[1]孔兴天,杨孝朴,关立峥,等.两株H9N2亚型禽流感病毒的全基因组序列测定及其对小鼠的感染性研究[J].中国预防兽医学报,2017,(10):779-783.[doi:10.3969/j.issn.1008-0425.201703002]
 KONG Xing-tian,YANG Xiao-pu,GUAN Li-zheng,et al.The complete genomic sequencing of two H9N2 avian influenzaviruses and their infectivity in mice[J].Chinese journal of preventive veterinary medicine,2017,(10):779-783.[doi:10.3969/j.issn.1008-0425.201703002]
点击复制

两株H9N2亚型禽流感病毒的全基因组序列测定及其对小鼠的感染性研究()
分享到:

《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2017年10
页码:
779-783
栏目:
出版日期:
2017-10-15

文章信息/Info

Title:
The complete genomic sequencing of two H9N2 avian influenzaviruses and their infectivity in mice
文章编号:
1008-0589(2017)10-0779-05
作者:
 

孔兴天12杨孝朴1关立峥12崔鹏飞2邓国华2*陈化兰12*

 (1. 甘肃农业大学 动物医学院,甘肃 兰州 730070;2. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点试验室/
农业部动物流感重点开放试验室,黑龙江 哈尔滨 150069)
Author(s):
 

KONG Xing-tian12 YANG Xiao-pu1 GUAN Li-zheng12 CUI Peng-fei2 DENG Guo-hua2* CHEN Hua-lan12*

 (1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2. Animal Influenza Key Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotecnology, Harbin Veterinary Research Insitute,
Chinese Academy of Agricultural Sciences, Harbin 150069, China)
关键词:
禽流感病毒H9N2亚型进化分析感染性
Keywords:
 avian influenza virus  H9N2 subtype  phylogenetic analysis  infection
分类号:
S852.65
DOI:
10.3969/j.issn.1008-0425.201703002
文献标志码:
A
摘要:
 为了解2015年广西地区活禽市场中的两株鸡源H9亚型禽流感病毒(AIV) A/chicken/GX/S30272/2015(H9N2) (简称GX/272/15株)和A/chicken/GX/S30825/2015 (H9N2) (GX/825/15株)的生物学特性,本研究对其进行了全基因组序列测定和小鼠的感染性试验。核苷酸序列分析表明,两株病毒的HA基因发生了I155T、H183N、A190T和Q226L突变,这些突变与增强病毒的人源受体结合特性密切相关。遗传演化关系表明,两株病毒的HA基因均属于欧亚分支Beijing/1/94谱系。GX/272/15株的8个基因节段和GX/825/15株的HA节段来源于H9N2 AIV,GX/825/15株的其余7个基因节段分别来源于H4N2、H10N6、H7N9、H7N1、H9N2、H6N6和H3N2多种不同亚型的AIV。两株病毒均可感染小鼠,且均不需要经过适应即可在小鼠的鼻甲内进行复制。但仅GX/825/15可以在小鼠的肺脏内复制。两株病毒均未造成小鼠死亡,仅引起小鼠体重的轻微变化,对小鼠呈低致病力。本研究结果表明,H9N2亚型AIV能够感染哺乳动物,应加强后续分离病毒对人类健康危害性的评估。
  
Abstract:
To investigate the biological characteristics of two H9N2 subtype avian influenza viruses (AIVs) of GX/272/15 and GX/825/15 isolated from chickens in live poultry markets in Guangxi in 2015, the whole genomic sequence were analyzed and the infectivity was evaluated in BALB/cmice. The nucleotide sequence analyses found that the mutations of I155T, H183N, A190T and Q226L occurred in HA of the two isolates, which were closely related to enhancing human-type receptor binding specificity of the viruses in HA. Phylogenetic relationships indicated that the HA genes of the two isolates belonged to Beijing/1/94 sublineage of the Eurasian lineage. The eight segments of GX/272/15 and the HA genes of GX/825/15 originated from the H9 AIVs, the other seven segments of GX/825/15 derived from diverse subtypes viruses including H4N2, H10N6, H7N9, H7N1, H9N2, H6N6 and H3N2 AIVs. In addition, both the viruses were able to infect mice and replicate in the upper respiratory tract of infected mice without adaptation. However, only GX/825/15 could be detected in the lungs of mice. The two viruses did not cause the death of mice, only caused slight changes in body weight of mice during the observation period, indicating that the two HPN2 viruses were low pathogenicity to mice. These results demonstrated that the H9N2 AIVs had the ability to infect mammals and the potential harmfulness of H9N2 subtype AIVs to human health need to be further evaluated.
  

参考文献/References:

[1] Hoffmann E, Stech J, Guan Y, et al. Universal primer set for the full-length amplification of all influenza A viruses[J]. Arch Virol, 2001, 146(12): 2275-2289.
[2]张芳,王晶,卢昆鹏,等.一株野鸟源 H9N2 亚型AIV的全基因组序列分析及致病性研究[J].中国预防兽医学报,2015, 37(1):15-18.
[3]Sorrell E M, Wan Hong-quan, Araya Y, et al. Minimal molecular constraints for respiratory droplet transmission of an avian- human H9N2 influenza A virus [J]. PNAS USA, 2009, 106(18): 7565-7570.
[4]Wan Hong-quan, Sorrell E M, Song Hai-chen, et al. Replication and transmission of H9N2 influenza viruses in ferrets: evaluation of pandemic potential [J]. PLoS One, 2008, 3(8): e2923.
[5]Li Cheng-jun, Yu Kang-zhen, Tian Guo-bin, et al. Evolution of H9N2 influenza viruses from domestic poultry in Mainland China [J]. Virology, 2005, 340(1): 70-83.
[6]Matrosovich M N, Krauss S, Webster R G. H9N2 Influenza A viruses from poultry in Asia have human virus-like receptorspecificity [J]. Virology, 2001, 281(2): 156-162.
[7]Jia Na, Liu Yun-xi, Zhang Jiu-song, et al. Serological reports of human infections of H7 and H9 avian influenza viruses innorthern China [J]. J Clin Virol, 2009, 44(3): 225-229.
[8]Li Xu-yong, Shi Jian-zhong, Chen Hua-lan, et al. Genetics, receptor binding property and transmissibility in mammals of naturally isolated H9N2 avian influenza viruses [J]. PLoS Pathog,2014, 10(11): 1-12.
[9]Shaw M, Gregory V, Cameron K, et al. Avian-to-human transmission of H9N2 subtype influenza A viruses: Relationship between H9N2 and H5N1 human isolates [J]. PNAS USA, 2000, 97(17): 9654-9658.
[10]Yu Xin-fen, Jin Tao, Cui Yu-jun, et al. Influenza H7N9 and H9N2 viruses: coexistence in poultry linked to human H7N9 infection and genome characteristics [J]. J Virol, 2014, 88(6):3423-3431.
[11]Deng Guo-hua, Shi Jian-zhong, Wang Jing, et al. Genetics, receptor binding, and virulence in mice of H10N8 influenza viruses isolated from ducks and chickens in live poultry markets in China [J]. J Virol, 2015, 89(12): 6506-6510.
[12]Gao Rong-bao, Cao Bin, Hu Yun-wen, et al. Human infection with a novel avian-origin influenza A (H7N9) virus [J]. N Engl J Med, 2013, 368(20): 1888-1897.
[13]Liu Ming-bin, Li Xiao-dan, Yuan Hui, et al.Genetic diversity of avian influenza A (H10N8) virus in live poultry markets and its association with human infections in China [J]. Sci Rep, 2015, 5: 7632.
                                            (本文编辑:彭永刚)

相似文献/References:

[1]葛 叶,邓国华*,李雪平,等.流感病毒聚合酶PA亚单位抗血清的制备[J].中国预防兽医学报,2009,(04):314.
 GE Ye,DENG Guo-hua*,LI Xue-ping,et al.Preparation of polyclonal antibody of avian influenza virus polymerase PA subunit[J].Chinese journal of preventive veterinary medicine,2009,(10):314.
[2]张 莹,高玉伟,邓国华,等.不同H5N1亚型禽流感病毒在豚鼠体内的复制和传播能力评估[J].中国预防兽医学报,2010,(03):167.
 ZHANG Ying,GAO Yu-wei,DENG Guo-hua,et al.Evaluation of replication and transmissibility of different H5N1 avian influenza virus in guinea pigs[J].Chinese journal of preventive veterinary medicine,2010,(10):167.
[3]郭莹莹,吴春艳,王靖飞*.禽流感分离株GD/1/96(H5N1)非结构蛋白结构建模[J].中国预防兽医学报,2010,(03):171.
 GUO Ying-ying,WU Chun-yan,WANG Jing-fei*.Structure Modeling of the nonstructural protein from A/Goose/Guangdong/1/96 (H5N1) strain of avian influenza virus[J].Chinese journal of preventive veterinary medicine,2010,(10):171.
[4]刘丽玲,李雁冰*,施建忠,等.一株鸡源H6N1亚型禽流感病毒全基因的分子特征[J].中国预防兽医学报,2010,(03):214.
 LIU Li-ling,LI Yan-bing*,SHI Jian-zhong,et al.Molecular characterizations of a isolated from chicken H6N1 subtype avian influenza virus[J].Chinese journal of preventive veterinary medicine,2010,(10):214.
[5]万晓朋,曾显营,田国彬*,等.H9N2亚型禽流感流行株灭活疫苗种毒的筛选[J].中国预防兽医学报,2010,(04):276.
 WAN Xiao-peng,ZENG Xian-ying,TIAN Guo-bin*,et al.Selection and evaluation of H9N2 avian influenza inactivate vaccine candidates[J].Chinese journal of preventive veterinary medicine,2010,(10):276.
[6]许 静#,宋家升#,苏胜杰,等.H5N1亚型禽流感病毒对鸭的致病性研究[J].中国预防兽医学报,2010,(04):301.
 XU Jing#,SONG Jia-sheng#,SU Sheng-jie,et al.Virulence and replication of H5N1 avian influenza viruses in domestic ducks[J].Chinese journal of preventive veterinary medicine,2010,(10):301.
[7]高宏博,崔红玉,石星明,等.表达H5亚型禽流感病毒HA基因重组火鸡疱疹病毒的构建[J].中国预防兽医学报,2010,(05):329.
 GAO Hong-bo,CUI Hong-yu,SHI Xing-ming,et al.Construction of recombinant turkey herpesvirus expressing haemagglutinin of H5 subtype of avian influenza virus[J].Chinese journal of preventive veterinary medicine,2010,(10):329.
[8]张 翼,段振华,刘丽玲,等.山西亚群H5N1亚型HPAIV对鸭及鸭胚的继代感染研究[J].中国预防兽医学报,2010,(05):365.
 ZHANG Yi,DUAN Zhen-hua,LIU Li-ling,et al.Successive passages of H5N1 highly pathogenic avian influenza virus clade SX in ducks and duck embryos[J].Chinese journal of preventive veterinary medicine,2010,(10):365.
[9]樊树芳,许传田,索永兵,等.H5N1亚型禽流感病毒A/Anhui/2/2005活候选疫苗株的构建及其对鸡致病性的研究[J].中国预防兽医学报,2008,(11):831.
 FAN Shu-fang,XU Chuan-tian,SUO Yong-bing,et al.Construction and pathogenicity studies of a live vaccine against highly pathogenic H5N1 avian influenza virus A/Anhui/2/2005[J].Chinese journal of preventive veterinary medicine,2008,(10):831.
[10]贾红玲,苏 艳,吴 润.禽流感病毒血凝素保守氨基酸对其受体结合位点的影响[J].中国预防兽医学报,2008,(07):500.
 JIA Hong-ling,SU Yan,WU Run.The Influence of the conservative amino acide of hemagglutinin to the receptor binding site of avian influenza virus[J].Chinese journal of preventive veterinary medicine,2008,(10):500.
[11]张 芳,王 晶,卢昆鹏,等.一株野鸟源H9N2亚型禽流感病毒的全基因组序列分析及致病性研究[J].中国预防兽医学报,2015,(01):15.[doi:10.3969/j.issn.1008-0589.2015.01.04]
 ZHANG Fang,WANG Jing,LU Kun-peng,et al.Whole genomic sequence and pathogenicity analysis of a wild bird-derived H9N2 subtype avian influenza virus[J].Chinese journal of preventive veterinary medicine,2015,(10):15.[doi:10.3969/j.issn.1008-0589.2015.01.04]
[12]肖 丽,崔鹏飞,张 芳,等.两株野鸟源H9N2亚型禽流感病毒的进化分析和对小鼠的感染性研究[J].中国预防兽医学报,2015,(11):817.[doi:10.3969/j.issn.1008-0589.2015.11.01]
 XIAO Li,CUI Peng-fei,ZHANG Fang,et al.Evolution and infectivity in mice of two wild bird-origin H9N2 subtype avian influenza viruses[J].Chinese journal of preventive veterinary medicine,2015,(10):817.[doi:10.3969/j.issn.1008-0589.2015.11.01]
[13]刘慧敏,蒋烈戈,王 时,等.一株鹌鹑源禽流感病毒A/quail/Jilin/7/2015(H9N2)的遗传进化分析和致病性研究[J].中国预防兽医学报,2017,(12):957.[doi:10.3969/j.issn.1008-0589.201709026]
 LIU Hui-min,JIANG Lie-ge,WANG Shi,et al.Phylogenetic analysis and pathogenicity of a quail-orgin avian influenza virus A/quail/Jilin/12/2015(H9N2)[J].Chinese journal of preventive veterinary medicine,2017,(10):957.[doi:10.3969/j.issn.1008-0589.201709026]
[14]袁小远,孟 凯,张玉霞,等.2016年~2017年山东地区商品肉鸡H9N2亚型禽流感病毒HA基因的分子特征和遗传进化分析[J].中国预防兽医学报,2018,(06):543.[doi:0.3969/j.issn.1008-0589.201708019]
 YUAN Xiao-yuan,MENG Kai,ZHANG Yu-xia,et al.Analysis on variation and molecular characteristics of hemagglutinin genes of H9N2 subtype avian influenza viruses isolated from Shandong in 2016-2017[J].Chinese journal of preventive veterinary medicine,2018,(10):543.[doi:0.3969/j.issn.1008-0589.201708019]

更新日期/Last Update: 2017-11-06