[1]秦 蕾*,徐 静,张晓君.异育银鲫非O1/非O139群霍乱弧菌的分离及鉴定[J].中国预防兽医学报,2013,(02):130-133.[doi:doi: 10.3969/j.issn.1008-0589.2013.02.12]
 QIN Lei*,XU Jing,ZHANG Xiao-jun.Isolation and identification of non-O1/non-O139 Vibrio cholerae from gibel carp[J].Chinese journal of preventive veterinary medicine,2013,(02):130-133.[doi:doi: 10.3969/j.issn.1008-0589.2013.02.12]
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异育银鲫非O1/非O139群霍乱弧菌的分离及鉴定
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2013年02期
页码:
130-133
栏目:
病原生物学
出版日期:
2013-02-15

文章信息/Info

Title:
Isolation and identification of non-O1/non-O139 Vibrio cholerae from gibel carp
文章编号:
1008-0589(2013)02-0130-04
作者:
秦 蕾*徐 静张晓君
(淮海工学院 海洋学院,江苏 连云港 222005)
Author(s):
QIN Lei* XU Jing ZHANG Xiao-jun
(College of Marine Science, Huaihai Institute of Technology, Lianyungang 222005, China)
关键词:
非O1/非O139群霍乱弧菌异育银鲫表型特征16S rDNA药敏试验
Keywords:
non-O1/non-O139 Vibrio cholerae gibel carp phenotypic characteristics 16S rDNA antibiotic susceptibility testing
分类号:
S852.61
DOI:
doi: 10.3969/j.issn.1008-0589.2013.02.12
文献标志码:
A
摘要:
为鉴定引起异育银鲫发病的疫情的病原,本研究从病鱼体内分离到细菌分离株,经动物回归试验证明为导致异育银鲫发病的致病原。对病原菌株进行形态学、生理生化测定以及16S rDNA序列分析,显示该菌株与霍乱弧菌(Vibrio cholerae)表型特征一致,而且在系统发育树中也是与霍乱弧菌相聚类,两者16S rDNA序列相似性高达99.93 %。综合表型特征与基因序列分析的结果表明,该病原菌株应归属于霍乱弧菌。血清型鉴定为非O1/非O139群霍乱弧菌。药物敏感性试验结果显示该株霍乱弧菌对喹诺酮类、头孢类、氨基糖苷类、大环内酯类,四环素类以及β-内酰胺类等药物敏感。
Abstract:
An infectious disease occurred in a gibel carp farm in 2010. The dominant bacteria were isolated from diseased fish and proved to be causal agent of the disease by experimental infectionin gibel carp. Conventional physiological and biochemical tests showed that the isolates had phenotypic characteristics in according with reference strain of Vibrio cholerae. And 16S rDNA sequence was analyzed revealing that it was most closely related to V.cholerae with 99.93% similarity. Therefore, the isolates were finally identified as V.cholerae. Antibiotic susceptibility testing showed that the isolates were sensitive to quinolones, cephems, aminoglycosides, macrolide, tetracyclines and β-lactams.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-12-30
基金项目:江苏省海洋生物技术重点建设实验室开放课题(2008HS018);淮海工学院博士科研启动基金项目(KQ07051)
作者简介:秦 蕾(1978-),女,山东烟台人,副教授,博士,主要从事水产动物疾病的防治研究.
*通信作者:E-mail:qinlei1978@163.com
更新日期/Last Update: 2013-01-25