[1]郝俊玺,董志民,王秋东,等.高寒地区奶牛乳房炎凝固酶阴性致病葡萄球菌的分离鉴定及耐药性分析[J].中国预防兽医学报,2017,(10):848-851.[doi:10.3969/j.issn.1008-0425.201706009]
 HAO Jun-xi,DONG Zhi-min,WANG Qiu-dong,et al.Identification and antimicrobial resistance of coagulase-negative Staphylococcus of mastitis in alpine region[J].Chinese journal of preventive veterinary medicine,2017,(10):848-851.[doi:10.3969/j.issn.1008-0425.201706009]
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高寒地区奶牛乳房炎凝固酶阴性致病葡萄球菌的分离鉴定及耐药性分析()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2017年10
页码:
848-851
栏目:
出版日期:
2017-10-15

文章信息/Info

Title:
Identification and antimicrobial resistance of coagulase-negative Staphylococcus of mastitis in alpine region
文章编号:
1008-0589(2017)10-0848-04
作者:
 

郝俊玺1董志民34王秋东5华 欣2祝 瑶2杨 亲2鄢明华34刘思国2张万江2*

 (1. 内蒙古农业大学,内蒙古 呼和浩特 010018;2. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/动物细菌病研究室,黑龙江 哈尔滨 150069;3. 天津市畜牧兽医研究所,天津 西青 300381;4. 天津市畜禽健康养殖技术工程中心,天津 西青 300381;5. 吉林农业大学 动物科学技术学院,吉林 长春 130118)
Author(s):
 

HAO Jun-xi1 DONG Zhi-min34 WANG Qiu-dong5 HUA Xin2 ZHU Yao2 YANG Qin2 YAN Ming-hua34 LIU Si-guo2 ZHANG Wan-jiang2*

 (1. Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010080, China; 2. Division of Bacterial Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China;
3. Tianjin Animal Science and Veterinary Research Institute, Tianjin 300381, China; 4. Tianjin livestock and poultry health breeding
technology Engineering Center,Tianjin 300381, China; 5. College of animal science and technology, Jilin Agricultural University,
Changchun 130118, China)
关键词:
 葡萄球菌乳房炎药敏试验PFGE分型
分类号:
S852.61
DOI:
10.3969/j.issn.1008-0425.201706009
文献标志码:
A
摘要:
为调查高寒牧区引起奶牛乳房炎的凝固酶阴性致病葡萄球菌流行现状及耐药情况,本研究对呼伦贝尔地区3个规模化奶牛养殖场采用加州乳房炎测试法(CMT)进行高体细胞牛脂病检测,并采集330份牛奶样品,应用PCR方法、脉冲场凝胶电泳(PFGE)分型和药敏试验等对样品中的葡萄球菌进行分离鉴定和耐药性分析。结果显示,该地区隐性乳房炎发病率达47.8 %,共分离和鉴定出9种葡萄球菌60株(34.5 %);60株葡萄球菌分为41个克隆谱型;60株葡萄球菌对青霉素和林可霉素的耐药率较高,分别为40.0 % (24/60)和50.0 % (30/60),表明这两种药是治疗乳房炎常用药物。本研究为高寒地区致病葡萄球菌引起的奶牛乳房炎临床合理用药及对病原菌的防控提供实验依据和指导。
 
Abstract:
 
To investigate the prevalence and drug resistance of coagulase-negative Staphylococcus from mastitis of dairy cow in Hulunbuir, a total of 330 milk samples were collected from dairy cows with a high somatic cell disease tallow (using the California mastitis test method, CMT) from three large-scale farms, during the period May to September 2015. Staphylococcus spp strains were isolated and identified by PCR, pulsed field gel electrophoresis (PFGE) typing and antimicrobial susceptibility testing, respectively. The results showed that 60 Staphylococcus spp strains belonging to 9 different genera and 41 clone-types were obtained, and the isolation rate was 47.8%. The 13 different genera Staphylococcus included 4 main pathogenic staphylococci (114 strains, 65.5%) and 9 opportunistic Staphylococci (60 strains, 34.5%). These Staphylococcus isolates showed resistant rate to

参考文献/References:

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                                                (本文编辑:彭永刚)

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更新日期/Last Update: 2017-11-06