[1]赵战勤,刘倩玉,席晓剑,等.猪源A型和D型多杀性巴氏杆菌强毒菌株的生物学特性比较研究[J].中国预防兽医学报,2016,(05):366-370.[doi:10.3969/j.issn.1008-0589.2016.05.06]
 ZHAO Zhan-qin,LIU Qian-yu,XI Xiao-jian,et al.A comparative study on biological characterization of Pasteurella multocida serogroups A and D isolates from swine in China[J].Chinese journal of preventive veterinary medicine,2016,(05):366-370.[doi:10.3969/j.issn.1008-0589.2016.05.06]
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猪源A型和D型多杀性巴氏杆菌强毒菌株的
生物学特性比较研究
分享到:

《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2016年05
页码:
366-370
栏目:
病原生物学
出版日期:
2016-05-15

文章信息/Info

Title:
A comparative study on biological characterization of Pasteurella
multocida serogroups A and D isolates from swine in China
文章编号:
1008-0589(2016)05-0366-05
作者:
 赵战勤1刘倩玉1席晓剑1王 乐1邓 雯1*薛 云2张春杰1
(1. 河南科技大学 动物科技学院/河南省高等学校环境与畜产品安全重点学科开放实验室,河南 洛阳 471003;
2. 河南科技大学 医学技术与工程学院/微生物检验实验室,河南 洛阳 471003)
Author(s):
 ZHAO Zhan-qin1 LIU Qian-yu1 XI Xiao-jian1 WANG Le1 DENG Wen1* XUE Yun2 ZHANG Chun-jie1
(1. Key-Disciplines Lab of Safety of Environment and Animal Product, College of Animal Science and Technology,
Henan University of Science and Technology, Luoyang 471003, China; 2. Lab of Microbiology, College of Medical Technology
and Engineering, Henan University of Science and Technology, Luoyang 471003, China)
关键词:
 多杀性巴氏杆菌血清型生物学特性
Keywords:
 swine Pasteurella multocida serotypes biological characteristics
分类号:
S852.61
DOI:
10.3969/j.issn.1008-0589.2016.05.06
文献标志码:
A
摘要:
 为分析猪源A型和D型多杀性巴氏杆菌(PM)的生物学特性差异,本研究对5株A型和5株D型猪源PM强毒菌株的生化特征、生长特性、毒力特性、16S rRNA基因分子特征等生物学特性进行了比较研究。在TSB液体培养基中的生长曲线测定结果表明这些菌株均能够高密度发酵培养;21种生化试验的反应结果表明,10株PM的生化反应均不一致,即使同一血清型的不同菌株之间也具有较大差别。对小鼠毒力试验结果表明,A型PM菌株的毒力(LD50为4 cfu~26 cfu)显著强于D型菌株(LD50为2.1×104 cfu~5.2×105 cfu)。16S rRNA基因的序列分析结果表明,16S rRNA基因非常保守,这10株PM之间的同源性均在99.8 %以上;在1 505 bp序列中,仅存在第57 bp (T/C)、239 bp (T/C)和376 bp (G/C)处3个多态性位点,并且不具有型特异性。本研究为猪源PM灭活疫苗菌株的筛选等提供了实验依据。
Abstract:
 Serogroups A and D isolates of the Pasteurella multocida are the dominant causes of swine pasteurellosis in China. The biological characteristics of 10 field isolates of P.multocida, such as the growth rate, carbohydrate utilization, and virulence in mice were tested and the 16S rRNA genes of 5 serogroup A and 5 serogroup D of P.multocida isolates were also determined in this study. Replications of the isolates in tryptic soy broth containing 5% bovine serum showed that the highest colony-forming units (cfu) for serogroups A and D of P.multocida were 2.4×109 to 4.3×109 cfu/mL and 2.8×109 to 5.5×109 cfu/mL, respectively. There were different fermentation patterns between the serogroups A and D isolates for 21 different carbohydrates, and the difference existed even among the isolates in the same serogroup P.multocida. The serogroup A isolates showed more virulent in the BALB/c mice with the LD50 ranging from 4 to 26 cfu, compared to the serogroup A isolates with the LD50 ranging from 2.1×104 to 5.2×105 cfu, and no significant difference was found among the isolates within the same serogroup. The 16S rRNA gene sequencing and phylogenetic analysis demonstrated that the 16S rRNA genes of the 10 field isolates were relatively conservative.However, three single nucleotide polymorphisms (SNPs) were found in the 16S rRNA genes (1,505 bp) of these 10 P.multocida isolates, which located at 57 bp (T/C), 239 bp (T/C), and 376 bp (G/C), respectively. In conclusion, this study provides a basis for the further study on the pathogenesis and vaccine development of P.multocida.

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备注/Memo

备注/Memo:
收稿日期:2015-09-02
基金项目:国家自然科学基金项目(31302106);河南科技大学重大产学研培育项目(2015ZDCXY04)
作者简介:赵战勤(1980-),男,河南兰考人,副教授,博士,主要从事家畜传染病及其疫苗研究.
*通信作者:E-mail:wen.dkj@163.com
更新日期/Last Update: 2016-06-07