[1]王 新*,黄 山,周 婷,等.猪源耐甲氧西林金黄色葡萄球菌的耐药性及其SCCmec基因分型研究[J].中国预防兽医学报,2010,(12):975-977.[doi:10.3969/j.issn.1008-0589.2010.12.16]
 WANG Xin*,HUANG Shan,ZHOU Ting,et al.Antimicrobial susceptibility and SCCmec genetyping of methicillin-resistant Staphylococcus aureus isolates from swine[J].Chinese journal of preventive veterinary medicine,2010,(12):975-977.[doi:10.3969/j.issn.1008-0589.2010.12.16]
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猪源耐甲氧西林金黄色葡萄球菌的耐药性及其SCCmec基因分型研究
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2010年12期
页码:
975-977
栏目:
研究简报
出版日期:
2010-12-01

文章信息/Info

Title:
Antimicrobial susceptibility and SCCmec genetyping of methicillin-resistant Staphylococcus aureus isolates from swine
文章编号:
1008-0589(2010)12-0975-03
作者:
王 新1*黄 山2周 婷3刘俊宁1杨保伟1席美丽1申进玲1只 帅1孟江洪1
1. 西北农林科技大学 食品学院,陕西 杨凌 712100;
2. 四川省石棉县畜牧局,四川 石棉 625400;
3. 西北农林科技大学 动物医学院,陕西 杨凌 71210
Author(s):
WANG Xin1* HUANG Shan2 ZHOU Ting3 LIU Jun-ning1 YANG Bao-wei1 XI Mei-li1 SHEN Jin-ling1 ZHI Shuai1 MENG Jiang-hong1
1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;
2. Sichuan Shimian Husbandry Bureau, Shimian 625400, China;
3. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
关键词:
MRSA耐药性SCCmec分型
Keywords:
swine MRSA antimicrobial resistance SCCmec type
分类号:
S852.61
DOI:
10.3969/j.issn.1008-0589.2010.12.16
文献标志码:
A
摘要:
为了解陕西猪场耐甲氧西林金黄色葡萄球菌(MRSA)的耐药表型及其SCCmec基因型分布情况,我们于2009年1月~7月在陕西地区6个规模化猪场,采集鼻腔拭子共计173份,利用选择性培养基分离金黄色葡萄球菌(SA),采用多重PCR方法扩增其中是否含有nuc基因和mecA基因进行MRSA鉴定;以琼脂稀释法进行药敏试验;并通过多重PCR方法对SCCmec进行基因分型。结果显示,调查的6个猪场中有2个猪场分离到MRSA,共分离到83株SA,其中有14 株(16.9 %)为MRSA,均为SCCmec-IVb型;药敏结果显示MRSA除对CFP、CHL、AMK和VAN敏感外,对CIP、OXA耐药率分别为57.1 %和92.6 %,对FOX、TET、ERY、甲氧嘧啶和卡那霉素的耐药率均为100 %。结果显示了猪源SCCmec-IVb型MRSA株具有多重耐药特点,与人源SCCmec-IV型MRSA株耐药谱存在较大差异,这一结果提示猪源MRSA容易通过猪及其产品感染人。
Abstract:
To investigate antimicrobial resistance and molecular epidemiology profile of methicillin-resistant Staphylococcus aureus (MRSA) in Shanxi area, total of 173 swine nasal swabs samples were collected from 6 swine farms from Shanxi area and S.aureus were isolated using selective medium and confirmed by multiplex PCR. The MRSA isolates were identificated by duplex PCR with nuc gene and mecA gene, Staphylococcal chromosomal cassette (SCC) mec typing and antimicrobial susceptibility test. A total of 83 S. aureus were isolated and tested according to the growth characteristics on Baird-Parker plate and by multiplex PCR, and 14 (14/83) were identified positively for MRSA. These MRSA isolates were determined to be SCCmec type IVb and were multidrug-resistant strains, but susceptible to vancomycin, chloramphenicol, amikacin and cefoperazone, which was different from the SCCmec type IVb MRSA isolates from human. The results showed that prevalence of methicillin-resistant MRSA in pigs imposes a new threat to human health.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2010-05-09
基金项目:博士科研启动费(01140407)
作者简介:王 新(1973-),男,四川邛崃人,讲师,博士,主要从事食源性病原及分子生物学和食品安全研究.
*通信作者:E-mail:xinwang7516@yahoo.com.cn
更新日期/Last Update: 2010-12-13