[1]靳元春,刘进辉,孔小鲜,等.基于ITS基因分析蛇源裂头蚴种系进化关系[J].中国预防兽医学报,2018,(02):167-169.[doi:0.3969/j.issn.1008-0589.201705022]
 JIN Yuan-chun,LIU Jin-hui,KONG Xiao-xian,et al.Phylogenetic analysis of ribosomal ITS gene of snake Sparganum in Hunan province[J].Chinese journal of preventive veterinary medicine,2018,(02):167-169.[doi:0.3969/j.issn.1008-0589.201705022]
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基于ITS基因分析蛇源裂头蚴种系进化关系()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年02
页码:
167-169
栏目:
研究简报
出版日期:
2018-02-15

文章信息/Info

Title:
Phylogenetic analysis of ribosomal ITS gene of snake Sparganum in Hunan province
文章编号:
1008-0589(2018)2-0167-03
作者:
 

靳元春刘进辉孔小鲜谭 磊刘 伟*

 (湖南农业大学 动物医学院,湖南 长沙 410128)
Author(s):
 

JIN Yuan-chun LIU Jin-hui KONG Xiao-xian TAN Lei LIU Wei*

 (College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China)
Keywords:
 snake Sparganum  ribosomal rDNA  ITS  phylogenetic relationship
分类号:
S852.7
DOI:
0.3969/j.issn.1008-0589.201705022
文献标志码:
A
Abstract:
To explore the phylogenetic relationship of snake Sparganum, the gene sequence of internal transcribed spacers(ITS) in ribosomal DNA of snake Sparganum isolated from Hunan was amplified and sequenced. Then, ITS gene sequences alignment, and phylogenetic tree analysis were performed. The results showed that the lengths of the amplicated ITS gene sequences from the 12 snake Sparganums isolated from Hunan were 1 386 bp-1 404 bp, and the differences among the isolates were small. The differences of the corresponding gene sequences among the isolates were 0.2%-1.3% (ITS-1) and 0.4%-2.2% (ITS-2), respectively. The nucleotide similarities of Hunan isolates with Taeniidae reference strains on GenBank were lower than 66.3% (ITS-1) and 52.1% (ITS-2), which indicated the ITS gene sequences of the snake Sparganum were relatively conservative in the species, but the differences between species were great, and could be used as a genetic marker for species genetic variation of snake Sparganum. The constructed phylogenetic trees based on the ITS gene sequences revealed that the Hunan isolates and the Spirometra erinacei-europaei available in GenBank were clustered in the same clade, which indicated the Hunan isolates all belonged to the Spirometra erinacei-europaei. This study provides reference basis for identification, diagnosis, and molecular epidemiology survey of snake Sparganum.

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更新日期/Last Update: 2018-03-12