[1]姜雪婷,黄立平,王 伟,等.利用ICR小鼠模型对PCV2感染中TLR3信号通路的研究[J].中国预防兽医学报,2017,(10):820-825.[doi:10.3969/j.issn.1008-0425.201701006]
 JIANG Xue-ting,HUANG Li-ping,WANG Wei,et al.Signal pathway of TLR3 induced by Procine circovirus type 2 in spleen of ICR mice[J].Chinese journal of preventive veterinary medicine,2017,(10):820-825.[doi:10.3969/j.issn.1008-0425.201701006]
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利用ICR小鼠模型对PCV2感染中TLR3信号通路的研究()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2017年10
页码:
820-825
栏目:
免疫学
出版日期:
2017-11-15

文章信息/Info

Title:
Signal pathway of TLR3 induced by Procine circovirus type 2 in spleen of ICR mice
文章编号:
1008-0589(2017)10-0820-06
作者:
 

姜雪婷12黄立平2王 伟2危艳武2刘赛宝2鲁国涛2刘长明2*孟庆文2*陈洪岩12*

 (1. 东北农业大学 生命科学学院,黑龙江 哈尔滨 150030;
2. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室,黑龙江 哈尔滨150069)
Author(s):
 

JIANG Xue-ting12 HUANG Li-ping2 WANG Wei2 WEI Yan-wu2 LIU Sai-bao2 LU Guo-tao2 LIU Chang-ming2* MENG Qing-wen2* CHEN Hong-yan12*

 (1. College of life science, Northeast Agricultural University, Harbin 150030, China
2. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China)
关键词:
PCV2感染ICR小鼠TLR3信号通路细胞因子
分类号:
S852.61
DOI:
10.3969/j.issn.1008-0425.201701006
文献标志码:
A
摘要:
为探究猪圆环病毒2型(PCV2)感染对TLR3信号通路及炎性细胞因子的影响,本研究将30只6周龄ICR雌鼠随机分成两组(感染组和对照组),腹腔接种PCV2b/YJ株,在接种后第7 d、14 d、21 d、28 d和35 d采集小鼠外周血及脾组织。以临床观察、脾组织PCV2基因拷贝、小鼠血清抗体效价、IL-10和TNF-α的mRNA水平等指标评估感染模型;荧光定量PCR检测小鼠脾组织TLR3、TRIF、IFN-β、Mx1的mRNA水平,western blot检测TLR3蛋白表达水平。结果显示:PCV2感染期间感染组小鼠无可见的临床症状,第7 d至35 d感染组小鼠脾组织PCV2基因拷贝数呈逐渐降低趋势,PCV2的IPMA血清抗体在感染后第7 d转阳、35 d达到最高,IL-10 mRNA在感染后第14 d起升高、维持高水平;TNF-α mRNA在感染后第21 d和28 d升高。在感染后第7 d和14 d感染组小鼠TLR3、TRIF mRNA和TLR3蛋白表达水平显著升高;IFN-β mRNA在感染后第7 d具有显著升高过程;Mx1 mRNA在感染后第14 d时有上调过程。本实验在建立PCV2b/YJ亚临床感染ICR小鼠模型的基础上,体内实验表明PCV2感染小鼠激活了TLR3信号通路。本研究为利用敲除小鼠模型阐明TLR3参与PCV2感染引发的免疫应答机制奠定了基础。
  
Abstract:
To investigate the effect of porcine circovirus type 2 (PCV2) infection on TLR3 signaling pathways and inflammatory cytokines, 30 ICR mice were divided into two groups randomly (experiment group and control group), experiment group was inoculated with PCV2 via the intraperitoneal route, and peripheral blood and spleen of the mice were collected at 7, 14, 21, 28 and 35 days post infection (dpi). The infection model was assessed by clinical signs, DNA copies of the PCV2 in spleen, antibody titers in serum, and the mRNA transcription of IL-10 and TNF-α in mice. The mRNA transcript levels of TLR3, TRIF, IFN-β and Mx1 in spleen of the mice were detected by quantitative real-time RT-PCR, and the protein expression of TLR3 in mice was measured by western blot. Results showed that no obvious clinical signs compatible with PCV2 were observed on ICR mice. The copy number of PCV2 gene in spleen tissue of 7dpi to 35dpi tended to decrease gradually. Antibody of mice against PCV2

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(本文编辑:李 爽)

更新日期/Last Update: 2017-11-06