[1]纪晓琳,王 琦,高玉龙,等.J亚群禽白血病病毒分离株HLJ09SH01株感染性克隆的 构建及其致病性研究[J].中国预防兽医学报,2013,(01):15.
 JI Xiao-lin,WANG Qi,GAO Yu-long,et al.Construction of an infectious molecular clone of a subgroup J avian leukosis virus isolation HLJ09SH01 and its pathogenicity[J].Chinese journal of preventive veterinary medicine,2013,(01):15.
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J亚群禽白血病病毒分离株HLJ09SH01株感染性克隆的 构建及其致病性研究
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2013年01期
页码:
15
栏目:
病原生物学
出版日期:
2013-01-15

文章信息/Info

Title:
Construction of an infectious molecular clone of a subgroup J avian leukosis virus isolation HLJ09SH01 and its pathogenicity
作者:
纪晓琳王 琦高玉龙王永强秦立廷祁小乐高宏雷王笑梅*
(中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/禽传染病研究室,黑龙江 哈尔滨 150001)
Author(s):
JI Xiao-lin WANG Qi GAO Yu-long WANG Yong-qiang QIN Li-ting QI Xiao-le GAO Hong-lei WANG Xiao-mei*
(Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China)
关键词:
J亚群禽白血病病毒感染性分子克隆致病性
Keywords:
avian leukosis virus subgroup J infectious molecular clone pathogenicity
摘要:
为构建J亚群禽白血病病毒(ALV-J)分离株的感染性克隆并鉴定其对蛋鸡和肉鸡致病力的差异,本研究从黑龙江地区分离鉴定一株ALV-J 分离株HLJ09SH01,以其核酸为模板,采用 PCR方法分3段扩增其前病毒cDNA,PCR产物经克隆酶切后依次连接,获得一个含有完整ALV-J 前病毒 cDNA的重组质粒,命名为pBlue-HLJ09SH01。将其转染DF-1细胞,进行病毒拯救。通过间接免疫荧光、禽白血病抗原试剂盒检测及反转录酶活性试剂盒检验,表明拯救的病毒为ALV-J,命名为rHLJ09SH01。将其分别人工接种11日龄蛋鸡和肉鸡鸡胚,孵育出壳后隔离饲养32周。致瘤性试验结果表明,rHLJ09SH01可以导致57.9 %的蛋鸡发生肿瘤,83.3 %的肉鸡发生肿瘤。表明该感染性克隆具有亲本病毒的致病性。
Abstract:
To construct the infectious clone of subgroup J avian leukosis virus (ALV-J) and identification of the oncogenicity of the rescued virus in layers and broilers, the sequence the provirus cDNA of ALV-J was amplified by PCR from ALV-J HLJ09SH01 isolated in Heilongjiang province and the infectious clone of pBlue-HLJ09SH01 was constructed based on the vector of pBluescript II KS(+). The rescued virus of rHLJ09SH01 was generated by transfecting pBlue-HLJ09SH01 into the DF-1 cells and identified by indirect immunofluorescence assay, avian leukosis virus antigen test kit, reverse transcriptase assay. Furthermore, the 11 day old embryos of SPF layers and commercial broilers were inoculated with the rHLJ09SH01 via allantoic cavity and the results showed the rHLJ09SH01 caused typical tumors in SPF layer (57.9%) and broiler (83.3%) during 32 week period of observation after hatching, which demonstrated that the rescued virus possesses pathogenicity as parent virus.

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更新日期/Last Update: 2013-01-20