[1]任会玲,王雪峰,杜 承,等.马传染性贫血病毒诱导马胎皮细胞发生自噬的研究[J].中国预防兽医学报,2017,(09):708-711.[doi:10.3969/j.issn.1008-0425.2017.09.04]
 REN Hui-ling,WANG Xue-feng,DU Cheng,et al.Autophagy induced by equine infectious anemia virus in fetal equine dermal cells[J].Chinese journal of preventive veterinary medicine,2017,(09):708-711.[doi:10.3969/j.issn.1008-0425.2017.09.04]
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马传染性贫血病毒诱导马胎皮细胞发生自噬的研究()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2017年09
页码:
708-711
栏目:
病原生物学
出版日期:
2017-09-12

文章信息/Info

Title:
Autophagy induced by equine infectious anemia virus in fetal equine dermal cells
文章编号:
1008-0589(2017)09-0708-04
作者:
 

任会玲王雪峰杜 承刘 聪林跃智*王晓钧*

 (中国农业科学院哈尔滨兽医研究所 兽医生物技术重点实验室/马传染病与慢病毒病研究创新团队,黑龙江 哈尔滨 150069)
Author(s):
 

REN Hui-ling WANG Xue-feng DU Cheng LIU Cong LIN Yue-zhi* WANG Xiao-jun*

 (Division of Livestock Infectious Disease, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,
Chinese Academy of Agricultural Sciences, Harbin 150069, China)
关键词:
EIAV马胎皮细胞自噬LC3
Keywords:
EIAV  fetal equine dermal cells  autophagy  LC3
分类号:
S852.65
DOI:
10.3969/j.issn.1008-0425.2017.09.04
文献标志码:
A
摘要:
为研究马传染性贫血病毒(EIAV)感染对马胎皮细胞(FED)自噬的影响,本研究将EIAV感染FED以及过表达GFP-LC3的FED细胞,进行自噬现象的检测。结果显示,通过电镜观察到EIAV感染组细胞出现典型的双层膜结构的自噬小体,western blot检测到EIAV感染组细胞的LC3-II的表达量显著升高,通过激光共聚焦显微镜观察到EIAV感染的过表达GFP-LC3的FED细胞有明显的绿色荧光聚集。上述结果表明EIAV感染FED细胞后可以显著诱导FED细胞发生自噬。该研究结果为进一步研究自噬对EIAV复制和感染的调控奠定基础。
  
Abstract:
To investigate whether Equine infectious anemia virus  (EIAV) infection trigger autophagy in the fetal equine dermal (FED) cell, the results demonstrated that EIAV infection was able to cause the accumulation of autophagosomes, which were identified by the accumulation of green fluorescent protein (GFP-LC3) dots, and the conversion of LC3-I to LC3-II. In addition, the increased double membrane vesicles in the infected cells were also observed compared with mock cells under electron microscope. Inconclusion, the data of our study provided evidence that EIAV infection could induce the autophagic process in FED cells, which established the basis for further studies on effects of autophagy on EIAV replication and infection.
  

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(本文编辑:彭永刚)

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更新日期/Last Update: 2017-09-19