[1]田佳琪,董文龙,王 巍,等.猪源解鸟氨酸拉乌尔菌分离鉴定与大环内酯耐药基因检测[J].中国预防兽医学报,2018,(01):1-04.[doi:0.3969/j.issn.1008-0589.201703055]
 TIAN Jia-qi,DONG Wen-long,WANG Wei,et al.Isolation and identification of Raoultella ornithinolytica and detection of macrolide resistance gene[J].Chinese journal of preventive veterinary medicine,2018,(01):1-04.[doi:0.3969/j.issn.1008-0589.201703055]
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猪源解鸟氨酸拉乌尔菌分离鉴定与大环内酯耐药基因检测()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2018年01
页码:
1-04
栏目:
病原生物学
出版日期:
2018-01-15

文章信息/Info

Title:
Isolation and identification of Raoultella ornithinolytica and detection of macrolide resistance gene
文章编号:
1008-0589(2018)1-0001-04
作者:
 

田佳琪董文龙王 巍王 羽张宏伟张喜庆李雪嵩马红霞高云航*

 (吉林农业大学 动物科学技术学院,吉林 长春 130118)
Author(s):
 

TIAN Jia-qi DONG Wen-long WANG Wei WANG Yu ZHANG Hong-wei ZHANG Xi-qingLI Xue-song MA Hong-xia GAO Yun-hang*

 (College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China)
关键词:
解鸟氨酸拉乌尔菌分离鉴定药敏试验大环内酯耐药基因
Keywords:
Raoultella ornithinolytica  Isolated and identified  drug resistance  macrocyclic resistance gene
分类号:
S852.4
DOI:
0.3969/j.issn.1008-0589.201703055
文献标志码:
A
摘要:
为确定吉林省某养殖场导致猪群死亡的病原菌,本研究从病死猪胸腔积液中分离得到一株优势菌,经16S rDNA检测该株优势菌为解鸟氨酸拉乌尔菌;动物回归试验显示分离株对猪、小鼠均有致病性;药敏试验结果显示该菌对大环内酯类的麦迪霉素、阿奇霉素、红霉素、泰乐菌素呈显著耐药,并通过PCR检测到分离菌株的大环内酯耐药基因呈显性表达。本研究首次从猪体内分离到解鸟氨酸拉乌尔菌,并为该条件致病菌的临床诊疗、免疫防控提供参考依据。
  
Abstract:
To investigate the pathogenic bacteria caused the swine dead in Jilin provience, the dominant bacteria was isolated from pleural effusion, and detected as Raoultella orithinolytica by 16S rDNA PCR amplification and sequencing. The result of animal regression experiment showed the isolate had pathogenicity to swine and mice. The result of minimum inhibitory concentration(MIC) showed that the isolated bacteria was resistant to maddie azithromycin, erythromycin and tylosin, and macrocyclic resistant gene of Raoultella orithinolytica was detected by PCR. The study was the first time to isolate Raoultella orithinolytica from swine, and the experiment data could provide the reference basis for clinical treatment and immune prevention of the conditional pathogen.

参考文献/References:

[1] Seng Piseth, Boushab B M, Romain F, et al. Emerging role of Raoultella ornithinolytia in human infections: A series of cases and review of literature [J]. Int J Comput Appl Infect Dis, 2016, 45: 65-71.
[2]Kaya S, Bayramoglu G, Sonmez M, et al. Raoultella ornithinolytica, causing fatal sepsis [J]. Braz J Infect Dis, 2015, 51(2):230-231.
[3]Sekowska A, Dylewska K, Gospodarek E, et al. Catheter-related blood stream infection caused by Raoultella ornithinolytica [J]. Folia Microbiol, 2015, 60(6): 1-3.
[4]池丹,胡燕燕,马继华,等. 一株解鸟氨酸拉乌尔菌的鉴定及其β内酰胺酶编码基因的研究[J]. 中华检验医学杂志,2013,36(11):1045-1047.
[5]董杰. 细菌获得性抗生素耐药基因研究进展[J]. 中国预防医学杂志,2015,16(1):71-74.
[6]Levorova J, Machon V, Guha A, et al. Septic arthritis of the temporomandibular joint caused by rare bacteria Raoultella ornithinolytica [J]. Int J Oral Maxillofac Surg, 2017, 46(1): 111- 115.

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更新日期/Last Update: 2018-02-07