[1]龚 倩,高淑琴,单晓枫,等.框镜鲤致病性维氏气单胞菌的分离鉴定[J].中国预防兽医学报,2010,(12):981-983.[doi:10.3969/j.issn.1008-0589.2010.12.18]
 GONG Qian,GAO Shu-qin,SHAN Xiao-feng,et al.Isolation and identification of pathogenic Aeromonas veronii from Cyprinus carpio[J].Chinese journal of preventive veterinary medicine,2010,(12):981-983.[doi:10.3969/j.issn.1008-0589.2010.12.18]
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框镜鲤致病性维氏气单胞菌的分离鉴定
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2010年12期
页码:
981-983
栏目:
研究简报
出版日期:
2010-12-01

文章信息/Info

Title:
Isolation and identification of pathogenic Aeromonas veronii from Cyprinus carpio
文章编号:
1008-0589(2010)12-0981-03
作者:
龚 倩1高淑琴2单晓枫1郭伟生1孟庆峰3王伟利3*钱爱东1*
1. 吉林农业大学 动物科学技术学院,吉林 长春 130118;
2. 吉林省东辽县卫生学校,吉林 东辽 136600;3. 吉林出入境检验检疫局,吉林 长春 130062
Author(s):
GONG Qian1 GAO Shu-qin2 SHAN Xiao-feng1 GUO Wei-sheng1 MENG Qing-feng3 WANG Wei-li3* QIAN Ai-dong1*
1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;
2. Jilin Dongliao Health School, Dongliao 136600, China; 3. Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062, China
关键词:
框镜鲤维氏气单胞菌分离鉴定
Keywords:
Cyprinus carpio Aeromonas veronii isolation and identification
分类号:
S852.61
DOI:
10.3969/j.issn.1008-0589.2010.12.18
文献标志码:
A
摘要:
为鉴定一株致框镜鲤发病病原菌的生物学特性,本研究由濒死框镜鲤体内分离到一株细菌CY0806,对形态学、培养特性、理化特性等生物学特性以及药物敏感性和16S rRNA序列进行检测和分析。结果显示:细菌株CY0806为革兰氏阴性菌,其理化特性与维氏气单胞菌基本相同。系统进化树分析表明,细菌株CY0806序列与维氏气单胞菌模式株ATCC35624无差异,同源性为100 %,因此,鉴定该菌株CY0806为维氏气单胞菌。药物敏感性试验结果显示,庆大霉素、新霉素等11种药物对细菌株CY0806有较好的抑菌效果。
Abstract:
A pathogenic bacterial isolate CY0806 was isolated from naturally infected Cyprinus carpio and identified according to morphology, cultural character, physical and chemical characters, 16S rRNA sequence analysis methods and drug susceptibility. The results showed that isolate CY0806 was gram negative and the physical and chemical character were identical with A. veronii. Sequence of the bacteria has 100% similarity with that of reference Aeromonas strain ATCC35624. These results demonstrated that isolate CY0806 was A. veronii. Drug susceptibility test results showed that isolate CY0806 was sensitive to gentamicin, neomycin and other 11 drugs.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2010-05-23
基金项目:吉林省科技厅项目(20080218);吉林农业大学青年启动基金
作者简介:龚 倩(1964-),吉林长春人,女,副教授,主要从事动物医学的科研与教学工作.
*通信作者:E-mail:qianaidong0115@163.comwang-weili@163.com
更新日期/Last Update: 2010-12-13