[1]罗怡琳,蒙正群,周丽君,等.一株羊源海藻糖比伯斯坦菌的分离鉴定及生物学分析[J].中国预防兽医学报,2018,(06):486-489.[doi:0.3969/j.issn.1008-0589.201709024]
 LUO Yi-lin,MENG Zheng-qun,ZHOU Li-jun,et al. Identification and biological analysis of Bibersteinia trehalosi isolated from sheep[J].Chinese journal of preventive veterinary medicine,2018,(06):486-489.[doi:0.3969/j.issn.1008-0589.201709024]
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一株羊源海藻糖比伯斯坦菌的分离鉴定及生物学分析()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年06
页码:
486-489
栏目:
病原生物学
出版日期:
2018-06-20

文章信息/Info

Title:
 

Identification and biological analysis of Bibersteinia trehalosi isolated from sheep

文章编号:
1008-0589(2018)6-0486-04
作者:
 

罗怡琳1蒙正群1周丽君1冷依伊1尹清清1王 印12姚学萍1杨泽晓1*

 (1. 四川农业大学 动物医学院,四川 温江 611130;2. 动物疫病与人类健康四川省重点实验室,四川 温江 611130)
Author(s):
LUO Yi-lin1 MENG Zheng-qun1 ZHOU Li-jun1 LENG Yi-yi1 YIN Qing-qing1 WANG Yin12YAO Xue-ping1 YANG Ze-xiao1*
  (1. College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China;
2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Wenjiang 611130, China)
关键词:
海藻糖比伯斯坦菌分离鉴定药敏试验耐药基因
Keywords:
Bibersteinia trehalosi  isolation  identification  drug susceptibility test  resistance genes
分类号:
S852.61
DOI:
0.3969/j.issn.1008-0589.201709024
文献标志码:
A
摘要:
海藻糖比伯斯坦菌(Bibersteinia trehalosi)为人兽共患菌,但在我国的研究报道转少。本研究从病死羔羊肺脏组织中分离到一株革兰阴性短杆菌,对其进行了细菌分离、生化试验、16S rDNA和ropB基因的PCR扩增、小鼠致病性试验、药敏试验及四环素抗生素类与产超广谱β-内酰胺酶(ESBLs)等6种耐药基因的PCR检测。结果显示,该菌16S rDNA扩增序列与GenBank中B.trehalosi同源性达99 %;其特异性rpoB基因 PCR扩增条带与预期一致;对小鼠致病性强;对头孢哌酮、头孢呋辛、复方新诺明、氯霉素等多种抗生素均敏感,对青霉素、丁胺卡那、头孢拉啶耐药,仅扩增出约1 900 bp的BCT耐药基因片段,与公布的BCT基因序列同源性达99 %,耐药表型与耐药基因检测结果一致,证明该分离菌株为B.trehalosi。本研究为该菌的有效防控与致病机理的研究奠定了基础,也为养殖临床用药提供参考。
 
Abstract:
Bibersteinia trehalosi glycosis is a zoonosis bacterial species, but it was barely studied in China. In this study, a small gram-negative bacillus bacteria was isolated from lung tissue of a died lamb and identified by morphological and cultured characteristics, biochemical tests, 16S rDNA gene and specific ropB gene PCR amplification and sequencing, pathogenicity tests, and drug sensitivity tests. The drug-resistance genes were detected and analyzed by PCR methods using 6 pairs of primers for detecting drug-resistance genes that produce tetracycline and extended-spectrum β-lactamase (ESBLs) etc. The results showed that 16S rDNA gene sequence of the isolate shared 99% homology with B.trehalosi gene sequences available in GenBank, and the specific rpoB gene PCR amplification results was positive. The isolate was pathogenic to mice, and sensitive to cefoperazone, cefuroxime, co-trimoxazole and chloramphenicol, but resistance to penicillin, amikacin and cefradine. A resistance gene fragment about 1,900bp in the length of BCT gene was amplified by PCR, which had a 99% homology with BCT gene sequences in GenBank. These results proved that the isolate was B.trehalosi. The isolation and identification of the pathogenic B.trehalosi will facilitate study of the pathogenic mechanism and provide reference for the clinical drug usage.

参考文献/References:

[1]Sneath P H, Stevens M. Actinobacillus rossii sp. nov. Actinobacillus seminis sp. nov. nom. rev. Pasteurella bettii sp. nov. Pasteurella lymphangitidis sp. nov. Pasteurella mairi sp. nov. and Pasteurella trehalosi sp. nov. [J]. Int J Syst Bacteriol, 1990, 40(2): 148-150.
[2]Keller P, Müller C, Engelhardt I, et al. An antifungal benzimidazole derivative inhibits ergosterol biosynthesis and reveals movel sterols [J]. Antimicrob Agents Chemother, 2015, 59(10): 6296- 6307.
[3]Blackall P J, Bojesen A M, Christensen H, et al. Reclassification of [Pasteurella] trehalosi as Bibersteinia trehalosi gen. nov. comb. nov. [J]. Int J Syst Evol Micr, 2007, 57(Pt 4): 666-680.
[4]Spagnoli S, Reilly T J, Calcutt M J, et al. Bibersteinia trehalosi RNA polymerase beta subunit (rpoB) gene, partial cds [J]. Vet Patho, 2011, 49(5): 775-778.
[5]Machino M. Pasteurella haemolytica osteomyelitis in the cervical spine; a case report [J]. Jap J Orthop Surg Tr, 2007, 50: 839-840.
[6]汪斌,梁慧,韩彬等海藻巴斯德菌感染导致脑脓肿1例[J]. 中国人兽共患病学报,2007,23(1):98-100.
[7]何琼. 溶血性巴斯德菌致面痈1例[J]. 中国皮肤性病学杂志,2012,26(8):732.
[8]何银华,方芳,金立钢,等. 溶血巴斯德菌致泌尿系感染1例报道[J]. 中华医院感染学杂志,2008,18(12):1666.
[9]常璠. 溶血巴斯德菌生物T型致肺部感染1例[J]. 中华医院感染学杂志,2010,20(23):3786.
[10]蒙正群,冷依伊,任梅渗,等. 一株牛源肺炎克雷伯氏菌的分离鉴定与耐药基因型检测[J]. 浙江农业学报,2017,29(4):534-541.
[11]Greisen K, Loeffelholz M, Purohit A, et al. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid [J]. J Clin Microbiol, 1994, 32(2): 335-351.
[12]李颖利. 上海市活禽市场弯曲菌耐药性调查及其分子耐药机制的初步研究[D]. 中国农业科学院,2014.
[13]Lévesque C, Piché L, Larose C, et al. PCR mapping of integrons reveals several novel combinations of resistance genes [J]. Antimicrob Agents Chemother, 1995, 39(1): 185-191.
[14]林星宇,王印,杨泽晓,等. 猪源肺炎克雷伯菌的分离鉴定[J]. 中国预防兽医学报,2015,37(05):375-378.
[15]Ackermann M R, Brogden K A. Response of the ruminant respiratory tract to Mannheimia (Pasteurella) haemolytica [J]. Microbes Infect, 2000, 2(9): 1079-1088.
(本文编辑:李 爽)

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更新日期/Last Update: 2018-07-12