[1]王琦,王永强,康忠惠,等.蛋鸡J亚群禽白血病病毒SD1009分离株的分离鉴定及全基因组序列分析[J].中国预防兽医学报,2011,(08):593-597.
 WANG Qi,WANG Yong-qiang,KANG Zhong-hui,et al.Isolation and identification molecular characterization of a subgroup J avian leukosis virus isolate SD1009[J].Chinese journal of preventive veterinary medicine,2011,(08):593-597.
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蛋鸡J亚群禽白血病病毒SD1009分离株的分离鉴定及全基因组序列分析
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2011年08期
页码:
593-597
栏目:
病原生物学
出版日期:
2011-08-15

文章信息/Info

Title:
Isolation and identification molecular characterization of a subgroup J avian leukosis virus isolate SD1009
作者:
王琦;王永强;康忠惠;高玉龙;潘伟;李久宽;秦立廷;祁小乐;高宏雷;王笑梅;
中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室;
Author(s):
WANG QiWANG Yong-qiangKANG Zhong-huiGAO Yu-longPAN WeiLI Jiu-kuan QIN Li-tingQI Xiao-leGAO Hong-leiWANG Xiao-mei*
Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Harbin 150001,China
关键词:
J亚群禽白血病病毒蛋鸡分离鉴定全基因组序列分析
Keywords:
avian leukosis virus subgroup J layer chicken isolation molecular characterization
摘要:
为分析我国J亚群禽白血病病毒(ALV-J)蛋鸡分离株的进化关系,本研究将山东省某鸡场采集的蛋鸡病料样品接种DF-1细胞系,利用ELISA群特异性抗原检测以及亚群特异性间接免疫荧光方法,分离鉴定得到一株ALV-J,命名为SD1009,并对其进行全基因组测序,将该序列与其他ALV-J代表性病毒株序列进行比较。结果表明:SD1009分离株的gag和pol基因相对保守,与各参考病毒株的同源性为95%~99%,env基因的同源性为91%~95%;在其5'UTR中出现了连续19 bp的插入突变,与TW-3577、SDAU09C3、JS09GY6、JS09GY3蛋鸡分离株的5'UTR基本一致,提示19 bp的插入现象可能是近年来蛋鸡ALV-J的进化趋势;此外,其3'UTR的rTM和DR区出现部分缺失现象,该缺失部分也可能与ALV-J进化相关。
Abstract:
A isolate of subgroup J avian leukosis virus(ALV-J),designed SD1009,was isolated from layer chicken in Shandong province and identified by PCR,ELISA and indirect immunofluorescence assay(IFA).The viral genome of this isolate was sequenced and compared with reference ALV-J strains in GenBank.The results show that the gag and pol of the isolate were relatively conservative,the sequence identity was ranged from 95% to 99% for gag and pol genes,and from 91% to 95% for env gene comparing with reference strains.However,a 19 bp insertion mutation in 5’ UTR of SD1009 was similar to TW-3577,SDAU09C3,JS09GY6 and JS09GY3 strains from layer chicken.Besides,partial deletion of the DR and rTM region in 3’UTR of SD1009 isolate was also identified in some recent isolated ALV-J.In conclution,those mutations might suggest evolution trend of ALV-J in layer chicken

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备注/Memo

备注/Memo:
基金:现代农业肉鸡产业技术体系建设(nycytx-42-G3-01);;国家自然科学基金(3107Z146)
更新日期/Last Update: 2011-09-29