-peng1*

 (1. Key Laboratory of Animal Biological Engineering and Disease Prevention and Control in Shandong,
College of Animal Science, Shandong Agricultural University, Tai’an 271018, China
2. Linyi Lanshan District Animal Husbandry and Veterinary Bureau, Linyi 276000, China)
关键词:
超广谱β-内酰胺酶大肠杆菌耐药表型耐药基因多重耐药
Keywords:
 extended-spectrum beta- lactamases  Escherichia coli  drug resistant phenotype  drug resistance gene multidrug resistance
分类号:
S852.61
DOI:
0.3969/j.issn.1008-0589.201709022
文献标志码:
A
摘要:
为研究养殖废水中产超广谱β-内酰胺酶(ESBLs)大肠杆菌的分布及其多重耐药性与耐药基因携带情况,本研究对泰安市污水处理厂污水中分离的50株产ESBLs大肠杆菌阳性菌株采用纸片扩散法测定其对19种常见药物的耐药表型。针对产ESBLs菌株的耐药基因设计6对特异性引物,对ESBLs阳性菌株采用PCR方法检测其耐药基因。结果显示,50株产ESBLs大肠杆菌对β-内酰胺类抗生素表现出较强的耐药性,其中对氨苄西林、头孢噻吩、头孢噻肟呈100 %的耐药性;对非β-内酰胺类抗生素同样呈一定耐药性;并且呈多重耐药性。PCR扩增结果显示,分离菌株均含有blaTEM、blaCTX-M基因,有5株含有qrB基因,32株含有qrS基因,有2株不含blaSHV基因,50株菌株均不含qrA基因。本研究表明:养殖场排放的污水已被产ESBL大肠杆菌严重污染,污水是这些耐药基因重要的存储库。且养殖污水中耐药菌通过污水排放最终进入到水环境中,其耐药基因可能在水环境的细菌之间进一步扩散,导致产ESBLs大肠杆菌表现出多重耐药性,同时这些产ESBL大肠杆菌会反过来再次感染动物,造成耐药菌在动物间的传播,甚至造成严重的感染。本研究对水是这些耐药基因重要的存储库这一结论提供了实验佐证。
  
Abstract:
 To examine the multiple drug resistance and the resistance genes in extended-spectrum beta-lactamases (ESBL) -producing Escherichia coli (ESBL-producing E.coli) from livestock wastewater, a total of 50 ESBL-producing E.coli were isolated from Tai’an city sewage treatment plant and subjected to detection of the 19 kinds of common drug resistance phenotype by disk diffusion method and the resistance genes with 6 pairs of specific primers by PCR. The results showed that the 50 isolates of the ESBL-producing E.coli displayed strong multiple resistance, which were not only 100% resistance to the β-lactam antibiotics of ampicillin, cephalothin and cefotaxime, but also resistannce to some non -β-lactam antibiotics. In addition, all the isolates contained blaTEM and blaCTX-M, genes and only two isolates without blaSHV. The quinolone resistance gene detection indicated that 5 isolates contained qrB and 32 isolates contained qrS, but no qrA was detected in all the 50 isolates. This study showed that the drug resistant bacteria in livestock wastewater would eventually enter into the water environment through discharge, and the drug-resistant genes might further spread among the bacteria in the aquatic environment, resulting in multiple resistance to Enterobacter ESBLs producing. These results provide the evidences for the effluent from farms is seriously polluted by Enterobacter ESBL and the water is an important repository for these resistant genes.

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(本文编辑:李 娜)

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更新日期/Last Update: 2018-07-12