[1]袁 伟,张德锋,可小丽,等.中国罗非鱼主养区无乳链球菌流行菌株的菌毛岛屿及其血清型分型[J].中国预防兽医学报,2018,(06):490-494.[doi:0.3969/j.issn.1008-0589.201610030]
 YUAN Wei,ZHANG De-feng,KE Xiao-li,et al.The pilus islands and molecular serotype ofStreptococcus agalactiae isolated from tilapia in the major tilapia-culturing areas in China[J].Chinese journal of preventive veterinary medicine,2018,(06):490-494.[doi:0.3969/j.issn.1008-0589.201610030]
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中国罗非鱼主养区无乳链球菌流行菌株的菌毛岛屿及其血清型分型()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年06
页码:
490-494
栏目:
出版日期:
2018-06-20

文章信息/Info

Title:
The pilus islands and molecular serotype of
Streptococcus agalactiae isolated from tilapia in the major tilapia-culturing areas in China
文章编号:
1008-0589(2018)6-0490-05
作者:
 

袁 伟12张德锋1可小丽1刘志刚1高风英1卢迈新1*

 (1. 中国水产科学研究院 珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室,广东 广州 510380;
2. 上海海洋大学 水产与生命学院,上海 201306)
Author(s):
 

YUAN Wei12 ZHANG De-feng1 KE Xiao-li1 LIU Zhi-gang1 GAO Feng-ying1 LU Mai-xin1*

 (1. Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture,
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;
2. College of Fisheries &Life, Shanghai Ocean University, Shanghai 201306, China)
关键词:
罗非鱼无乳链球菌菌毛岛屿分子血清型
Keywords:
tilapiastreptococcus agalactiae pilus island serotype
分类号:
S917.1
DOI:
0.3969/j.issn.1008-0589.201610030
文献标志码:
A
摘要:
为了解近年来中国罗非鱼主养区无乳链球菌流行特征,本研究通过PCR扩增和测序等方法对2007年~2016年分离纯化得到的263株罗非鱼无乳链球菌进行菌毛岛屿和血清型分型分析。菌毛岛屿分型结果显示:263株罗非鱼无乳链球菌可分为两种菌毛岛屿类型:PI-2b型(40株)和PI-1+PI-2b型(218株),其中PI-1+PI-2b型无乳链球菌是主要的流行菌株,占总数的82.9 %。其余5株无乳链球菌的菌毛岛屿基因缺失,不含菌毛岛屿。分子血清型分析结果显示,所有的无乳链球菌分为两种血清型:Ia型258株(98.1 %)和Ib型5株(1.9 %)。所有Ib型无乳链球菌的菌毛岛屿均有缺失,仅含有2b-AP1基因。进一步分析表明,Ia-(PI-2b)型无乳链球菌是2011年之前主要的流行株型,而Ia-(PI-1+PI-2b)型无乳链球菌为近6年来广泛流行的株型。Ia型是我国罗非鱼无乳链球菌最流行的分子血清型,同时PI-2b是最重要的菌毛岛屿(98.1 %)。本研究为罗非鱼无乳链球菌的流行病学和疾病防控提供调查依据。
 
Abstract:
The aim of this study is to analyze the molecular characteristics of Streptococcus agalactiae isolated from tilapia in the major tilapia-culturing areas in China. In the present study, a total of 263 S.agalactiae isolates were subjected to examination of molecular serotype and pilus islands by PCR and sequencing. The results showed that the 218 S.agalactiae possessed the PI-1+PI-2b pilus islands and 40 S.agalactiae had the PI-2b pilus islands, and the remaining 5 S.agalactiae had no pilus islands. Molecular serotype detection indicated that the 258 S.agalactiae (98.1%) were serotype Ia, and the remaining 5 S.agalactia (1.9%) were Ib. In addition, all Ib S.agalactiae were negative for pilus island detection, which only carried 2b-AP1 gene. Importantly, Ia-(PI-1) S.agalactiae was the predominant before 2011, but since then the Ia-(PI-1+PI-2b) S.agalactiae was the predominant instead. In conclusion, serotype Ia S.agalactiae was the most prevalence serotype in China, and the most important pilus islands were the PI-2b (98.1%). This study provided a basis framework for epidemiological analysis and prevention of the  streptococcosis in tilapia.

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                                               (本文编辑:彭永刚)

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更新日期/Last Update: 2018-07-12