[1]许腾林,邢桂玲,姜成刚,等.猪、鸡与貂源肠外致病性大肠杆菌群型、耐药性与致病性分析[J].中国预防兽医学报,2018,(05):386-391.[doi:10.3969/j.issn.1008-0589.201712017]
 XU Teng-lin,XING Gui-ling,JIANG Cheng-gang,et al.Phylogenetic group, antimicrobial resistance and pathogenic analysis of extraintestinal pathogenic Escherichia coli isolated from swine, chicken and mink[J].Chinese journal of preventive veterinary medicine,2018,(05):386-391.[doi:10.3969/j.issn.1008-0589.201712017]
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猪、鸡与貂源肠外致病性大肠杆菌群型、耐药性与致病性分析()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年05
页码:
386-391
栏目:
病原生物学
出版日期:
2018-05-15

文章信息/Info

Title:
Phylogenetic group, antimicrobial resistance and pathogenic analysis of extraintestinal pathogenic Escherichia coli isolated from swine, chicken and mink
文章编号:
1008-0589(2018)5-0386-06
作者:
 

许腾林12邢桂玲23姜成刚2刘家森2刘大飞2姜 骞2李志杰2康洪涛2郭东春2*曲连东2*

 (1. 黑龙江八一农垦大学 动物科技学院,黑龙江 大庆163319;2. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室,黑龙江 哈尔滨150069;3. 东北农业大学 动物科技学院,黑龙江 哈尔滨 150069)
Author(s):
 

XU Teng-lin12 XING Gui-ling23 JIANG Cheng-gang2 LIU Jia-sen2 LIU Da-fei2 JIANG Qian2LI Zhi-jie2 KANG Hong-tao2 GUO Dong-chun2* QU Lian-dong2*

 (1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
2. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Institute, Chinese Academy of Agricultural Sciences,
Harbin 150069, China; 3. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150069, China)
关键词:
肠外致病性大肠杆菌分离鉴定耐药性致病性
Keywords:
 extraintestinal pathogenic Escherichia coli  isolation and identification  antimicrobial resistance  pathogenicity
分类号:
S852.61
DOI:
10.3969/j.issn.1008-0589.201712017
文献标志码:
A
摘要:
为检测不同动物源肠外致病性大肠杆菌(ExPEC)的耐药及致病性差异,本研究从132份鸡、猪和水貂等动物肠道外组织中分离鉴定大肠杆菌,并对分离菌株进行了16S rRNA基因测序、毒力基因检测、药敏试验、O抗原鉴定、系统进化群分析及动物致病性试验。实验结果显示:分离出53株ExPEC,且至少携带特异性毒力基因pap、sfa/foc、afa/dra、iutA、kpsMT II中的两种基因,并携带fimH、ibeA、hlyA、malX、iss、iroN等其它相关毒力基因。53株ExPEC分离株对氯霉素、卡那霉素、头孢噻吩、四环素、多粘菌素B、氨苄青霉素等呈现不同程度的多重耐药性;O抗原主要分布于O1、O2、O8、O11、O38、O78、O120血清型;系统进化群分析结果显示:28株属于A群,19株属于B1群,1株为B2群,5株属于D群。选取20株ExPEC分离菌株按照6×106 cfu/只对小鼠进行腹腔注射,验证其致病性;并测定ExPEC6、ExPEC42、ExPEC52、ExPEC53菌株对BALB/c小鼠的LD50,其结果分别为3.49×106 cfu/mL、9.49×105 cfu/mL、9.48×106 cfu/mL、2.38×107 cfu/mL。本研究为动物源性ExPEC的预防诊断提供依据,并为下一步研究其致病机理等奠定基础。
Abstract:
To examine the antimicrobial resistance and pathogenicity differences of extraintestinal pathogenic Escherichia coli(ExPEC) from different animals, the ExPEC were isolated and identified from 132 extra-intestinal tissues of infected animals, such as swine, chicken and mink. Then these ExPEC were subjected to examination of the 16S rRNA gene, virulence genes, antimicrobial resistance, O-antigen, phylogenetic classification, and pathogenicity to mice. The results showed that 53 of ExPEC isolates possessed at least two specific virulence genes of pap, sfa/foc, afa/dra, iutA, kpsMT II, and carried other virulence factorgenes such as fimH, ibeA, hlyA, malX, iss, iroN and so on. The 53 ExPEC displayed different multiple antimicrobial resistances to Chloramphenicol, Kanamycin, Cefotaxime, Tetracycline, Ampicillin, Polymyxin B and so on. The serogroups of O-antigen were mainly distributed in O1, O2, O8, O11, O38, O78 and O120. Phylogenetic analysis showed that the classifications included group A of 28 isolates, group B1 of 19 isolates, group B2 of 1 isolates and groupD of 5 isolates. The pathogenicity of 20 ExPEC isolates was determined by intraperitoneally injecting mice at dose of 6×106 cfu/mice. Then the LD50 of ExPEC6, ExPEC42, ExPEC52, ExPEC53 isolates in mice were 3.49×106 cfu/mL, 9.49×105 cfu/mL, 9.48×106 cfu/mL and 2.38×107 cfu/mL, respectively. This study provided a basis for the prevention and diagnosis of animal ExPEC, and the foundation to further study bacteria pathogenesis.

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(本文编辑:李 爽)

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更新日期/Last Update: 2018-05-15