[1]刘孝珍,陈建飞,时洪艳,等.2011年猪流行性腹泻病毒的遗传变异分析[J].中国预防兽医学报,2012,(03):180-183.[doi:10.3969/j.issn.1008-0589.2012.03.04]
 LIU Xiao-zhen,CHEN Jian-fei,SHI Hong-yan,et al.Genetic variation analysis of porcine epidemic diarrhea virus isolated in 2011[J].Chinese journal of preventive veterinary medicine,2012,(03):180-183.[doi:10.3969/j.issn.1008-0589.2012.03.04]
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2011年猪流行性腹泻病毒的遗传变异分析
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2012年03期
页码:
180-183
栏目:
病原生物学
出版日期:
2012-03-15

文章信息/Info

Title:
Genetic variation analysis of porcine epidemic diarrhea virus isolated in 2011
文章编号:
1008-0589(2012)03-0180-04
作者:
刘孝珍12陈建飞2时洪艳2张 鑫2石 达2刘胜旺12冯 力12*
1. 东北农业大学 生命科学学院,黑龙江 哈尔滨 150030;2. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/猪传染病研究室,黑龙江 哈尔滨 150001
Author(s):
LIU Xiao-zhen12 CHEN Jian-fei2 SHI Hong-yan2 ZHANG Xin2 SHI Da2 LIU Sheng-wang12 FENG Li12*
1. College of Life Science, Northeast Agricultural University, Harbin 150030, China; 2. Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
关键词:
猪流行性腹泻病毒遗传变异S1基因
Keywords:
porcine epidemic diarrhea virus genetic variation S1 gene
分类号:
S852.65
DOI:
10.3969/j.issn.1008-0589.2012.03.04
文献标志码:
A
摘要:
为分析猪流行性腹泻病毒(PEDV)的遗传变异情况,本研究设计合成2对扩增S1基因的引物,利用套式RT-PCR方法,对2011年分离的17个PEDV S1基因进行扩增、克隆和序列测定;并将其进行比对和遗传演化分析。结果表明:17个PEDV S1基因序列与参考病毒株S1基因核苷酸序列同源性为90.54 %~99.96 %,与经典病毒株CV777相比,其中有6个S1基因在453 bp~454 bp处存在3个核苷酸的缺失;一个在453 bp~454 bp处缺失6个核苷酸;其它10个S1基因在173 bp~186 bp之间存在12个核苷酸的插入,413 bp~417 bp之间有3个核苷酸的插入,467 bp~468 bp处缺失6个核苷酸,插入和缺失位点与韩国病毒株KNU-0901、KNU-0905、KNU-0801相似。S1基因系统进化树分析表明,PEDV S1基因分为3群,其中7个S1基因属于PEDVⅠ群,另外10个属于PEDV Ⅲ群。
Abstract:
Porcine epidemic diarrhea virus (PEDV) causes an acute and highly contagious enteric disease in swine. To investigate the variation in S1 gene of PEDV, a total of 17 PEDV S1 genes were cloned and sequenced from different swine breeding farms in different provinces in 2011. Compared with CV777, there were 3 nucleotides deletion between 453 bp to 454 bp in 6 isolates; There were 6 nucleotide deletion between 453 bp to 454 bp in one isolate. There were 12 nucleotide insertions between173 bp to 186 bp, 3 nucleotide insertions between 413 bp to 417 bp and 6 nucleotide deletion between 467 bp to 468 bp, respectively, in the other 10 isolates. The nucleotide insertion and deletion were similar to KNU-0901, KNU-0905, KNU-0801 strain isolated in Korea. Sequence analysis showed that S1 gene shared 90.54% to 99.96% nucleotide identities with PEDV reference sequences. Phylogenetic analysis revealed that 7 of PEDV S1 gene belong to the first group. Other 10 of PEDV S1 gene belong to the third group.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-10-13
基金项目:哈尔滨市科技攻关项目(2009AA6AN029)
作者简介:刘孝珍(1984-),女,内蒙古乌兰察布人,硕士研究生,主要从事实验动物研究.
*通信作者:E-mail:fl@hvri.ac.cn
更新日期/Last Update: 2013-05-14