[1]石 敏,田明星,王远萍,等.J亚群禽白血病病毒四川株SCMS1的分离及其囊膜基因序列分析[J].中国预防兽医学报,2011,(01):62-65.[doi:10.3969/j.issn.1008-0589.2011.01.15]
 SHI Min,TIAN Ming-xing,WANG Yuan-ping,et al.Isolation and sequence analysis of the env gene of subgroup J avian leukosis virus isolated from Sichuan area[J].Chinese journal of preventive veterinary medicine,2011,(01):62-65.[doi:10.3969/j.issn.1008-0589.2011.01.15]
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J亚群禽白血病病毒四川株SCMS1的分离及其囊膜基因序列分析
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2011年01期
页码:
62-65
栏目:
研究简报
出版日期:
2011-01-15

文章信息/Info

Title:
Isolation and sequence analysis of the env gene of subgroup J avian leukosis virus isolated from Sichuan area
文章编号:
1008-0589(2011)01-0062-04
作者:
石 敏1田明星1王远萍1邹年莉1林 艳1余 冲1赵芳芳1李 敏1黄 勇12*
1. 四川农业大学 动物医学院,四川 雅安 625014;
2. 四川农业大学 动物疫病与人类健康四川省重点实验室,四川 雅安 625014
Author(s):
SHI Min1 TIAN Ming-xing1 WANG Yuan-ping1 ZOU Nian-li1 LIN Yan1 YU Chong1 ZHAO Fang-fang1 LI Min1 HUANG Yong12*
1. College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China;
2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an 625014, China
关键词:
J亚群禽白血病病毒分离鉴定env基因序列分析
Keywords:
subgroup J avian leukosis virus isolation and identifation env gene subsequence analysis
分类号:
S852.65
DOI:
10.3969/j.issn.1008-0589.2011.01.15
文献标志码:
A
摘要:
为研究四川首例分离株SCMS1株J亚群禽白血病病毒(ALV-J)囊膜(env)基因是否产生新的变异,本实验通过将病毒接种鸡胚成纤维细胞、RT-PCR及PCR检测技术,对包括env基因和部分3’ UTR在内的片段进行PCR扩增、克隆和序列测定。结果发现该病毒gp85基因出现6个碱基的缺失,与HPRS-103原型株6995位~7242位对应的核酸序列也发生248 个碱基的缺失,包括整个rTM区域。这些碱基的缺失在病毒致病性变化中可能具有潜在的意义。
Abstract:
The env gene of subgroup J avian leukosis virus SCMS1 strain isolated from Sichuan area was amplified by PCR. Sequencing results indicated that there were 6 base pair deletion in gp85 gene. A 248 bp deletion in the nucleotides region corresponding to 6,995 bp to 7,242 bp of the HPRS-103 strain, including the entire domains of rTM. These mutations could play a meaningful role for virus pathogenicity variations.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2010-05-25
基金项目:教育部“长江学者和创新团队发展计划”创新团队项目(IRTO848)
作者简介:石 敏(1984-),女,河北唐山人,硕士研究生,主要从事家禽传染病的研究.
*通信作者:E-mail:hyong601@163.com
更新日期/Last Update: 2010-12-30