[1]唐 续#,杨焕良#,鄢明华,等.一株人源H1N1亚型猪流感病毒的进化分析与生物学特性研究[J].中国预防兽医学报,2012,(04):270-273.[doi:10.3969/j.issn.1008-0589.2012.04.05]
 TANG Xu *,YANG Huan-liang *,YAN Ming-hua,et al.Evolution analysis and biological characteristics of a human origin H1N1 subtype swine influenza virus[J].Chinese journal of preventive veterinary medicine,2012,(04):270-273.[doi:10.3969/j.issn.1008-0589.2012.04.05]
点击复制

一株人源H1N1亚型猪流感病毒的进化分析与生物学特性研究
分享到:

《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2012年04期
页码:
270-273
栏目:
病原生物学
出版日期:
2012-04-15

文章信息/Info

Title:
Evolution analysis and biological characteristics of a human origin H1N1 subtype swine influenza virus
文章编号:
1008-0589(2012)04-0270-04
作者:
唐 续1# 杨焕良1# 鄢明华2李秀丽2陈 艳1孟沙沙1尹 航1辛晓光1步志高1乔传玲1* 陈化兰1*
1. 中国农业科学院哈尔滨兽医研究所 农业部动物流感重点开放实验室/兽医生物国家重点开放实验室,黑龙江 哈尔滨 150001; 2. 天津市畜牧兽医研究所,天津 300112
Author(s):
TANG Xu 1* YANG Huan-liang 1* YAN Ming-hua 2 LI Xiu-li 2 CHEN Yan 1 MENG Sha-sha 1 YI Hang 1 XIN Xiao-guang 1 BU Zhi-gao 1 QIAO Chuan-ling 1* CHEN Hua-lan 1*
1. Animal Influenza Key Laboratory of the Ministry of Agricultural, State Key Laboratory of Veterinary Biotechnology,
Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;
2. Tianjin Institute of Animal Husbandry and Veterinary Science, Tianjin 300112, China
关键词:
猪流感病毒人源H1N1亚型遗传演化分析抗原性分析致病性
Keywords:
swine influenza human origin H1N1 genetic analysis antigenic analysis pathogenicity
分类号:
S852.65
DOI:
10.3969/j.issn.1008-0589.2012.04.05
文献标志码:
A
摘要:
为了解猪流感病毒(SIV)的变异情况,我们2009年11月从河北某养殖场采集呈流感症状的猪鼻拭子40份,接种10日龄SPF鸡胚,分离到一株猪流感病毒,通过RT-PCR和血凝抑制试验鉴定为H1N1亚型,命名为A/swine/Hebei/15/2009(H1N1),其全基因序列测定及同源性分析发现,8个基因片段均与2000年左右H1N1人流感病毒有较高的同源性。系统遗传演化显示,该病毒分离株是由2000年人源H1N1流感病毒A/Dunedin/ 2/2000(H1N1) 进化而来。抗原性分析显示该株与甲型H1N1流感病毒和经典H1N1病毒株抗原性差异较大。对小鼠致病性试验表明该病毒株可以直接感染小鼠并导致小鼠轻微临床症状和组织病理学变化,但不致死小鼠,表现为低致病性。
Abstract:
To isolate the swine influenza virus (SIV), a total of 40 nasal swabs samples were collected from pigs which had influenza like symptoms in Hebei province in November, 2009. A virus was isolated by inoculation of 10-day-old embryonated eggs via allantoic cavities and identified as H1N1 subtype by hemagglutination inhibition (HI) test and RT-PCR, designated A/Swine/Hebei/15/2009(H1N1). The complete genes were sequenced and the results shown that the eight gene segments of the isolate were closely identical to human H1N1 influenza virus A/Dunedin/2/2000(H1N1). The genetic analysis indicated that the virus was directly derived from the human H1N1 influenza virus. HI tests found that the isolate had less cross reaction with the reference strains of pandemic H1N1 or classical swine H1N1. In addition, the pathogenic tests in mice demonstrated the virus was able to infect the mice directly and cause less clinical sings and histopathologic change, but no mortality occurred.

参考文献/References:

[1]Sun Lei, Zhang Gui-hong, Shu Yue-long, et al. Genetic correlation between H3N2 human and swine influenza viruses [J]. J Clin Virol, 2009, 44(2): 141-144.
[2]Yu Hai, Zhou Yan-jun, Li Guo-xin, et al. Further evidence for infection of pigs with human-like H1N1 influenza viruses in China [J]. Virus Res, 2009, 140(1-2): 85-90.
[3]Qi Xian, Lu Cheng-ping. Genetic characterization of novel reassortant H1N2 influenza A viruses isolated from pigs in southeastern China [J]. Arch Virol, 2006, 151(11): 2289-2299.
[4]Gamblin S J, Haire L F, Russell R J, et al. The structure and receptor binding properties of the 1918 influenza hemagglutinin[J]. Science, 2004, 303(5665): 1838-1842.
[5]Igarashi M, Ito K, Kida H et al. Genetically destined potential for N-linked glycosylation of influenza virus hemagglutinin [J].Virology, 2008, 376: 323-329.
[6]Matrosovich M, Zhou Nan-nan, Kawaoka Y, et al. The surface glycoproteins of H5 influenza viruses isolated from humans, chickens, and wild aquatic birds have distinguishable properties [J]. Virology, 1999, 73(2): 1146-1155.
[7]Subbarao E K, London W, Murphy B R. A single amino acid in the PB2 gene of influenza A virus is a determinant of host range [J]. J Virol, 1993, 67(4): 1761-1764.
[8]Li Ze-jun, Chen Hua-lan, Jiao Pei-rong, et al. Molecular basis of replication of duck H5N1 influenza viruses in a mammalian mouse model [J]. J Virol, 2005, 79(18): 12058-12064.
[9]Yu Hai, Hua Rong-hong, Zhang Qiang, et al. Genetic evolution of swine influenza A (H3N2) viruses in China from 1970 to 2006 [J]. J Clin Microbiol, 2008, 46(3): 1067-1075.
[10]Brown I H. The epidemiology and evolution of influenza viruses in pigs [J]. Vet Microbiol, 2000, 74(1-2): 29-46.

相似文献/References:

[1]郭利敏,乔传玲*,陈 艳,等.利用真核表达的猪流感病毒NP蛋白制备其特异性的单克隆抗体[J].中国预防兽医学报,2010,(06):460.
 GUO Li-min,QIAO Chuan-ling*,CHEN Yan,et al.Preparation of the monoclonal antibodies using swine influenza virus NP protein expressed by eukaryotic expression vector[J].Chinese journal of preventive veterinary medicine,2010,(04):460.
[2]罗 俊,刘业兵*,陈 坚,等.双重RT-PCR方法检测猪流感病毒和猪繁殖与呼吸综合征病毒[J].中国预防兽医学报,2010,(09):687.
 LUO Jun,LIU Ye-bing*,CHEN Jian,et al.Establishment of a duplex RT-PCR for the detection of swine influenza virus and porcine reproductive and respiratory syndrome virus[J].Chinese journal of preventive veterinary medicine,2010,(04):687.
[3]张鹏超,师小潇,哈 卓,等.猪流感病毒SYBR GreenⅠ定量RT-PCR检测方法的建立及应用[J].中国预防兽医学报,2010,(10):768.[doi:10.3969/j.issn.1008-0589.2010.10.06]
 ZHANG Peng-chao,SHI Xiao-xiao,HA Zhuo,et al.Establishment of SYBR GreenⅠreal time RT-PCR for detecting swine influenza virus[J].Chinese journal of preventive veterinary medicine,2010,(04):768.[doi:10.3969/j.issn.1008-0589.2010.10.06]
[4]李国新,郑宝亮,金媛媛,等.分子佐剂C3d增强猪流感病毒HA-DNA疫苗免疫原性的研究[J].中国预防兽医学报,2008,(10):814.
 LI Guo-xin,ZHENG Bao-liang,JIN Yuan-yuan,et al.C3d-induced immune enhancement of the swine influenza HA expressed by a DNA vaccine[J].Chinese journal of preventive veterinary medicine,2008,(04):814.
[5]韩庆功,乔传玲*,杨焕良,等.猪源H9N2亚型流感病毒HA基因的序列测定及真核表达[J].中国预防兽医学报,2007,(12):916.
 HAN Qing-gong,QIAO Chuan-ling*,YANG Huan-liang,et al.Sequencing and eukaryotic expression of HA gene of H9N2 subtype influenza virus from swine[J].Chinese journal of preventive veterinary medicine,2007,(04):916.
[6]陈 艳,辛晓光,杨焕良,等.猪流感抗体间接ELISA检测方法的建立[J].中国预防兽医学报,2007,(04):308.
 CHEN Yan,XIN Xiao-guang,YANG Huan-liang,et al.Establishment of an indirect ELISA for detection of antibodies against swine influenza virus[J].Chinese journal of preventive veterinary medicine,2007,(04):308.
[7]郑宝亮,田志军,李 若,等.表达H3N2亚型猪流感病毒HA的重组伪狂犬病病毒对仔猪的免疫效力评价[J].中国预防兽医学报,2007,(02):115.
 ZHENG Bao-liang,TIAN Zhi-jun,LI Ruo,et al.Protection of piglets from virulent challenge after immunization with a recombinant pseudorabies virus expressing the HA gene of a H3N2 subtype swine influenza virus[J].Chinese journal of preventive veterinary medicine,2007,(04):115.
[8]张瑞华,崔红玉,徐明举,等.H9N2亚型猪流感病毒对小鼠的致病性及其分子特性研究[J].中国预防兽医学报,2011,(07):512.
 ZHANG Rui-hua,CUI Hong-yu,XU Ming-ju,et al.Molecular characterization and pathogenicity of swine influenza H9N2 subtype virus in mice[J].Chinese journal of preventive veterinary medicine,2011,(04):512.
[9]尹 航,杨焕良,陈 艳,等.甲型H1N1流感病毒实时荧光定量PCR检测方法的建立[J].中国预防兽医学报,2013,(01):36.
 YIN Hang,YANG Huan-liang,CHEN Yan,et al.Establishment of one-step TaqMan real time RT-PCR for Pandemic/2009 influenza viruses in swine[J].Chinese journal of preventive veterinary medicine,2013,(04):36.
[10]孟沙沙,乔传玲*,陈 艳,等.一株类禽型H1N1亚型猪流感病毒的反向遗传系统的建立[J].中国预防兽医学报,2013,(02):91.[doi:doi: 10.3969/j.issn.1008-0589.2013.02.02]
 MENG Sha-sha,QIAO Chuan-ling*,CHEN Yan,et al.Establishment of reverse genetic system for an avian-like swine H1N1 influenza virus[J].Chinese journal of preventive veterinary medicine,2013,(04):91.[doi:doi: 10.3969/j.issn.1008-0589.2013.02.02]

备注/Memo

备注/Memo:
收稿日期:2012-02-06
基金项目:国家973计划项目(2010CB534001):哈尔滨市科技攻关计划(2009AA6BN078);天津市科技支撑计划重大项目(10ZCZDNC02800)
共同第一作者:唐 续(1984-),男,山西朔州人,硕士研究生,主要从事动物流感研究;
杨焕良(1975-),男,辽宁丹东人,助理研究员,博士研究生,主要从事动物流感研究.
*通信作者:E-mail:qcl@hvri.ac.cnhlchen1@yahoo.com
更新日期/Last Update: 2012-04-18