[1]冷依伊,任梅渗,蒙正群,等.5种猪病毒性传染病病原的多重PCR检测方法的建立[J].中国预防兽医学报,2017,(02):123-126.[doi:10.3969/j.issn.1008-0425.2017.02.10]
 LENG Yi-yi,REN Mei-shen,MENG Zheng-qun,et al.Development of multiplex PCR for detection of 5 pathogenic viruses to pigs[J].Chinese journal of preventive veterinary medicine,2017,(02):123-126.[doi:10.3969/j.issn.1008-0425.2017.02.10]
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5种猪病毒性传染病病原的多重PCR检测方法的建立()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2017年02
页码:
123-126
栏目:
诊断和检测技术
出版日期:
2017-02-15

文章信息/Info

Title:
Development of multiplex PCR for detection of 5 pathogenic viruses to pigs
文章编号:
1008-0589(2017)02-0123-04
作者:
 

冷依伊12任梅渗12蒙正群12张鹏飞12杨泽晓1姚学萍1王 印12*

 (1. 四川农业大学 动物医学院,四川 成都 611130;2. 动物疫病与人类健康四川省重点实验室,四川 成都 611130)
Author(s):
 

LENG Yi-yi12 REN Mei-shen12 MENG Zheng-qun12 ZHANG Peng-fei12 YANG Ze-xiao1 YAO Xue-ping2 WANG Yin12*

 (1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China)
关键词:
:多重PCR非洲猪瘟病毒猪口蹄疫病毒猪水疱性口炎病毒猪瘟病毒猪伪狂犬病病毒
Keywords:
: multiplex PCR  ASFV  VSV  FMDV  CSFV  PRV
分类号:
S852.65
DOI:
10.3969/j.issn.1008-0425.2017.02.10
文献标志码:
A
摘要:
为建立同时检测非洲猪瘟病毒(ASFV)、水疱性口炎病毒(VSV)、猪口蹄疫病毒(FMDV),猪瘟病毒(CSFV)以及猪伪狂犬病病毒(PRV)的多重RT-PCR检测方法,本研究根据GenBank中登录的参考病毒株序列,选择各病毒的保守序列设计5对特异性引物,通过优化反应条件,建立了一种可以同时快速检测以上5种病毒的多重RT-PCR 检测方法。结果显示,该方法对不同的细菌或病毒模板扩增结果均为阴性,特异性强;敏感性试验表明该方法对PRV、CSFV、ASFV、VSV和FMDV的核酸最少检出量分别为8.82×103拷贝/μL、6.87×104拷贝/μL、5.71拷贝/μL、4.93×104拷贝/μL和4.32×102拷贝/μL。以上结果表明该方法快速、灵敏、特异性强,对以上5种猪病病毒能够进行快速鉴别检测,为其临床诊断与流行病学调查提供了有效的检测方法。
  
Abstract:
In order to establish an rapid method to detect Africa swine fever virus (ASFV), vesicular stomatitis virus (VSV), foot and mouth disease virus (FMDV), classical swine fever virus (CSFV) and porcine pseudorabies virus (PRV). In this study, the method of multiplex PCR for detection of these 5 diseases was developed with the primers designed and synthesized according to the conservative sequences in reference strains of each kind of the virus. Through the optimization of reaction conditions, the method was specific for the 5 virus assay, but no any amplification was found for related pathogenic viruses. The detection limit of this method was  8.82×103, 6.87×104, 5.71, 4.93×104 and 4.32×102 copies/μL for PRV, CSFV, ASFV, VSV and FMDV, respectively. These data indicated that the rapid, sensitive and specific detection method could be applied in these 5 virus detections for clinical diagnosis and pathogenic epidemiologic investigation.
  

参考文献/References:

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                                                  (本文编辑:彭永刚)

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更新日期/Last Update: 2017-03-12