[1]秦永丽,孙恩成,耿宏伟,等.抗蓝舌病病毒VP7和NS2蛋白单克隆抗体的制备及鉴定[J].中国预防兽医学报,2011,(10):816-819.[doi:10.3969/j.issn.1008-0589.2011.10.15]
 QIN Yong-li,SUN En-cheng,GENG Hong-wei,et al.Production and identification of monoclonal antibodies against VP7 and NS2 of bluetongue virus[J].Chinese journal of preventive veterinary medicine,2011,(10):816-819.[doi:10.3969/j.issn.1008-0589.2011.10.15]
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抗蓝舌病病毒VP7和NS2蛋白单克隆抗体的制备及鉴定
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2011年10期
页码:
816-819
栏目:
免疫学
出版日期:
2011-10-15

文章信息/Info

Title:
Production and identification of monoclonal antibodies against VP7 and NS2 of bluetongue virus
文章编号:
1008-0589(2011)10-0816-04
作者:
秦永丽1 孙恩成1耿宏伟12杨 涛1刘霓红1赵 晶1王凌凤1徐青元1蔡绪禹1李文京3吴东来1*
1. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/农业部兽医公共卫生重点开放实验室,
黑龙江 哈尔滨150001;2. 东北农业大学 动物医学学院,黑龙江 哈尔滨150030;3. 中国动物疫病预防控制中心,北京 100125
Author(s):
QIN Yong-li 1 SUN En-cheng 1 GENG Hong-wei 12 YANG Tao 1 LIU Ni-hong 1 ZHAO Jing 1 WANG Ling-feng 1 XU Qing-yuan 1 CAI Xu-yu 1 LI Wen-jing 3 WU Dong-lai 1*
1. Key Laboratory of Veterinary Public Health, Ministry of Agricultural, State Key Laboratory of Veterinary Biotechnology,
Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China; 2. College of Animal Medicine, Northeast Agricultural University, Harbin 150030, China; 3. China Animal Disease Control Center, Beijing 100125, China
关键词:
蓝舌病病毒VP7 蛋白NS2蛋白单克隆抗体
Keywords:
bluetongue virus VP7 NS2 monoclonal antibody
分类号:
S852.65
DOI:
10.3969/j.issn.1008-0589.2011.10.15
文献标志码:
A
摘要:
为建立蓝舌病病毒(BTV)的检测方法和研究该病毒蛋白的功能,本研究利用BTV血清8型(BTV8)免疫BALB/c小鼠,取免疫后的小鼠脾淋巴细胞与SP2/0细胞融合,制备单克隆抗体(MAb)。并以BTV8作为包被抗原建立间接ELISA方法,经筛选获得了8株稳定分泌抗BTV8 MAb的杂交瘤细胞株(1B2、1F6、2B1、2D10、3B6、3D9、4D4和4D12)。Western blot结果显示,MAb 1F6、2B1、2D10、3B6、3D9 与 BTV8 VP7 蛋白反应,MAb B2、4D4、4D12 与 BTV8 NS2蛋白反应。间接免疫荧光结果显示,该8株MAb与24型BTV血清型呈不同的反应谱系。本研究所获得的MAb为建立BTV免疫学检测方法和相关病毒蛋白的功能研究提供了实验依据。
Abstract:
To prepare monoclone antibody (MAb) against bluetongue virus (BTV), SP2/0 myeloma cells were fused with spleen cells of BALB/c mice immunized with BTV serotype 8, and screened by indirect ELISA coated with BTV8. Eight MAbs (1B2, 1F6, 2B1, 2D10, 3B6, 3D9, 4D4 and 4D12) against BTV were produced. The result of western blot indicated that the MAbs of 1F6, 2B1, 2D10, 3B6, 3D9 reacted to VP7 protein, and the rest of MAbs reacted to NS2 protein. The results of IFA showed that all the MAbs had different reactivity with BTV1-24 serotypes. The MAbs produced in this study are useful tools for developing immunological diagnostic techniques of BTV and investigating proteins related to BTV.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-06-12
基金项目:国家863项目(2011AA10A212);黑龙江省自然科学基因项目(ZJN-0602-01)
作者简介:秦永丽(1984-),女,山东青岛人,硕士研究生,主要从事分子病毒学和分子免疫学研究.
*通信作者:E-mail:dlwu@hvri.ac.cn
更新日期/Last Update: 2012-03-29