[1]郭 晶,李媛媛,裴兰英,等.H7N9流感病毒人源分离株与禽源分离株复制特性研究[J].中国预防兽医学报,2018,(05):371-374.[doi:10.3969/j.issn.1008-0589.201708023]
 GUO Jing,LI Yuan-yuan,PEI Lan-ying,et al.Investigation of the replication property of H7N9 human and chicken isolates in cell cultures[J].Chinese journal of preventive veterinary medicine,2018,(05):371-374.[doi:10.3969/j.issn.1008-0589.201708023]
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H7N9流感病毒人源分离株与禽源分离株复制特性研究()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年05
页码:
371-374
栏目:
病原生物学
出版日期:
2018-05-15

文章信息/Info

Title:
Investigation of the replication property of H7N9 human and chicken isolates in cell cultures
文章编号:
1008-0589(2018)5-0371-04
作者:
 

郭 晶1李媛媛2裴兰英1邓国华2施建忠2陈化兰2*李旭勇1*

 (1. 聊城大学 农学院,山东 聊城 252000;
2. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室,黑龙江 哈尔滨 150069)
Author(s):
 

GUO Jing1 LI Yuan-yuan2 PEI Lan-ying1 DENG Guo-hua2 SHI Jian-zhong2 CHEN Hua-lan2* LI Xu-yong1*

 (1. College of Agricultural, Liaocheng University, Liaocheng 252000, China; 2. State Key Laboratory of Veterinary Biotechnology,
Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China)
Keywords:
  H7N9  influenza virus  replication  cell culture
分类号:
S852.65
DOI:
10.3969/j.issn.1008-0589.201708023
文献标志码:
A
摘要:
为了解H7N9流感病毒的生物学特性,本研究对H7N9流感病毒人源分离株(AH1)和禽源分离株(CK53)复制特性进行了系统比较研究。序列分析显示,AH1和CK53病毒在进化关系上同源性较高,仅有8个氨基酸位点差异。两株病毒分别接种禽类细胞DF1和CEF,人类细胞A549和Calu-3,检测其复制能力,结果显示在33℃和37℃两种温度条件下,两种病毒在禽类细胞中复制能力相似,但在人类细胞中AH1复制能力强于CK53。CK53在哺乳动物细胞连续培养5代后出现包含PB2/E627K在内共5个氨基酸位点的突变,显示禽源分离株在哺乳动物细胞复制过程中可以迅速获得适应性突变。突变病毒CK53-P5在A549细胞中复制水平明显强于CK53,其复制能力与AH1差异不明显。本研究进一步揭示了H7N9流感病毒人源分离株与禽源分离株的生物学特性差异,为深入了解其致病分子机制提供了实验依据。
  关键词:H7N9;流感病毒;复制;细胞
Abstract:
H7N9 influenza viruses have posed great threat to the poultry industry and human public health since the first epidemic of H7N9 in 2013. To further investigate the biological characteristics of H7N9 viruses, the replication characteristics of two H7N9 influenza viruses isolated from human (AH1) and avian (CK53) were systematically compared in avian and human cells. Only eight amino acids were different between AH1 and CK53. The two viruses showed similar replication ability in DF1 and CEF cells at 33℃ and 37 ℃. However, AH1 was able to replicate to a higher titer than CK53 in A549 and Calu-3 cells at 33 ℃ and 37 ℃. Five amino acids mutations were detected in CK53 after serial passage in mammal cells, which implied that the avian originated H7N9 virus was able to quickly acquire the adaptive mutations. In addition, the viral titers of the adaptive virus CK53-P5 was higher than that of the CK53 virus in A549 cells, but no significant difference was observed in the viral replication ability between CK53-P5 and AH1. This study revealed the diversity of the human and avian H7N9 viruses in their biological properties, and provided useful information for the further investigation in the molecular mechanism of pathogenicity of H7N9 viruses.

参考文献/References:

[1] Shi Jian-zhong, Deng Guo-hua, Liu Pei-hong, et al. Isolation and characterization of H7N9 viruses from live poultry markets - Implication of the source of current H7N9 infection in humans [J]. Chin Sci Bullet, 2013, 58: 1857-1863.
[2]Zhang Qian-yi, Shi Jian-zhong, Deng Guo-hua, et al. H7N9 influenza viruses are transmissible in ferrets by respiratory droplet [J]. Science, 2013, 341: 410-414.
[3]Zhou Jiang-fang, Wang Da-yan, Gao Rong-bao, et al. Biological features of novel avian influenza A (H7N9) virus [J]. Nature, 2013, 499: 500-503.
[4]Watanabe T, Kiso M, Fukuyama S, et al. Characterization of H7N9 influenza A viruses isolated from humans [J]. Nature, 2013, 501: 551-555.
[5]Zhang Hong, Li Xu-yong, Guo Jing, et al. The PB2 E627K mutation contributes to the high polymerase activity and enhanced replication of H7N9 influenza virus [J]. J Gen Virol, 2014, 95:779-786.
[6]Belser J A, Gustin K M, Pearce M B, et al. Pathogenesis and transmission of avian influenza A (H7N9) virus in ferrets and mice [J]. Nature, 2013, 501: 556-559.
[7]Mok C K, Lee H H, Chan M C, et al. Pathogenicity of the novel A/H7N9 influenza virus in mice [J]. MBio, 2013, 4(4): e00362-13.
                                      (本文编辑:彭永刚)

更新日期/Last Update: 2018-05-15